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Quantitative real-time PCR method for rapid enumeration of Enterobacteriaceae in food

Research Project

Project/Area Number 22780191
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

TAKAHASHI Hajime  東京海洋大学, 海洋科学技術研究科助教, 助教 (40413116)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords食品衛生 / 食品微生物 / 遺伝子 / 腸内細菌科菌群 / リアルタイムPCR
Research Abstract

In food manufacturing companies, the bacteriological examination of foods targets not only foodborne pathogens but total aerobic bacterial counts and hygiene indicator bacteria as well. However, it is hard to establish an examination method using real-time PCR for total aerobic bacteria and hygiene indicator bacteria, since the method needs to target several different bacterial species at the same time. In this study, we developed a rapid detection and quantification method for Enterobacteriaceae using quantitative real-time PCR.First of all, several DNA regions were investigated in order to design PCR primers for specific detection of Enterobacteriaceae. As a result, primers designed within the rplP gene could specifically detect Enterobacteriaceae.Next, the accuracy of the real-time PCR method was confirmed by comparing with a culture method. The difference between these two methods was within one order. These results indicate that the real-timePCR developed in this study could accurately and rapidly quantify Enterobacteriaceae in foods.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2012 2011

All Presentation (4 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation] Bon Kimura Quantitative real-time PCR method for rapid enumeration of Enterobacteriaceae in food2012

    • Author(s)
      Hajime Takahashi, Yuichiro Tanaka, Rumi Sito, Takashi Kuda
    • Organizer
      International ICFMH symposiumFoodMicro2012
    • Place of Presentation
      Istanbul
    • Related Report
      2012 Final Research Report
  • [Presentation] Quantitative real-time PCR method for rapid enumeration of Enterobacteriaceae in food2012

    • Author(s)
      Hajime Takahashi
    • Organizer
      International ICFMH symposium FoodMicro2012
    • Place of Presentation
      Istanbul
    • Related Report
      2012 Annual Research Report
  • [Presentation] PCR法を用いた腸内細菌科菌群の迅速検出法の検討2011

    • Author(s)
      斉藤瑠美,田中悠一郎, 高橋 肇,久田 孝,木村 凡
    • Organizer
      第102回日本食品衛生学会学術講演会
    • Place of Presentation
      秋田
    • Year and Date
      2011-09-29
    • Related Report
      2012 Final Research Report
  • [Presentation] PCR法を用いた腸内細菌科菌群の迅速検出法の検討2011

    • Author(s)
      斉藤瑠美
    • Organizer
      第102回日本食品衛生学会学術講演会
    • Place of Presentation
      秋田ビューホテル
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 腸内細菌科菌群検出方法2012

    • Inventor(s)
      高橋 肇 他
    • Industrial Property Rights Holder
      国立大学法人 東京海洋大学
    • Industrial Property Number
      2012-035637
    • Filing Date
      2012-02-21
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 腸内細菌科菌群検出方法2012

    • Inventor(s)
      高橋肇, 他
    • Industrial Property Rights Holder
      東京海洋大学
    • Industrial Property Number
      2012-035637
    • Filing Date
      2012-02-21
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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