Quantitative real-time PCR method for rapid enumeration of Enterobacteriaceae in food
| Project/Area Number |
22780191
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Fisheries chemistry
|
|---|
| Research Institution | Tokyo University of Marine Science and Technology |
|---|
Principal Investigator |
TAKAHASHI Hajime 東京海洋大学, 海洋科学技術研究科助教, 助教 (40413116)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 食品衛生 / 食品微生物 / 遺伝子 / 腸内細菌科菌群 / リアルタイムPCR |
|---|
| Research Abstract |
In food manufacturing companies, the bacteriological examination of foods targets not only foodborne pathogens but total aerobic bacterial counts and hygiene indicator bacteria as well. However, it is hard to establish an examination method using real-time PCR for total aerobic bacteria and hygiene indicator bacteria, since the method needs to target several different bacterial species at the same time. In this study, we developed a rapid detection and quantification method for Enterobacteriaceae using quantitative real-time PCR.First of all, several DNA regions were investigated in order to design PCR primers for specific detection of Enterobacteriaceae. As a result, primers designed within the rplP gene could specifically detect Enterobacteriaceae.Next, the accuracy of the real-time PCR method was confirmed by comparing with a culture method. The difference between these two methods was within one order. These results indicate that the real-timePCR developed in this study could accurately and rapidly quantify Enterobacteriaceae in foods.
|
Report
(4 results)
Research Products
(6 results)
