| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Fluorescent calcein staining successfully visualized cleithrum, and dentary, gill arch and neurocranium of yellowtail at 2 and 14 days post hatching(dph), respectively. Calcein staining also visualized neural spine and other elements of back bone around 15 dph. However, pigment formation at the surface of fish body hinder direct observation of back bone of fish at around 18 dph. Moreover, thickening of body often prevented direct observation of axial skeleton in fish at around 22 dph and becomes impossible at 27 dph. To analyze coincidence of growth depression and occurrence of skeletal deformity, occurrence of bone deformity was examined in fish showing different sizes from same batch of fish. in the 1^<st> experiment, significantly higher incidence of skeletal defomrity was observed in fish from bigger size of fish. However, in apparent contrat, smaller size fish showed higher frequency of occurrence of skeletal deformity. Therefore, there was no clear relationship between growth rate and occurrence of skeletal deformity in fish.
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