Molecular mechanism of environmental stress-induced apoptosis in fish
| Project/Area Number |
22780193
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Nihon University |
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Principal Investigator |
YABU Takeshi 日本大学, 生物資源科学部, ポストドクトラルフェロー (00551756)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 脂質生化学 / 酵素 / ストレス / アポトーシス / 水産学 / 生化学 / スフィンゴ脂質 / セラミド / リン酸化 / 脂質 / シグナル伝達 / スフィンゴミエリナーゼ |
|---|
| Research Abstract |
The neutral sphingomyelinase １ (nSMase1) is involved in environmental stress-i duced apoptosis infish. To reveal the molecular mechanism, we examined the invo vement of the stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) system. Upon heat shock treatment, the protein kinase initiated a signal transduction pathway leading to cell death (apoptosis). Using recombinant JNK1 protein, we found that phosphorylation of nSMase1 occurred at serine-270. Treatment with heat shock rapidly enhanced the nSMase1-directed kinase activity of endogenous JNK. On the other hand, the mutant of JNK1 dominant negative was much less sensitve to heat shock. Our results strongly suggest that phosphorylation of nSMase1 in heat-shock-induced apoptosis is executed by JNK.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Selenoneine, a Novel Selenium-Containing Compound, Mediates Detoxification Mechanisms against Methylmercury Accumulation and Toxicity in Zebrafish Embryo.2013
Author(s)
M. Yamashita, Y. Yamashita, T. Suzuki, Y. Kani, N. Mizusawa, S. Imamura, K. Takemoto, T. Hara, Md. A. Hossain, T. Yabu, K. Touhata.
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Journal Title
Mar. Biotechnol.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Antiviral protection mechanism mediated by Ginbuna crucian carp interferon gamma isoforms 1 and 2 throtwo distinct interferon-gamma-receptors.2011
Author(s)
T. Yabu, H. Toda, Y. Shibasaki, M. Yamashita, H. Anzai, N. Mano, Y. Masuhiro, S. Hanazawa, H. Shiba, T. Moritomo, T. Nakanishi.
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Journal Title
J.Biochem.
Volume: 150(6)
Pages: 635-648
Related Report
Peer Reviewed
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[Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish.2011
Author(s)
H. Toda, Y. Saito, T. Koike, F. Takizawa,K. Araki, T. Yabu, T. Somamoto, H. Suetake, Y. Suzuki, M. Ototake, T. Moritomo, T. Nakanishi.
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Journal Title
Dev .Comp.Immunol.
Volume: 35(6)
Pages: 650-660
Related Report
Peer Reviewed
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[Journal Article] Antiviral protection mechanism mediated by Ginbuna crucian carp interferon gamma isoforms 1 and 2 through two distinct interferon-gamma-receptors2011
Author(s)
T.Yabu, H.Toda, Y.Shibasaki, M.Yamashita, H.Anzai, N.Mano, Y.Masuhiro, S.Hanazawa, H.Shiba, T.Moritomo, T.Nakanishi
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Journal Title
J.Biochem.
Volume: 150
Pages: 635-648
NAID
Related Report
Peer Reviewed
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[Journal Article] Selenoneine,a NovelSelenium-Containing compound, Mediates Detoxification Mechanisms against Methylmercury Accumulation and Toxicity in Zebrafish Embryo.
Author(s)
M. Yamashita, Y. Yamashita, T. Suzuki, Y. Kani, N. usawa, S. Imamura, K. Takemoto, T. Hara, Md. A. Hossain, T Yabu K. Touhata.
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Journal Title
Mar.Biotechnol.
Volume: (in press)
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] リン酸化中性スフィンゴミエリナーゼ1,抗リン酸化中性スフィンゴミエリナーゼ1抗体,中性スフィンゴミエリナーゼ1変異体,及びそれらの用途。2012
Inventor(s)
山下倫明,今村伸太朗,藪健史,東畑顕,石原賢司
Industrial Property Rights Holder
山下倫明,今村伸太朗,藪健史,東畑顕,石原賢司
Industrial Property Number
2012-272595
Filing Date
2012-12-13
Related Report
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