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Development of functional foods and feeds using probiotic lactic acid bacteria DNA

Research Project

Project/Area Number 22780239
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Zootechnical science/Grassland science
Research InstitutionShinshu University

Principal Investigator

SHIMOSATO Takeshi  信州大学, ファイバーナノテク国際若手研究者育成拠点, 助教 (00467200)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords乳酸菌 / オリゴヌクレオチド / 機能性食品 / 機能性飼料 / プロバイオティクス / DNA / 食品免疫学
Research Abstract

We found the strong immunostimulatory effects of an oligodeoxynucleotide from lactic acid bacteria, designated MsST. We show that 24-hour of stimulation with MsST in mouse immune cells strongly induces expression of interleukin(IL)-33, a cytokine in the IL-1 superfamily. We synthesized biodegradable capsule containing oligodeoxynucleotide, which showed sustaining-hydrolyzed and preferably degraded in acidic condition. Thereby, we could extend our studies in the oral administrative inhaled approach in intestinal inflammation study.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (39 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (29 results) Book (2 results)

  • [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway2012

    • Author(s)
      Shimazu, T., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
    • Journal Title

      Infect.Immun.

      Volume: 80 Issue: 1 Pages: 276-288

    • DOI

      10.1128/iai.05729-11

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues2011

    • Author(s)
      Tohno, M., H.Aso, H.Kitazawa, et al
    • Journal Title

      Vet.Immunol.Immunopathol.

      Volume: 144 Issue: 3-4 Pages: 410-416

    • DOI

      10.1016/j.vetimm.2011.09.010

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells2011

    • Author(s)
      Hosoya, S., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
    • Journal Title

      Vet.Res.

      Volume: 42(111) Issue: 1 Pages: 1-13

    • DOI

      10.1186/1297-9716-42-111

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Toll-like receptor-2 activating bifidobacteria strains differentially regulate inflammatory cytokines in porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics2011

    • Author(s)
      Fujie, H., H.Aso, Y.Suda, T.Saito, H.Kitazawa, et al
    • Journal Title

      FEMS Immunol.Med.Microbiol.

      Volume: 63 Issue: 1 Pages: 129-139

    • DOI

      10.1111/j.1574-695x.2011.00837.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Lactobacillus pentosus ONRIC b0240 on intestinal IgA production in mice fed differing levels of protein2011

    • Author(s)
      Shimosato T, Tomida K, Otani H
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 59(6) Issue: 6 Pages: 2646-2651

    • DOI

      10.1021/jf104240d

    • Related Report
      2011 Final Research Report
  • [Journal Article] Anti-allergic effects of a mixture of Saccharomyces cerevisiae and its specific goat' s milk immunoglobulin G-rich fraction on ovalbuminsensitized BALB/c mice2011

    • Author(s)
      Sekimura Y, Shimosato T, Otani H
    • Journal Title

      Milchwissenshaft-Milk Science International

      Volume: 66(1) Pages: 7-10

    • Related Report
      2011 Final Research Report
  • [Journal Article] Accelerated Wound Healing Mediated by Activation of Toll-like Receptor 92010

    • Author(s)
      Sato T, Yamamoto M, Shimosato T, KlinmanDM
    • Journal Title

      Wound Repair and Regeneration

      Volume: 18(6) Issue: 6 Pages: 586-593

    • DOI

      10.1111/j.1524-475x.2010.00632.x

    • NAID

      10031181701

    • Related Report
      2011 Final Research Report
  • [Journal Article] イムノジェニクスとしての免疫刺激性DNA研究の最前線2010

    • Author(s)
      下里剛士, 北澤春樹
    • Journal Title

      北信越畜産学会報

      Volume: 100 Pages: 1-9

    • NAID

      40017293435

    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸菌由来オリゴヌクレオチドによる新規分泌型IL-33様タンパク質の誘導2012

    • Author(s)
      下里剛士, 伊藤雄亮, 遠野雅徳, 大谷元, 北澤春樹
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      愛知県名古屋市名古屋大学東山キャンパス
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 乳酸菌由来オリゴヌクレオチドによる新規分泌型IL-33様タンパク質の誘導2012

    • Author(s)
      下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] ビール酵母とその牛乳抗体のマウスI型アレルギーに及ぼす影響2012

    • Author(s)
      森崎健太郎,松田孝裕,岡田実,堀江健二,金武祚,下里剛士,大谷元
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ後期初乳の新規生体防御機能の探索2012

    • Author(s)
      長谷川実希,遠見和幸,内田健志,為定誠,下里剛士,大谷元
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ後期初乳中の抗アレルギータンパク質の探索2012

    • Author(s)
      遠見和幸,須賀正喜,内田健志,為定誠,下里剛士,大谷元
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸菌菌体外多糖の異なるパターン認識受容体を介する免疫調節機能特性2012

    • Author(s)
      室伏陽,和知俊,細矢翔一,遠野雅徳,島津朋之,下里剛士,川井泰,齋藤忠夫,橋口健司,吉田睦子,北澤春樹
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ後期初乳添加飼料で飼育したマウスのアレルギー関連因子に及ぼす影響2011

    • Author(s)
      遠見和幸,清水努,内田健志,為定誠,下里剛士,大谷元
    • Organizer
      第60回北信越畜産学会大会
    • Place of Presentation
      長野
    • Year and Date
      2011-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] ビール酵母とそれに特異的な牛乳IgGのマウスI型アレルギーに及ぼす影響2011

    • Author(s)
      森崎健太郎,下里剛士,大谷元
    • Organizer
      第60回北信越畜産学会大会
    • Place of Presentation
      長野
    • Year and Date
      2011-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Streptococcus thermophilus由来オリゴヌクレオチドにより誘導されるIL-33活性化機構の解析2011

    • Author(s)
      下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
    • Organizer
      平成23年度日本酪農科学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] αS1カゼインを発現するLactococcus lactis組み換え体の構築2011

    • Author(s)
      下里剛士,重盛駿,大谷元
    • Organizer
      平成23年度日本酪農科学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体防御に関わるペプチドグリカン認識タンパク質ファミリーの腸管における発現解析2011

    • Author(s)
      遠野雅徳,植田渉,島津朋之,下里剛士,麻生久,北澤春樹
    • Organizer
      平成23年度日本酪農科学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ腸管上皮細胞による抗ウィルス性イムノバイオティクスの評価系構築2011

    • Author(s)
      千葉絵里子,細矢翔一,高梨直也,下里剛士,遠野雅徳,麻生久,川井泰,齋藤忠夫,北澤春樹
    • Organizer
      平成23年度日本酪農科学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Streptococcus thermophilus由来オリゴヌクレオチドにより誘導されるIL-33活性化機構の解析2011

    • Author(s)
      下里剛士, 伊藤雄亮, 遠野雅徳, 大谷元, 北澤春樹
    • Organizer
      平成23年度日本酪農科学シンポジウム
    • Place of Presentation
      宮城県仙台市フォレスト仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Streptococcus thermophilus由来オリゴヌクレオチド刺激によるIL-33の断片化解析2011

    • Author(s)
      下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ後期初乳および常乳のマウスI型アレルギーに及ぼす影響2011

    • Author(s)
      遠見和幸,清水努,内田健志,為定誠,下里剛士,大谷元
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 腸管免疫系におけるイムノバイオティック乳酸菌によるNLRP3受容体の発現増強2011

    • Author(s)
      遠野雅徳,下里剛士,麻生久,北澤春樹
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ腸管上皮細胞による抗ウイルス性イムノバイオティクスの評価系構築2011

    • Author(s)
      千葉絵里子,細矢翔一,高梨直也,下里剛士,遠野雅徳,麻生久,川井泰,齋藤忠夫,北澤春樹
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Streptococcus thermophilus由来オリゴヌクレオチド刺激によるIL-33の断片化解析2011

    • Author(s)
      下里剛士, 伊藤雄亮, 遠野雅徳, 大谷元, 北澤春樹
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      青森県十和田市北里大学十和田キャンパス
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Toll-like receptor 4 recognizes extracellular polysaccharide from Lactococcus lactis subsp. cremoris2011

    • Author(s)
      Tohno M, Shimosato T, Aso H, Kitazawa H
    • Organizer
      4th congress of european microbiologists FEMS 2011
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2011 Final Research Report
  • [Presentation] CpG oligodeoxynucleotides promote wound healing in airway epithelial cells2011

    • Author(s)
      Sato T, Miyazawa N, Klinman DM, Shimosato T, Takahashi R, Tsukahara T, Hayashi M, Yamamoto M, Tomaru K, Kaneko T, Ishigatsubo Y
    • Organizer
      American Thoracic Society Annual Meeting 2011
    • Place of Presentation
      Denver, CO, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Suppressive Oligodeoxynucleotide Synergistically Enhances The Antiproliferative Effect Of Anti-cancer Drug In Human Lung Cancer Cells2011

    • Author(s)
      Takahashi R, Sato S, Miyazawa N, Klinman DM, Shimosato T, Hayashi M, Yamamoto M, Tomaru K, Tsukahara T, Kaneko T, Ishigatsubo Y
    • Organizer
      American Thoracic Society Annual Meeting 2011
    • Place of Presentation
      Denver, CO, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] プルーン果実の免疫調節機能の探索2011

    • Author(s)
      宮下亮二,唐澤幸司,下里剛士,大谷元
    • Organizer
      2011年度日本栄養食糧学会大会講演要旨
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Streptococcusthermophilus由来CpG DNAが誘導するIL-33の機能性解析2010

    • Author(s)
      下里剛士,畑野木の実,藤本恵,遠野雅徳,大谷元,北澤春樹
    • Organizer
      平成22年度日本酪農科学シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-08-20
    • Related Report
      2011 Final Research Report
  • [Presentation] The novel immunostimulatory activities of CpG oligodeoxynucleotides from Streptococcus thermophilus2010

    • Author(s)
      Shimosato T, Fujimoto M, Tohno M, SatoT, Otani H, Kitazawa H
    • Organizer
      ADSA-PSA-AMPA-CSAS-ASAS Joint annual meeting
    • Place of Presentation
      CO, USA.
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of immunobiotics on the immune performance and productivity of pigs2010

    • Author(s)
      Suda Y, Takahashi Y, Tohno M, Villena Julio, Hosoya S, Fujie H, Shimosato T, Kobashi J, Ishida M, Aso H, Kawai Y, Saito T, Makino S, Ikegami S, Kitazawa H
    • Organizer
      International Scientific Conference on Probiotics and Prebiotics-IPC2010
    • Place of Presentation
      Kosice, Slovakia
    • Related Report
      2011 Final Research Report
  • [Presentation] Evaluation of Anti-Inflammatory Immunobiotics in Porcine Intesinal Epithelial Cells2010

    • Author(s)
      Kitazawa H, Shimazu T, Tohno M, Villena J, Hosoya S, Fujie H, Suda Y, Shimosato T, Aso H, Makino S, Ikegami S, Kawai Y, Saito T
    • Organizer
      International Scientific Conference on Probiotics and Prebiotics-IPC2010
    • Place of Presentation
      Kosice, Slovakia
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸菌由来CpG DNAによるToll様受容体9を介するIL-33の発現増強2010

    • Author(s)
      下里剛士,遠野雅徳,大谷元,北澤春樹
    • Organizer
      日本食品免疫学会第6回学術集会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Accelerated wound healing in mice by local administration of sustained-release CpG oligodeoxynucleotide2010

    • Author(s)
      Sato T, Shimosato T, Klinman DM
    • Organizer
      97th AAI Annual Meeting, IMMUNOLOGY 2010
    • Place of Presentation
      Baltimore, MD, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸菌由来CpG DNAによるToll様受容体9を介するIL-33の発現増強2010

    • Author(s)
      下里剛士
    • Organizer
      日本食品免疫学会第6回学術大会
    • Place of Presentation
      東京大学安田講堂
    • Related Report
      2010 Annual Research Report
  • [Book] アジュバント開発研究の新展開第三章アジュバント各論「プロバイオティック乳酸菌を利用した経口ワクチン・アジュバント研究の新展開」2011

    • Author(s)
      石井健,青枝大貴,小檜山康司,鉄谷耕平,審良静男,山西弘一,吉田裕樹,原博満,安田好文,中西憲司,竹内理,荒木幸一,黒田悦史,小山正平,藤本ゆかり,深瀬浩一,新川武,宮田健,柳義和,豊永憲司,山崎晶,下里剛士,北澤春樹, CevayirCoban, KeiichiOhata, YoshikatsuIgai, MasahiroKato, ToshihiroTsukui,望月慎一,櫻井和朗,岩田晃,前山順一,山本三郎,改正恒康,西川元也,高倉喜信,角田慎一,堤康央,吉開泰信,内田哲也,赤木隆美,明石満,長谷川秀樹,植松智,佐々木津, NathalieGarcon,瀬谷司,佐藤治子,志馬寛明,松本美佐子
    • Publisher
      株式会社シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] アジュバント開発研究の新展開Advanced Technologies for Adjuvant Research and Development2011

    • Author(s)
      石井健, 下里剛士, 他43名
    • Total Pages
      249
    • Publisher
      株式会社シーエムシー出版
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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