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Proposal of new screening method of adhesive probiotic lactic acid bacteria by enzymic activity

Research Project

Project/Area Number 22780241
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Zootechnical science/Grassland science
Research InstitutionMiyagi University

Principal Investigator

KINOSHITA Hideki  宮城大学, 食産業学部, 助教 (50533288)

Co-Investigator(Renkei-kenkyūsha) SAITO Tadao   (00118358)
ISHIDA Mitsuharu   (40151386)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords乳酸菌 / 腸管付着性 / 付着因子 / アドヘシン / 酵素活性 / プロバイオティクス / ムチン / 熱ショックタンパク質 / GroEL / 酵素 / スクリーニング / グリセルアルデヒド-3-リン酸脱水素酵素
Research Abstract

Our aim was to construct a new screening method for highly adhesive lactobacilli based on an enzymatic activity without using the adhesion test. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface GAPDH activity. GAPDH activities were detected in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. Thenew screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the positive correlation of GAPDH activity with adhesion of lactobacilli.Moreover, the heat shock protein, GroEL, was also detected in many lactobacilli. This suggests GAPDH and GroEL are a very important protein not only in the cytoplasm but also on the cell surface and may be a universal adhesin for lactobacilli.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (27 results)

All 2013 2012 2011 2010 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (14 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)2013

    • Author(s)
      H. Kinoshita, S. Imoto, Y. Suda, M. Ishida, M. Watanabe, Y. Kawai, H. Kitazawa, K. Miura, A. Horii, T. Saito
    • Journal Title

      Animal Science Journal

      Volume: 84巻 Pages: 150-158

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)2013

    • Author(s)
      Kinoshita H, Imoto S, Suda Y, Ishida M, Watanabe M, Kawai Y, Kitazawa H, Miura K, Horii A, Saito T.
    • Journal Title

      Anim Sci J.

      Volume: 84 Issue: 2 Pages: 150-158

    • DOI

      10.1111/j.1740-0929.2012.01054.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An Adhesin-Like Protein, Lam29, from Lactobacillus mucosae ME-340 Binds to Histone H3 and Blood Group Antigensin Human Colonic Mucus、Bioscience2012

    • Author(s)
      M. Watanabe, H.Kinoshita, IN. Huang, K. Eguchi, T. Tsurumi, Y. Kawai, H. Kitazawa, K. Kimura, N. Taketomo, D. Kikuchi, T. Sase, K. Miura, H. Ogawa, C. Shibata, A. Horii, T. Saito
    • Journal Title

      Biotechnology, and Biochemistry

      Volume: 76巻 Pages: 1655-1660

    • URL

      http://dx.doi.org/10.1271/bbb.120150

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 有用乳酸菌の単離と腸管付着メカニズムの解明に関する研究2012

    • Author(s)
      木下英樹
    • Journal Title

      ミルクサイエンス

      Volume: 61巻 Pages: 217-228

    • NAID

      130003398722

    • URL

      http://ci.nii.ac.jp/naid/40019556814

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] An Adhesin-Like Protein, Lam29, from <i>Lactobacillus mucosae</i> ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus2012

    • Author(s)
      Watanabe M, Kinoshita H, Huang IN, Eguchi K, Tsurumi T, Kawai Y, Kitazawa H, Kimura K, Taketomo N, Kikuchi D, Sase T, Miura K, Ogawa H, Shibata C, Horii A, Saito T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 9 Pages: 1655-1660

    • DOI

      10.1271/bbb.120150

    • NAID

      130004137955

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MRS寒天培地と変法LBS寒天培地における乳酸菌の選択的単離能の比較2011

    • Author(s)
      木下英樹,井本瞬,須田義人,石田光晴
    • Volume
      60巻
    • Pages
      171-176
    • NAID

      130003398707

    • URL

      http://ci.nii.ac.jp/naid/40019173279

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] MRS寒天培地と変法LBS寒天培地における乳酸菌の選択的単離能の比較2011

    • Author(s)
      木下英樹, 井本瞬, 須田義人, 石田光晴
    • Journal Title

      ミルクサイエンス誌

      Volume: 60 Pages: 171-176

    • NAID

      130003398707

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens2010

    • Author(s)
      M. Watanabe, H. Kinoshita, M. Nitta, R. Yukishita, Y. Kawai, K. Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, K. Miura, A. Horii, H. Kitazawa and T. Saito
    • Journal Title

      Journal of applied Microbiology

      Volume: 109巻 Issue: 3 Pages: 927-935

    • DOI

      10.1111/j.1365-2672.2010.04719.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] プロバイオティック乳酸菌の酵素活性を有する腸管付着因子とその腸管付着メカニズム2010

    • Author(s)
      木下英樹
    • Journal Title

      宮城大学食産業学部紀要

      Volume: 4巻 Pages: 45-50

    • NAID

      120006015011

    • URL

      http://ci.nii.ac.jp/naid/40017144924

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens2010

    • Author(s)
      M.Watanabe, H.Kinoshita, et al
    • Journal Title

      Journal of Applied Microbiology

      Volume: 109 Pages: 927-935

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Lactobacillus mucosae由来アドへシン様タンパク質(Lam29)の新規ヒト大腸粘液由来結合因子の探索および同定2012

    • Author(s)
      渡辺真通, 木下英樹,川井泰,北澤春樹,三浦康,柴田近,堀井明,木村勝紀,竹友直生,齋藤忠夫
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋(名古屋大学東山キャンパス)
    • Year and Date
      2012-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Lactobacillus mucosae由来アドヘシン様タンパク質(Lam29)の新規ヒト大腸粘液由来結合因子の探索および同定2012

    • Author(s)
      渡辺真通、木下英樹, ら
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Discovery of a new adhesin-like protein from Lactobacillus mucosae ME-340 with binding activity to human blood group A and B antigens2010

    • Author(s)
      M. Watanabe, H. Kinoshita, M. Nitta, R. Yukishita, M. Haruta, K. Eguchi, Y. Kawai, K. Kimura, N. Taketomo, K. Miura, A. Horii, H. Kitazawa, T. Saito
    • Organizer
      The 14th AAAP Animal Science Congress (AAAP2010)
    • Place of Presentation
      National Pingtung University of Science and Technology、台湾
    • Year and Date
      2010-08-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Search for new Bifidobacterium adhesins with specific binding activity to human colonic mucin2010

    • Author(s)
      R. Yukishita, M. Watanabe, M. Nitta, H. Kinoshita, Y. Kawai, K. Miura, A. Horii, J. Z. Xiao, T. Yaeshima, K. Iwatsuki, H. Kitazawa, T. Saito
    • Organizer
      The 14th AAAP Animal Science Congress (AAAP2010)
    • Place of Presentation
      National Pingtung University of Science and Technology、台湾
    • Year and Date
      2010-08-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Discovery of a new adhesin-like protein from Lactobacillus mucosae ME-340 with binding activity to human blood group A and B antigens2010

    • Author(s)
      Watanabe, Kinoshita, et al
    • Organizer
      The 14th AAAP Animal Science Congress
    • Place of Presentation
      台湾(屏東科学技術大学)
    • Year and Date
      2010-08-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Search for new Bifidobacterium adhesins with specific binding activity to human colonic mucin2010

    • Author(s)
      Yukishita, Kinoshita, et al
    • Organizer
      The 14th AAAP Animal Science Congress
    • Place of Presentation
      台湾(屏東科学技術大学)
    • Year and Date
      2010-08-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] A new method to screen for lactobacilli adhesive to human colonic mucin2010

    • Author(s)
      H. Kinoshita, S. Imoto, Y. Suda, M. Ishida, M. Watanabe, M. Nitta, R. Yukishita, Y. Kawai, H. Kitazawa, K. Miura, A. Horii, T. Saito
    • Organizer
      The 14th AAAP Animal Science Congress (AAAP2010)
    • Place of Presentation
      National Pingtung University of Science and Technology、台湾
    • Year and Date
      2010-08-24
    • Related Report
      2012 Final Research Report
  • [Presentation] A new method to screen for lactobacilli adhesive to human colonic mucin2010

    • Author(s)
      Kinoshita, et al
    • Organizer
      The 14th AAAP Animal Science Congress
    • Place of Presentation
      台湾(屏東科学技術大学)
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] GAPDH 酵素活性を指標とした腸管付着性乳酸菌の新規スクリーニング法の検討2010

    • Author(s)
      木下英樹 ,井本瞬, 須田義人,石田光晴,三浦康,堀井明,渡辺真通,新田将光,雪下玲奈, 川井泰,北澤春樹,齋藤忠夫
    • Organizer
      日本乳酸菌学会 2010 年度大会
    • Place of Presentation
      仙台(フォレスト仙台)
    • Year and Date
      2010-07-27
    • Related Report
      2012 Final Research Report
  • [Presentation] GAPDH酵素活性を指標とした腸管付着性乳酸菌の新規スクリーニング法の検討2010

    • Author(s)
      木下, ら
    • Organizer
      日本乳酸菌学会2010年度大会
    • Place of Presentation
      フォレスト仙台
    • Year and Date
      2010-07-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト ABO 式血液型抗原を介したプロバイオティック乳酸菌の腸管付着性機構に関する研究2010

    • Author(s)
      渡辺真通,新田将光,雪下玲奈,春田美由樹,江口慧,川井泰,北澤春樹,齋藤忠夫,木下英樹,三浦康,堀井明,木村勝紀,竹友直生
    • Organizer
      日本乳酸菌学会 2010 年度大会
    • Place of Presentation
      仙台(フォレスト仙台)
    • Year and Date
      2010-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ビフィズス菌の新規ヒト腸管付着性因子(アドヘシン)の探索と付着機構の解明2010

    • Author(s)
      雪下玲奈,渡辺真通,新田将光,木下英樹,川井泰,北澤春樹,三浦康,堀井明,清水(肖)金忠,八重島智子,岩附慧二,齋藤忠夫
    • Organizer
      日本乳酸菌学会 2010 年度大会
    • Place of Presentation
      仙台(フォレスト仙台)
    • Year and Date
      2010-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトABO式血液型抗原を介したプロバイオティック乳酸菌の腸管付着性機構に関する研究2010

    • Author(s)
      渡辺、木下, ら
    • Organizer
      日本乳酸菌学会2010年度大会
    • Place of Presentation
      フォレスト仙台
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビフィズス菌の新規ヒト腸管付着性因子(アドヘシン)の探索と付着機構の解明2010

    • Author(s)
      雪下、木下, ら
    • Organizer
      日本乳酸菌学会2010年度大会
    • Place of Presentation
      フォレスト仙台
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Book] 乳酸菌とビフィズス菌のサイエンス2010

    • Author(s)
      木下英樹、齋藤忠夫
    • Publisher
      京都大学学術出版会
    • Related Report
      2012 Final Research Report
  • [Book] 乳酸菌とビフィズス菌のサイエンス2010

    • Author(s)
      木下, ら
    • Total Pages
      668
    • Publisher
      京都大学学術出版会
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.myu-syoku.jp/university/faculty/Introduction/index/introductionID/51

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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