| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
High blood pressure injures blood vessels and can cause cerebral and myocardial infarction. In our previous studies, chicken collagen hydrolysate has shown antihypertensive effects in spontaneously hypertensive rats(SHRs) and mildly hypertensive human subjects. Moreover, our recent study showed that chicken collagen hydrolysate ameliorates vascular injury through endothelial progenitor cell(EPC) activation. The purpose of our current study was twofold : first, to observe whether we could obtain the same results as above using different live resources and/or native gelatin ; and second, to clarify the mechanism of action of chicken collagen hydrolysate. SHRs were fed chicken collagen hydrolysate, porcine collagen hydrolysate, or chicken gelatin for 7 weeks. EPCs, sera, organs(liver, heart, kidneys), and vascular tissues were collected from the SHRs. Kidney markers were investigated to clarify the effect of administration of each substance on the kidney. mRNAs were isolated from the kidney and heart to measure the expression levels of various cytokines. On administration, none of the substances caused changes in the levels of markers of renal injury except for porcine collagen hydrolysate(TIMP-1). However, chicken collagen hydrolysate increased the mRNA levels of the EPC markers CD34 and Flk-1.Casein hydrolysate significantly elevated the mRNA levels of Flk-1 and NOS3.
To clarify the mechanisms by which these two substances induced EPC activation, EPCs were cultured with sera prepared from rats fed the test substances. Chicken collagen hydrolysate enhanced EPC colony formation, and casein hydrolysate promoted early vasogenesis.
These results suggest that administration of chicken collagen hydrolysate activates EPC migration, and that administration of casein hydrolysate induces the differentiation of EPCs into endothelial cells.
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