Studies on the transcriptional regulation of fructan exohydrolase genes during regrowth after cutting in forage grasses

Research Project

Project/Area Number	22780244
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Zootechnical science/Grassland science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	TAMURA Ken-ichi 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・酪農研究領域, 主任研究員 (10414749)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	フルクタン / フルクタン分解酵素 / 再生 / チモシー / 遺伝子発現制御 / イネ科牧草 / 遺伝子発現 / プロモータ / 酵素
Research Abstract	A fructan exohydrolase gene, Pp6-FEH1 was transcriptionally induced in basal parts of timothy after cutting. This suggests that Pp6-FEH1 is involved in the energy supply for regrowth after cutting. Glucose and sucrose inhibited the up-regulation of Pp6-FEH1 transcripts. Transcriptional induction of Pp6-FEH1 by cold also suggests its involvement in increase of freezing tolerance by producing mono-saccharides known as cryoprotectants. Reporter assay revealed the promoter region involved in the cold induction of timothy FEH.