Functional analysis of the de novo DNA methyltranserase specific for in vitro-manipulated embryos
| Project/Area Number |
22780248
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Gunma University |
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Principal Investigator |
HORII Takuro 群馬大学, 生体調節研究所, 助教 (00361387)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピジェネティクス / メチル化 / 受精卵 / 体外操作 / 体外培養 / 体外受精 |
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| Research Abstract |
In vitro-manipulated embryos, such as in vitro fertilization (IVF), in vitro culture (IVC) embryos often exhibit abnormal DNA methylation patterns in imprinted genes and centromeric and pericentromeric satellite repeats. In this study, we found an alternatively spliced variant of Dnmt3b lacking exon 5 (Dnmt3b・5) that is specific to mouse IVC embryos. In vitro methylation activity assays showed that Dnmt3b・5 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3b・5 could induce a hypo methylation status especially in vitro manipulated embryos.
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Report
(3 results)
Research Products
(19 results)
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[Presentation] 単為発生胚由来ES細胞を用いた再生医療2010
Author(s)
堀居拓郎, 木村美香, 森田純代, 日高京子, 長尾恭光, 落谷孝広, 畑田出穂
Organizer
第33回日本分子生物学会年会
Place of Presentation
兵庫県・神戸市・ポートピアホテル他
Year and Date
2010-12-09
Related Report
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