| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
In vitro-manipulated embryos, such as in vitro fertilization (IVF), in vitro culture (IVC) embryos often exhibit abnormal DNA methylation patterns in imprinted genes and centromeric and pericentromeric satellite repeats. In this study, we found an alternatively spliced variant of Dnmt3b lacking exon 5 (Dnmt3b・5) that is specific to mouse IVC embryos. In vitro methylation activity assays showed that Dnmt3b・5 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3b・5 could induce a hypo methylation status especially in vitro manipulated embryos.
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