| Project/Area Number |
22780251
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Prefectural University of Hiroshima |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 繁殖 / 卵子成熟 / 排卵 / 卵丘細胞 / 体外成熟培養 / 卵子 / 顆粒層細胞 / 産業動物 / 顆粒膜細胞 / ブタ / 体外培養 |
|---|
| Research Abstract |
During ovulation phase, granulosa cells-derived secretion factors stimulate cumulus cells, which result in completion of oocyte maturation. Since current culture system of oocyte employs only cultivation of cumulus-oocyte complexes(COCs), rate of oocyte maturation is low compared with that of oocyte collected from in vivo due to lack or insufficient of granulosa cells-derived oocyte maturation factors. In this research, we showed that TACE/ADAM17 expressed in granulosa cells and cumulus cells cleaved EGF-like factors from granulosa cells and cumulus cells, which resulted in induction of oocyte maturation via EGFR-ERK1/2 pathway in cumulus cells during ovulation process. We also investigated that PGE2-PTGER2, 4 pathways enhanced expressions of TACE/ADAM17 and EGF-like factors, thereby sustained EGFR-ERK1/2 pathway in cumulus cells during oocyte maturation. Furthermore, c-Src phosphorylated by FSH-PKC stimuli induced association with TACE/ADAM17, which required for protease activity of TACE/ADAM17 in granulosa cells and cumulus cells. Finally, optimization of expression and activation pattern of TACE/ADAM17 in cumulus cells of COCs by PGE2 or FSH-c-Src improved maturation rate of oocyte during in vitro maturation of porcine COCs
|
