Effect of microRNA-33b on adipogenesis regulated with SREBF1 in porcine adipocytes
Project/Area Number |
22780256
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied animal science
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Research Institution | National Institute of Agrobiological Sciences |
Principal Investigator |
TANIGUCHI Masaaki 独立行政法人農業生物資源研究所, 家畜ゲノム研究ユニット, 任期付研究員 (60531431)
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Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 畜産 / 遺伝子 / 脂質 / マイクロRNA / ブタ / 脂肪合成 / 遺伝子発現 / 脂肪細胞株 / SREBP-1 / PPAR |
Research Abstract |
Sterol regulatory element binding proteins(SREBP) are transcription regulator for cholesterol and fatty acid homeostasis. MicroRNA-33b(miR-33b) is conserved in an intron of SREBP-1 genes of most mammalians. A computational prediction suggested that target for miR-33b includes SREBP-cleavage activating protein(SCAP). Therefore we sought to investigate the effect of miR-33b on lipogenic pathway regulated with SCAP/SREBP-1 feedback in porcine subcutaneous pre-adipocytes(PSPA) cell line. Transfection of miR-33b decreased SCAP expression at both mRNA and protein levels by 4 d post transfection. Additionally, expression of lipogenic genes and triglyceride contents were degraded in miR-33b transfected PSPA. On the other hands, miR-33b transfection obviously decreased gene expression of peroxisome proliferator-activated receptor gamma-2(PPARγ2). Our results suggested that miR-33b is associated in part with attenuation of SCAP/SREBP-1 pathway, but also affected PPARγpathway which might consequently cause downregulation of lipogenesis in porcine adipocytes.
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Report
(3 results)
Research Products
(3 results)