High resolution transcriptome analysis in protozoan parasites

• Project/Area Number: 22780258
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tohoku University (2012); Obihiro University of Agriculture and Veterinary Medicine (2010-2011)
• Principal Investigator: YAMAGISHI Junya 東北大学, 東北メディカル・メガバンク機構, 助教 (80535328)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 原虫 / トキソプラズマ / トランスクリプトーム / 次世代シーケンサー / 転写開始点 / バイオインフォマティクス / TSS-seq / 転写制御

Research Abstract

By using RNA-seq and TSS-seq which are one of the application of next generation sequencers, it becomes possible to obtain transcriptomes of wide variety of living organisms. We applied the methods on representative three stages which consist of lifecycle of an obligate, intracellular, parasitic protozoan, Toxoplasma gondii. As a result, we demonstrated gene expression profiles of the three stages as well as putative DNA cis-elements which regulate the gene expression.

Research Products

• [Journal Article] Characterization of Toxoplasma gondii 5' UTR with encyclopedic TSS information (2012)
  • Author(s): Yamagishi, et al
  • Journal Title: J.Parasitol. Volume: 98 Issue: 2 Pages: 455-457
  • DOI: 10.1645/ge-2864.1
  • Peer Reviewed
• [Journal Article] Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice. (2012)
  • Author(s): Yu L
  • Journal Title: Parasitol Int Volume: Sep;61(3) Issue: 3 Pages: 481-486
  • DOI: 10.1016/j.parint.2012.04.001
  • Peer Reviewed
• [Journal Article] Construction and analysis of full-length cDNA library of Cryptosporidium parvum (2011)
  • Author(s): Yamagishi J, Wakaguri H, Sugano S, Kawano S, Fujisaki K, Sugimoto C, Watanabe J, Suzuki Y, Kimata I, Xuan X
  • Journal Title: Parasitol Int Volume: 60(2) Issue: 2 Pages: 199-202
  • DOI: 10.1016/j.parint.2011.03.001
  • Peer Reviewed
• [Journal Article] Full-parasites: database of full-length cDNAs of apicomplexa parasites, 2010 update (2011)
  • Author(s): Tuda J, Mongan AE, Tolba ME,Imada M, Yamagishi J, Xuan X, Wakaguri H, Sugano S, Sugimoto C, Suzuki Y. Nucleic
  • Journal Title: Acids Res Volume: 39(Database issue) Issue: Database Pages: D625-D631
  • DOI: 10.1093/nar/gkq1111
  • Peer Reviewed
• [Journal Article] Full-parasites : database of full-length cDNAs of apicomplexa parasites, 2010 update (2011)
  • Author(s): Tuda J, et al
  • Journal Title: Nucleic Acids Research Volume: 39
  • Peer Reviewed
• [Journal Article] High-resolution characterization ofToxoplasma gondii transcriptome with a massive parallel sequencing method (2010)
  • Author(s): Yamagishi J, Wakaguri H, Ueno A, Goo YK, Tolba M, Igarashi M, Nishikawa Y, Sugimoto C, Sugano S, Suzuki Y,Watanabe J, Xuan X
  • Journal Title: DNA Res Volume: 17(4) Issue: 4 Pages: 233-43
  • DOI: 10.1093/dnares/dsq013
  • Peer Reviewed
• [Journal Article] High-resolution characterization of Toxoplasma gondii transcriptome with a massive parallel sequencing method (2010)
  • Author(s): Yamagishi J, et al
  • Journal Title: DNA research Volume: 17 Pages: 233-243
  • Peer Reviewed
• [Presentation] Advances in Tropical Genomics: Conservation and Sustainable Utilization of Tropical Biodiversity (2011)
  • Author(s): Junya Yamagishi, Hiroyuki Wakaguri,Junichi Watanabe, Kawazu Shin-ichiro, Yutaka Suzuki, Xuenan Xuan
  • Organizer: Transcriptomics of a Parasit Toxoplasma gondii from Next Generation Sequencing
  • Place of Presentation: Bogor, Indonesia
• [Presentation] XIII International Congress ofBacteriology and Applied Microbiology (2011)
  • Author(s): Junya Yamagishi, Hiroyuki Wakaguri, Akio Ueno, Makoto Igarashi, Junichi Watanabe, Yutaka Suzuki, Xuenan Xuan
  • Organizer: Comparative transcriptomics among tachyzoites, bradyzoites and sporozoites of Toxoplasma gondii using massive parallel sequencing method
  • Place of Presentation: Sapporo, Japan
• [Remarks] Full-Toxoplasma
  • URL: http://fullmal.hgc.jp/index_tg_ajax.html