Study on molecular mechanisms of trypanosome cell differentiation for developing novel preventives against the parasite

• Project/Area Number: 22780269
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: SAKURAI Tatsuya 北海道大学, 大学院・獣医学研究科, 助教 (60547777)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 感染症 / 原虫 / Trypanosoma congolense / 細胞分化 / 2D-DIGE

Research Abstract

The cell differentiation from metacyclic form to bloodstream form of Trypanosoma congolense was induced in vitro, and the alternation of protein expression during the differentiation was analyzed by 2D-DIGE. The results showed that expressions of 15 proteins were increased but those of 9 proteins were decreased more than one and a half times after 6-hour induction of the differentiation. Meanwhile, expressions of 14 proteins were increased but those of 78 proteins were decreased more than twice after 12-hour induction of the differentiation.

Research Products

• [Journal Article] Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic (2012)
  • Author(s): Fujita, M., Kato, H., Gaceres, A. G., Gomez, E. A., Mimori, T., Zhang, F., Iwata, H., Korenaga, M., Sakurai, T., Katakura, K. and Hashiguchi, Y.
  • Journal Title: Acta Tropica Volume: 121 Issue: 2 Pages: 93-98
  • DOI: 10.1016/j.actatropica.2011.10.004
  • NAID: 120003860279
  • Peer Reviewed
• [Journal Article] アフリカトリパノソーマ症-獣医寄生虫学の挑戦- (2011)
  • Author(s): 櫻井達也、井上昇
  • Journal Title: 北海道獣医師会雑誌 Volume: 55 Pages: 211-217
  • URL: http://www.hokkaido-juishikai.jp/kaishi/pdf/1106-01.pdf
• [Journal Article] Structural characterization and epitope mapping of the Glutamic Acid/Alanine Rich protein from Trypanosoma congolense : Defining assembly on the parasite cell surface (2011)
  • Author(s): Loveless, B. C., Mason, J.W., Sakurai, T., Inoue, N., Razavi, M., Pearson, T. W. and Boulanger, M. J.
  • Journal Title: The Journal of Biological Chemistry Volume: 286(23) Issue: 23 Pages: 20658-20665
  • DOI: 10.1074/jbc.m111.218941
  • Peer Reviewed
• [Journal Article] Differential protein expression throughout the life cycle of Trypanosoma congolense, a major parasite of cattle in Africa (2011)
  • Author(s): Eyford, B. A., Sakurai, T., Smith, D., Loveless, B. C., Hertz-Fowler, C., Donelson, J. E., Inoue, N. and Pearson, T. W.
  • Journal Title: Molecular & Biochemical Parasitology Volume: 177 (2) Issue: 2 Pages: 116-125
  • DOI: 10.1016/j.molbiopara.2011.02.009
  • Peer Reviewed
• [Journal Article] Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids (2011)
  • Author(s): Armua-Fernandez, M. T., Nonaka, N., Sakurai, T., Nakamura, S., Gottstein, B., Deplazes, P., Phiri, I. G., Katakura, k. and Oku, Y.
  • Journal Title: Parasitology International Volume: 60(1) Issue: 1 Pages: 84-89
  • DOI: 10.1016/j.parint.2010.11.005
  • Peer Reviewed
• [Journal Article] Differential protein expression throughout lifecycle of Trypanosoma congolense, a major parasite of cattle in Africa (2011)
  • Author(s): Brett A.Eyford
  • Journal Title: Molecular and Biochemical Parasitology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids (2011)
  • Author(s): Maria Teresa Armua-Fernandez
  • Journal Title: Parasitology International Volume: 60 Pages: 84-89
  • Peer Reviewed
• [Presentation] Identification and analyses of stage-specific surface protein toward revealing of trypanosome-tsetse interaction (2010)
  • Author(s): 櫻井達也
  • Organizer: XIIth International Congress of Parasitology
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-08-17