| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
In this study, we attempted to improve hydrodynamics-based gene delivery (HGD) to achieve a highly efficient gene delivery that is targeted to the murine liver and tested whether the liver-specific expression of a gene of interest is possible. We demonstrated that upon performing HGD, mixing of plasmid DNA with polyethylenimine (PEI)-based reagent yielded best transfection efficiency in hepatocytes. With this improved technique, we succeeded in performing Cre-loxP-mediated gene switching in liver and to show lines of direct evidence for liver specific transgene expression.
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