| Project/Area Number |
22780281
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Clinical veterinary science
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
MATSUU Aya 鳥取大学, 農学部, 准教授 (40348595)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | Bebesia gibsoni / アトバコン / 連続培養 / ミトコンドリア / Dehydroorotate dehydrogenase / 多剤併用療法 / Babesia gibsoni |
|---|
| Research Abstract |
The purpose of this study was to establish an effective usage of atovaquone(ATV) against Babesia gibsoni, which is a pathogen of canine Babesiosis. We performed some experiment using B. gibsoni continuous culture ;(1) analysis of mitochondrial function,(2) molecular analysis,(3) sensitivity test using other chemicals,(4) interaction test among ATV and other drugs against B. gibsoni, and(5) assessment of treatment efficacy of Malarone(R) against canine acute B. gibsoni infection. As the result, it was strongly suggested that the ATV resistance of this protozoa may related to M121I on mitochondorial cytochrome b gene, and also that ATV resistant B. gibsoni have not cross resistance against other chemicals. Combination of ATV and other drugs showed synergic to additive efficacy against the protozoal multiplication. In this study, it was also shown that Malarone(R) have high efficacy against acute B. gibsoni infection in dogs.
|
