| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Our objectivewas to investigate the effectsof doxycycline, a matrix metalloproteinase(MMP) inhibitor(MMPi) on ss-agonist-induced myocardial fibrosis and MMP expression. Rats were divided into 3groups : control, Isoproterenol(ISO), and Isoproterenolwith doxycycline(ISO+DOX). Compared to the control, ISO induced the myocardial hypertrophyand cardiacfibrosis, but were attenuated by co-treatment with DOX. MMP-2 activity alsoincreased in the ISO treatment, but decreased by co-treatment with DOX. Similarly, immunoblotting showedsignificant increaseinMMP-2levelin the ISO, and decreased levels in the DOX co-treatment. Ourresults suggest that the enhanced expression of MMP-2plays a prominent role inpromoting myocardial fibrosis inβ-agonist signaling pathway, and that MMP-inhibiting compoundsmay attenuate myocardial fibrosis.
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