| Project/Area Number |
22780288
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Boundary agriculture
|
|---|
| Research Institution | Iwate University |
|---|
Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 植物 / 凍結耐性 / 細胞膜 / 膜修復 / プロテオーム解析 / マイクロドメイン / シナプトタグミン / 細胞膜修復 / 凍結機械ストレス |
|---|
| Research Abstract |
We confirmed that some plants possess the calcium-dependent freezing tolerance, the mechanism of which includes SYT1. The combination of immunoprecipitation and shotgun proteomics showed that the interacting proteins with SYT1 include many plasma membrane microdomain proteins and, only after cold acclimation, tubulin isoforms, actin is forms and RAB. Mutant analyses supported the possibility that VAMP721 and SYP122 are involved in the membrane repair in freezing tolerance mechanism.
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