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Investigation of transformation methods and genes related to coccolith formation, Pleurochrysis carterae

Research Project

Project/Area Number 22780294
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Boundary agriculture
Research InstitutionToyo University

Principal Investigator

NAGASAKA Seiji  東洋大学, 生命科学部, 准教授 (60534013)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsバイオミネラル / 円石藻 / 形質転換 / 生体機能利用 / バイオテクノロジー / 遺伝子
Research Abstract

Coccolithophore is a single-celled algae of the ocean, which forms coccoliths composed mainly of calcium carbonate on the cell surface. Genes, whose amount of expression changes according to the level of calcium concentration in the culture solution, were isolated as ones involved in the coccolith formation. These include the genes which have a high homology with the genes related to polysaccharides biosynthesis. Attempts were made to establish a transformation method for functional analysis of these genes. The injection of genes into cells was successfully conducted, however, their overexpression was not detected.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Morphological and crystallographic transformation from immature to mature coccoliths2011

    • Author(s)
      K. Saruwatari, S. Nagasaka, N. Ozaki, H. Nagasawa.
    • Journal Title

      Pleurochrysis carterae. Marine Biotechnol

      Volume: vol.13 Pages: 801-809

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological and crystallographic transformation from immature to mature coccoliths,Pleurochrysis carterae2011

    • Author(s)
      K. Saruwatari, N. Ozaki, S. Nagasaka and H. Nagasawa
    • Journal Title

      Mar. Biotechnol

      Volume: 13 Issue: 4 Pages: 801-809

    • DOI

      10.1007/s10126-010-9342-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 円石藻のココリスに含まれる新規タンパク質の同定と局在解析2011

    • Author(s)
      笠島大貴, 遠藤博寿, 長坂征治, 横尾直樹, 猿渡和子, 小暮敏博, 藤原祥子, 都筑幹夫, 長澤寛道
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都)
    • Related Report
      2011 Final Research Report
  • [Presentation] 円石藻のココリス形成に関わるタンパク質の探索と解析2010

    • Author(s)
      笠島大貴, 遠藤博寿, 長坂征治, 猿渡和子, 小暮敏博, 長澤寛道
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学(東京)
    • Related Report
      2011 Final Research Report
  • [Book] 分析化学用語辞典2011

    • Author(s)
      社団法人日本分析化学会編
    • Total Pages
      560
    • Publisher
      オーム社
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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