Molecular mechanism of meiotic arrest through Mos-MAPK pathway in mouse oocytes

• Project/Area Number: 22780299
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: KANEMORI Yoshinori 筑波大学, 生命環境系, 助教 (40529088)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 発生 / 分化制御 / マウス / 減数分裂 / シグナル伝達 / MAPキナーゼ / 卵子 / MAPK

Research Abstract

Ovulated oocytes are arrested at the metaphase of second meiotic division (metaphase-II). In this study, we showed possible role of mitogen-and stress-activated kinase 1, MSK1 known as a downstream kinase of the Mos-MAPK pathway, in the mouse oocytes. MSK1 exhibited the ability to phosphorylate four Ser/Thr residues of meiotic cell-cycle regulator EMI2. The phosphorylation was required for up-regulation of the EMI2 activity in the oocytes. These results suggested that mouse MSK1 may play a key role in the metaphase-II arrest through phosphorylation of EMI2.

Research Products

• [Journal Article] (These authors contributed equally) Possible involvement of mitogen-and stress-activated protein kinase 1, MSK1 (2011)
  • Author(s): Miyagaki, Y., Kanemori, Y. and Baba, T
  • Journal Title: in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes. Developmental Biology Volume: 359 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Dynamic regulation of Emi2 by Emi2-bound Cdk1/Plk1/CK1 and PP2A-B56 in meiotic arrest of Xenopus eggs (2011)
  • Author(s): Isoda, M., Sako, K., Suzuki, K., Nishino, K., Nakajo, N., Ohe, M., Ezaki, T., Kanemori, Y., Inoue, D., Ueno, H. and Sagata, N
  • Journal Title: Developmental Cell Volume: 13 Pages: 506-519
  • Peer Reviewed
• [Journal Article] Possible involvement of mitogen- and stress-activated protein kinase 1, MSK1, in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes (2011)
  • Author(s): Yu Miyagaki
  • Journal Title: Developmental Biology Volume: 359(1) Pages: 73-81
  • Peer Reviewed
• [Journal Article] Dynamic regulation of Emi2 by Emi2-bound Cdk1/Plk1/CK1 and PP2A-B56 in meiotic arrest of Xenopus eggs (2011)
  • Author(s): Michitaka Isoda
  • Journal Title: Developmental Cell Volume: 15(21) Pages: 506-519
  • Peer Reviewed
• [Journal Article] Emi2 inhibition of the Anaphase-promoting Complex/Cyclosome absolutely requires Emi2 binding via the C-terminal RL tail (2010)
  • Author(s): Ohe, M., Kawamura, Y., Ueno, H., Inoue, D., Kanemori, Y., Senoo, C., Isoda, M., Nakajo, N. and Sagata, N
  • Journal Title: Molecular Biology of the Cell Volume: 15 Pages: 905-913
  • NAID: 120005227438
  • Peer Reviewed
• [Journal Article] Emi2 inhibition of the anaphase-promoting complex/cyclosomeabsolutely requires Emi2 binding via the C-terminal RL tail. (2010)
  • Author(s): Munemichi Ohe
  • Journal Title: Molecular biology of the cell Volume: 21(6) Pages: 905-913
  • Peer Reviewed
• [Presentation] Direct phosphorylation of EMI2 by MSK1 in the Mos-MAPK pathway during oocyte maturation (2011)
  • Author(s): Miyagaki, Y., Kanemori, Y. and Baba, T
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Direct phosphorylation of EMI2 by MSK1 in the Mos-MAPK pathway during oocyte maturation (2011)
  • Author(s): Yu Miyagaki
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] マウス未受精卵での減数分裂停止の分子メカニズム (2011)
  • Author(s): 兼森芳紀、宮垣佑、馬場忠
  • Organizer: 第52回日本哺乳動物卵子学会
  • Place of Presentation: 大田原
  • Year and Date: 2011-05-21
• [Presentation] マウス未受精卵での減数分裂停止の分子メカニズム (2011)
  • Author(s): 兼森芳紀
  • Organizer: 第52回日本哺乳動物卵子学会
  • Place of Presentation: 国際医療福祉大学(大田原市)
  • Year and Date: 2011-05-21
• [Presentation] Possible involvement of mitogen-and stress-activated protein kinase 1, MSK1 (2011)
  • Author(s): Miyagaki, Y., Kanemori, Y. and Baba, T
  • Organizer: in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes. World Congress on Reproductive Biology & Society for Reproductive Biology
  • Place of Presentation: Cairns (Australia)
• [Presentation] Possible involvement of mitogen- and stress-activated protein kinase 1, MSK1, in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes (2011)
  • Author(s): Yu Miyagaki
  • Organizer: World Congress on Reproductive Biology & Society for Reproductive Biology 2011
  • Place of Presentation: Cairns, Queensland, Australia
• [Presentation] MAPKの下流キナーゼであるMSKのマウス未受精卵での機能解析 (2010)
  • Author(s): 宮垣佑、兼森芳紀、馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] MAPKの下流キナーゼであるMSKのマウス未受精卵での機能解析 (2010)
  • Author(s): 宮垣佑
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸、神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Possible role of mitogen-and stress-activated protein kinase-1 (MSK1) in meiotic arrest of mouse oocytes (2010)
  • Author(s): Miyagaki, Y., Kanemori, Y. and Baba, T.
  • Organizer: Gordon Research Conferences, Reproductive Tract Biology
  • Place of Presentation: USA
• [Presentation] Possible role of mitogen- and stress-activated protein kinase-1 (MSK1) in meiotic arrest of mouse oocytes (2010)
  • Author(s): Yu Miyagaki
  • Organizer: Gordon Research Conferences, Reproductive Tract Biology
  • Place of Presentation: Andover, NH, United States
• [Remarks]
  • URL: http://www.acroman.org/
• [Remarks]
  • URL: http://www.acroman.org/
• [Remarks]
  • URL: http://www.acroman.org/