Physiological analysis of plant mitochondrial fusion and search for genes contributing to the phenomenon

• Project/Area Number: 22780301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: ARIMURA Shin-ichi 東京大学, 大学院・農学生命科学研究科, 准教授 (00396938)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 植物ミトコンドリア / ミトコンドリア融合 / ミトコンドリア分裂 / シロイヌナズナ / ミトコンドリア

Research Abstract

To identify the genes contributing for plant mitochondrial fusion, I screened Arabidopsis mutants with defects in mitochondrial morphology. Three genes of them were newly identified. Furthermore, from results of physiological analysis, I concluded and reported that plant mitochondrial fusion needs ATP.

Research Products

• [Journal Article] Fusion of mitochondria in tobacco suspension cultured cells is dependent on the cellular ATP level but not on actin polymerization (2010)
  • Author(s): Wakamatsu K, Fujimoto M, Nakazono M, Arimura S, Tsutsumi N.
  • Journal Title: Plant Cell Rep Volume: 29(10) Pages: 1139-45
• [Journal Article] Fusion of mitochondria in tobacco suspension cultured cells is dependent on the cellular ATP level but not on actin polymerization. (2010)
  • Author(s): Wakamatsu K, Fujimoto M, Nakazono M, Arimura S, Tsutsumi N.
  • Journal Title: Plant Cell Rep. Volume: 10 Pages: 1139-45
  • Peer Reviewed
• [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell. (2010)
  • Author(s): Takanashi H, Ohnishi T, Mogi M, Okamoto T, Arimura S, Tsutsumi N.
  • Journal Title: Curr Genet. Volume: 56 Pages: 33-41
  • Peer Reviewed
• [Journal Article] 高等植物ミトコンドリアのダイナミクスとその特質 (2010)
  • Author(s): 有村慎一, 山岡尚平, 高梨秀樹, 堤伸浩
  • Journal Title: 細胞工学 Volume: 5月号 Pages: 448-452
• [Presentation] シロイヌナズナミトコンドリア細胞内偏在突然変異体の解析 (2011)
  • Author(s): 有村慎一, 松岡佳奈子, 堤伸浩
  • Organizer: 日本遺伝学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-09-18
• [Presentation] 奨励賞受賞講演高等植物ミトコンドリア分裂・融合現象の解析 (2011)
  • Author(s): 有村慎一
  • Organizer: 日本植物細胞分子生物学会年会
  • Place of Presentation: 福岡県九州大学
  • Year and Date: 2011-09-06
• [Presentation] 新規シロイヌナズナミトコンドリア形態突然変異体の選抜と原因遺伝子同定 (2011)
  • Author(s): 有村慎一,松岡佳奈子,堤伸浩
  • Organizer: 日本植物細胞分子生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2011-09-06
• [Presentation] 高等植物ミトコンドリア分裂・融合現象の解析 (2011)
  • Author(s): 有村慎一
  • Organizer: 日本植物細胞分子生物学会年会
  • Place of Presentation: 福岡県九州大学(招待講演)(奨励賞受賞講演)
  • Year and Date: 2011-09-06
• [Presentation] Domain structure analysis of the Arabidopsis mitochondrial fission protein ELM1 (2011)
  • Author(s): Shin-ichi Arimura, Rina Kurisu, Naoki Kadoya, and Nobuhiro Tsutsumi
  • Organizer: Domain structure analysis of the Arabidopsis mitochondrial fission protein ELM1
  • Place of Presentation: ドイツフルダ
  • Year and Date: 2011-05-15
• [Presentation] Domain structure analysis of the Arabidopsis mitochondrial fission protein ELM1 (2011)
  • Author(s): Shin-ichi Arimura, Rina Kurisu, Naoki Kadoya, Nobuhiro Tsutsumi
  • Organizer: International Congress on Plant Mitochondrial Biology
  • Place of Presentation: ドイツフルダ
  • Year and Date: 2011-05-15
• [Presentation] ライブイメージングを用いた植物ミトコンドリアの分裂・融合・維持機構の解析 (2010)
  • Author(s): 有村慎一
  • Organizer: 京都産業大学
  • Place of Presentation: 京都産業大学
  • Year and Date: 2010-12-10
• [Presentation] Molecular mechanisms underlying the plant mitochondrial fission and fusion (2010)
  • Author(s): 有村慎一・堤伸浩
  • Organizer: 分子生物学会生化学会合同大会ワークショップ"ミトコンドリア研究の最前線:基礎生命科学から臨床まで"
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Molecular mechanisms underlying the plant mitochondrial fission and fusion. (2010)
  • Author(s): 有村慎一・堤伸浩
  • Organizer: BMB 2010
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-09
• [Presentation] ライブイメージングで明らかにする植物ミトコンドリア形態ダイナミクス (2010)
  • Author(s): 有村慎一
  • Organizer: 視る生物学5
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2010-11-25
• [Presentation] 植物ミトコンドリアのダイナミクスを支える分子メカニズム (2010)
  • Author(s): 有村慎一
  • Organizer: 第28回日本植物細胞分子生物学会
  • Place of Presentation: (仙台)大会・シンポジウム
  • Year and Date: 2010-09-02
• [Book] 高等植物ミトコンドリアのダイナミクスとその特質細胞工学 (2010)
  • Author(s): 有村慎一、山岡尚平、高梨秀樹、堤伸浩
• [Remarks] 平成23年度植物細胞分子生物学会奨励賞受賞
• [Remarks] 平成24年度文部科学大臣表彰若手科学者賞受賞