| Project/Area Number |
22780304
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tottori University |
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Principal Investigator |
NAKAYAMA Yuji 鳥取大学, 生命機能研究支援センター, 助教 (40432603)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 遺伝子 / 染色体工学 / ラクダ一本鎖抗体 / ヒト人工染色体 / 抗体多様性産出 / V(D)J組み換え / ADLib法 |
|---|
| Research Abstract |
Camels have single-chain antibody consisting of only heavy chains. Comparing with standard H2L2 antibodies, single-chain antibody have many advantageous chemical features toward antibody-medicine development, such as high solubility in water and/or thermo-stability. In this study we employed chromosomal engineering to produce camel heavy chain antibody in cell culture system. We have successfully cloned the camel chromosome containing whole antibody-gene cluster into cultured cells, however, we could not detect any camel heavy chain antibody in vitro, suggesting incompatibility of camel immune system with others in spite of highly sequence similarity.
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