In vitro production of the Camel heavy chain antibody toward development of alternative antibody medicine

• Project/Area Number: 22780304
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Tottori University
• Principal Investigator: NAKAYAMA Yuji 鳥取大学, 生命機能研究支援センター, 助教 (40432603)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 遺伝子 / 染色体工学 / ラクダ一本鎖抗体 / ヒト人工染色体 / 抗体多様性産出 / V(D)J組み換え / ADLib法

Research Abstract

Camels have single-chain antibody consisting of only heavy chains. Comparing with standard H2L2 antibodies, single-chain antibody have many advantageous chemical features toward antibody-medicine development, such as high solubility in water and/or thermo-stability. In this study we employed chromosomal engineering to produce camel heavy chain antibody in cell culture system. We have successfully cloned the camel chromosome containing whole antibody-gene cluster into cultured cells, however, we could not detect any camel heavy chain antibody in vitro, suggesting incompatibility of camel immune system with others in spite of highly sequence similarity.

Research Products

• [Journal Article] Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells (2012)
  • Author(s): Rozanaliza Radzi, 他(6番目著者)
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 5 Pages: 545-551
  • DOI: 10.1292/jvms.11-0464
  • NAID: 130001410278
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector (2011)
  • Author(s): Yamaguchi S, Kazuki Y, Nakayama Y, Nanba E, Oshimura M, Ohbayashi T
  • Journal Title: PLos One Volume: 6
  • Peer Reviewed
• [Journal Article] Photodynamic Hyperthermal therapy with Indocyanine Green(ICG) induces apoptosis and cell cycle arrest in B16F10 murine melanoma cells (2011)
  • Author(s): Rozanaliza R, Osaki T, Tsuka T, Imagawa T, Minami S, Nakayama Y, Okamoto Y
  • Journal Title: Journal of veterinary medical science Pages: 11-464
  • NAID: 130001410278
  • Peer Reviewed
• [Journal Article] SIRT2 downregulation in HeLa can induce p53 accumulation via p38 MAPK activation-dependent p300 decrease eventually leading to apoptosis (2011)
  • Author(s): Li Y, Matsumori H, Nakayama Y, Osaki M, Kojima H, Kurimasa A, Ito H, Mori S, Katoh M, Oshimura M, Inoue T.
  • Volume: 16
  • Pages: 34-45
  • Peer Reviewed
• [Journal Article] A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector (2011)
  • Author(s): 山口繁之, ほか(3番目著者)
  • Journal Title: PLoS One Volume: 6(2)
  • Peer Reviewed
• [Journal Article] SIRT2 down-regulation in HeLa can induce p53 accumulation via p38 MAPK activation-dependent p300 decrease, eventually leading to apoptosis (2011)
  • Author(s): Li Yanze, 他(3番目著者)
  • Journal Title: Genes to Cells Volume: 16(1) Pages: 34-45
  • Peer Reviewed
• [Journal Article] Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer (2010)
  • Author(s): 加藤基伸, ほか(6番目著者)
  • Journal Title: BMC Biotechnology Volume: 8(8) Pages: 10-37
  • Peer Reviewed
• [Journal Article] SIRT2 downregulation confers resistance to microtubule inhibitors by prolonging chronic mitotic arrest (2009)
  • Author(s): Inoue T, Nakayama Y, Yamada H, Li YC, Yamaguchi S, Osaki M, Kurimasa A, Hiratsuka M, Katoh M, Oshimura M.
  • Journal Title: Cell Cycle Volume: 8 Pages: 1279-1291
  • Peer Reviewed
• [Presentation] 未分化性細胞を用いた脆弱X症候群に見られるCGGトリプレットリピートの伸長メカニズムの解析 (2011)
  • Author(s): 荻野由加利、中山祐二、難波栄二
  • Organizer: 第56回日本人類遺伝学会大会・第11回東アジア人類遺伝学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-11-11
• [Presentation] 特定染色体領域に集積する機能分子複合体の解析 (2011)
  • Author(s): 瀬古朋美、山口繁幸、吉村祐貴、中山祐二、加藤基伸、大林徹也、押村光雄、久郷裕之
  • Organizer: 第56回日本人類遺伝学会大会・第11回東アジア人類遺伝学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-11-11
• [Presentation] Construction of in vitro model system to understand molecular mechanism of CGG repeat expansion in Fragile X syndrome (2011)
  • Author(s): 中山祐二、瀬古朋美、砂村直洋、荻野由加利、加藤基伸、難波栄二、押村光雄、久郷裕之
  • Organizer: 第12回国際人類遺伝学会(ICHG)
  • Place of Presentation: Montrial Convention Center(カナダ、モントリオール)
  • Year and Date: 2011-10-12
• [Presentation] A Novel proteomics approach for idenfitication of protein-RNA complex associated with specific gene loci using a human artificial chromosome (2011)
  • Author(s): 瀬古朋美、山口繁幸、吉村祐貴、中山祐二、加藤基伸、大林徹也、押村光雄、久郷裕之
  • Organizer: 第12回国際人類遺伝学会(ICHG)
  • Place of Presentation: Montrial Convention Center(カナダ、モントリオール)
  • Year and Date: 2011-10-12
• [Presentation] 人工染色体の応用から再生医療まで-鳥取大学における幹細胞アプリケーション- (2011)
  • Author(s): 中山祐二
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: (株)ベックマンコールターランチョンセミナー
  • Year and Date: 2011-06-26
• [Presentation] 人工染色体の応用から再生医療まで-鳥取大学における幹細胞アプリケーション- (2011)
  • Author(s): 中山祐二
  • Organizer: 第21回日本サイトメトリー学会学術集会ベックマンコールターランチョンセミナー
  • Place of Presentation: 京都国際交流会館(京都)(招待講演)
  • Year and Date: 2011-06-26
• [Presentation] 複数の遺伝子搭載が可能な人工染色体ベクターの構築 (2011)
  • Author(s): 荻野由加利、松森はるか、押村光雄、中山祐二、井上敏昭
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] がん治療の新たな標的分子としてのSIRT2 (2011)
  • Author(s): 李艶沢、末松知久、中山祐二、押村光雄、井上敏昭
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] Chromosome transfer via microcell fusion utilizing fusogenic envelope proteins of Measles virus (2010)
  • Author(s): Katoh M, Kazuki Y, Takahashi H, Kazuki K, Kajitani N, Takiguchi M, Nakayama Y, Nakamura T, Inoue T, Oshimura M
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートピアアイランド(神戸市)
  • Year and Date: 2010-12-10
• [Remarks] 鳥取大学生命機能研究支援センター
  • URL: http://grc1.med.tottori-u.ac.jp/Seimei/
• [Remarks] 鳥取大学医学部生命科学科ゲノム医工学分野
  • URL: http://www.med.tottori-u.ac.jp/genome/309/