Development of a novel method for quantitative profiling of non-enzymatic post-translational modifications on abundant serum protein

• Project/Area Number: 22790032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: GOTO Takaaki 東北大学, 大学院・薬学研究科, 講師 (40344684)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 質量分析法 / プロテオミクス / 翻訳後修飾 / 非酵素的翻訳後修飾 / イムノアフィニティー抽出 / MALDI / 血中タンパク質 / 血清アルブミン

Research Abstract

A novel methodology to profile various non-enzymatic post-translational modifications on serum albumin has been examined. This method is based on mass spectrometry combined with the group-specific immunoaffinity extraction, digestion by proteases with distinct specificity, and complementary data analyses in both positive and negative mode. This strategy can be used for profiling analyses of modifications on albumin in human serum.

Research Products

• [Journal Article] Mass spectrometric characterization of modification to angiotensin II by lipid peroxidation products, 4-oxo-2(E)-nonenal and4-hydroxy-2(E)-nonenal (2010)
  • Author(s): Lee, S.H., Takahashi, R., Goto, T., Oe, T.
  • Journal Title: Chem. Res. Toxicol Volume: 23 Issue: 11 Pages: 1771-1785
  • DOI: 10.1021/tx100228q
  • Peer Reviewed
• [Journal Article] Mass spectrometric characterization of modifications to angiotensin II by lipid peroxidation products, 4-oxo-2(E)-nonenal and 4-hydroxy-2(E)-nonenal. (2010)
  • Author(s): Sion Hwa Lee
  • Journal Title: Chem.Res.Toxicol. Volume: 23 Pages: 1771-1785
  • Peer Reviewed
• [Presentation] 血清アルブミンの化学的ストレスによる被修飾部位解析 (2011)
  • Author(s): 村田和之
  • Organizer: 第50回記念日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-30
• [Presentation] 血清アルブミンの化学的ストレスによる被修飾部位解析 (2011)
  • Author(s): 村田和之、後藤貴章、李宣和、大江知行
  • Organizer: 第50回日本薬学会東北支部大会
  • Place of Presentation: 仙台
• [Presentation] バイオマーカー探索を指向したケミカルモディフィコミクスへのアプローチ(依頼講演) (2010)
  • Author(s): 後藤貴章
  • Organizer: 平成22年度日本薬学会東北支部総会・学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-10
• [Presentation] バイオマーカー探索を指向したケミカルモディフィコミクスへのアプローチ (2010)
  • Author(s): 後藤貴章
  • Organizer: 平成22年度日本薬学会東北支部総会・学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-10