A method for simultaneous detection of dual post-translational modifications using phosphate-affinity electrophoresis
| Project/Area Number |
22790037
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KINOSHITA Emiko 広島大学, 大学院・医歯薬学総合研究科, 助教 (40379912)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | リン酸親和性2次元電気泳動法 / Phos-tag SDS-PAGE / 翻訳後修飾 / リン酸化 / ユビキチン化 / βカテニン / 卵白アルブミン / 蛍光ディファレンシャル電気泳動法 / β-カテニン / ナノ材料 / 分子認識 / 蛋白質 / バイオテクノロジー / 分析化学 / フォスタグ / 電気泳動 |
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| Research Abstract |
The researcher developed a method for simultaneous detection of dual post-translational modifications using two-dimensional electrophoresis based on the original principal of phosphate-affinity electrophoresis, Phos-tag SDS-PAGE. By coupling general SDS-PAGE as the first dimension and Phos-tag SDS-PAGE as the second dimension, dual post-translational modifications of cellular proteins, such as phosphorylation and ubiquitination, were detectable simultaneously on the same gel.
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Report
(3 results)
Research Products
(55 results)
