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Development of Bio-hybrid fluorescent probe for detection of cellular microenvironmentally change

Research Project

Project/Area Number 22790048
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKeio University

Principal Investigator

NISHIO Tadashi  慶應義塾大学, 薬学部, 助教 (80401892)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバイオハイブリッド蛍光プローブ / 環境応答型蛍光ポリマー / 細胞内環境変化 / 細胞蛍光イメージング / バイオハイブリッド型蛍光プローブ / 細胞内環境 / イメージング / 環境応答性ポリマー / 温度応答性 / pH応答性
Research Abstract

Our research focuses on the developing dual pH-and temperature-responsive fluorescent probe and applying to the cells for monitoring inside their micro-environment. To construct the novel fluorescent probes, we use the temperature-responsive polymer, which is one of the most representative "intelligent polymer" that exhibits hydrophilic-hydrophobic change in the vicinity of its lower critical solution temperature. In this study, we developed biohybrid fluorescent probe based on the intelligent polymer and investigated their fluorescence change and kinetic change in the cell

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (17 results)

  • [Journal Article] Effect of polymer containing a naphthyl-alanine derivative on the separation selectivity for aromatic compounds in temperature-responsive chromatography2012

    • Author(s)
      Nishio T., Kanazashi R., Nojima A., Kanazawa H., Okano T.
    • Journal Title

      J. Chromatogr. A

      Volume: 1228 Pages: 148-154

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of polymer containing a naphthyl-alanine derivative on the separation selectivity for aromatic compounds in temperature-responsive chromatography2012

    • Author(s)
      Tadashi Nishio, Akari Nojima, Hideko Kanazawa, et al.
    • Journal Title

      J. Chromatogr. A

      Volume: 1228 Pages: 148

    • DOI

      10.1016/j.chroma.2011.07.047

    • URL

      https://pure.teikyo.jp/en/publications/06803ff1-2c01-4868-9443-a55ae019ddeb

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography2011

    • Author(s)
      Nagata Y., Nishio T., Kanazawa H.
    • Journal Title

      J. Pharm. Biomed. Anal.

      Volume: 55 Pages: 241-246

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Separation of phosphorylated peptides utilizing dual pH-and temperature-responsive chromatography2011

    • Author(s)
      Nishio T., Ayano E., Suzuki Y., Kanazawa H., Okano T.
    • Journal Title

      J. Chromatogr. A

      Volume: 1218 Pages: 2079-2084

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography.2011

    • Author(s)
      Nagata Yoshiko
    • Journal Title

      J.Pharm.Biomed.Anal.

      Volume: 55 Pages: 241-246

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 誘導体化LC-MSによる温度応答性クロマトグラフィーにおける高感度化2012

    • Author(s)
      西尾忠
    • Organizer
      日本薬学会132回年会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] アミノ酸誘導体ポリマーの分子間相互作用を利用する温度応答性クロマトグラフィー2011

    • Author(s)
      西尾忠
    • Organizer
      第22回クロマトグラフィー科学会議
    • Place of Presentation
      宮城県,仙台
    • Year and Date
      2011-10-21
    • Related Report
      2011 Final Research Report
  • [Presentation] アミノ酸誘導体ポリマーの分子間相互作用を利用する温度応答性クロマトグラフィー2011

    • Author(s)
      西尾忠
    • Organizer
      第22回クロマトグラフィー科学会議
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 芳香族アミノ酸含有ポリマーによるp-p相互作用を利用した温度応答性クロマトグラフィー2011

    • Author(s)
      西尾忠
    • Organizer
      フィジカルファーマフォーラム(PPF2011)
    • Place of Presentation
      神奈川県,箱根
    • Year and Date
      2011-09-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Aqueous Chromatographic System utilizing Temperature-Responsive Polymer Containing Aromatic Amino Acid2011

    • Author(s)
      Nishio T.
    • Organizer
      36th International Symposium on High Performance Liquid Phase Separations and Related Techniques(HPLC2011)
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2011-06-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Aqueous Chromatographic System utilizing Temperature-Responsive Polymer Containing Aromatic Amino Acid2011

    • Author(s)
      Tadashi Nishio
    • Organizer
      36th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2011)
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2011-06-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 芳香族アミノ酸含有ポリマーを用いる温度応答性クロマトグラフィー2011

    • Author(s)
      西尾忠
    • Organizer
      第18回クロマトグラフィーシンポジウム
    • Place of Presentation
      福岡,福岡県
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Temperature-Responsive Chromatography utilizing Amino-Acids Based Functional Copolymer2011

    • Author(s)
      Nishio T.
    • Organizer
      IUPAC International Congress on Analytical Sciences2011
    • Place of Presentation
      Kyoto
    • Year and Date
      2011-05-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Temperature-Responsive Chromatography utilizing Amino-Acids Based Functional Copolymer2011

    • Author(s)
      Tadashi Nishio
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 芳香族アミノ酸を導入した機能性ポリマーによる温度応答性クロマトグラフィー2011

    • Author(s)
      西尾忠
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡,静岡
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体分子可視化を目指した環境応答型蛍光プローブの開発2010

    • Author(s)
      嶋村美来
    • Organizer
      第26回ライフサポート学会
    • Place of Presentation
      大阪府
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞内環境イメージングを指向したバイオハイブリッド型蛍光プローブ2010

    • Author(s)
      西尾忠
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      宮城県,仙台
    • Year and Date
      2010-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞内環境イメージングを指向したバイオハイブリッド型蛍光プローブ2010

    • Author(s)
      西尾忠
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      宮城県
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 環境応答型ポリマーを用いる細胞イメージング研究2010

    • Author(s)
      西尾忠
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      宮城県,松島
    • Year and Date
      2010-07-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 環境応答型ポリマーを用いる細胞イメージング研究2010

    • Author(s)
      西尾忠
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      宮城県
    • Year and Date
      2010-07-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel Aqueous Chromatographic S ystem for Quantification of Pharmaceutical Co mpounds using Temperature-Responsive Polym ers as the Stationary Phase2010

    • Author(s)
      Nishio T.
    • Organizer
      35th International Symposium on High Performance Liquid Pha se Separations and Related Techniques(HPLC2010)
    • Place of Presentation
      Boston, USA
    • Year and Date
      2010-06-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Aqueous Chromatographic System for Quantification of Pharmaceutical Compounds using Temperature-Responsive Polymers as the Stationary Phase2010

    • Author(s)
      Tadashi Nishio
    • Organizer
      35th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2010
    • Place of Presentation
      Boston, USA
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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