| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Our research focuses on the developing dual pH-and temperature-responsive fluorescent probe and applying to the cells for monitoring inside their micro-environment. To construct the novel fluorescent probes, we use the temperature-responsive polymer, which is one of the most representative "intelligent polymer" that exhibits hydrophilic-hydrophobic change in the vicinity of its lower critical solution temperature. In this study, we developed biohybrid fluorescent probe based on the intelligent polymer and investigated their fluorescence change and kinetic change in the cell
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