Development of Bio-hybrid fluorescent probe for detection of cellular microenvironmentally change
| Project/Area Number |
22790048
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイオハイブリッド蛍光プローブ / 環境応答型蛍光ポリマー / 細胞内環境変化 / 細胞蛍光イメージング / バイオハイブリッド型蛍光プローブ / 細胞内環境 / イメージング / 環境応答性ポリマー / 温度応答性 / pH応答性 |
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| Research Abstract |
Our research focuses on the developing dual pH-and temperature-responsive fluorescent probe and applying to the cells for monitoring inside their micro-environment. To construct the novel fluorescent probes, we use the temperature-responsive polymer, which is one of the most representative "intelligent polymer" that exhibits hydrophilic-hydrophobic change in the vicinity of its lower critical solution temperature. In this study, we developed biohybrid fluorescent probe based on the intelligent polymer and investigated their fluorescence change and kinetic change in the cell
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Report
(3 results)
Research Products
(22 results)
