Occurrence and decomposition behavior of neonicotinoids in food and water environment

Research Project

Project/Area Number	22790050
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Physical pharmacy
Research Institution	Hoshi University
Principal Investigator	KAWAGUCHI Rie 星薬科大学, 薬学部, 助教 (90398892)
Project Period (FY)	2010-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	ネオニコチノイド / 残留分析 / ハチミツ / ネオニコチノイド系農薬 / 分析法構築 / 食品分析 / 環境分析
Research Abstract	In recent years, as an alternative of organophosphate insecticides, neonicotinoids has been widely used. In order to clarify the residue of neonicotinoids in honey, solid phase extraction (SPE) followed by liquid chromatography tandem mass spectrometry was developed for simultaneous determination of neonicotinoids in honey. When ENVI-Carb/LC-NH2 cartridge was used for SPE, the recoveries of neonicotinoids were improved. In addition, the limits of detection (LODs) and limits of qualification (LOQs) of neonicotinoids were 0.002-0.02 ng/mL and 0.005-0.1 ng/mL, respectively. Compared to liquid-liquid extraction or other SPE cartridge, proposed method is sensitive and simple. The results showed that proposed method is applicable to the determination of trace amounts of neonicotinoids in honey.