Study of translational regulation for cell proliferation by polyamines

• Project/Area Number: 22790057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Chiba University
• Principal Investigator: NISHIMURA Kazuhiro 千葉大学, 大学院・薬学研究院, 准教授 (60302569)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ポリアミン / 細胞増殖 / 翻訳 / ハイプシン / ジーントラップ / 転写因子 / RNA / EMSA / 細胞分化 / アクロレイン

Research Abstract

Polyamines (putrescine, spermidine, spermine) have important roles for cell function as a growth factor. In this study, we identified the target molecules translationally regulated by polyamines, because polyamines mainly binds to RNA in cells. Spermidine is required for modification of eukaryotic translation initiation factor 5A (eIF5A). It was clarified that eIF5A and its modification enzyme disruption in mice appeared the embryonic lethality.

Research Products

• [Journal Article] Essential role of eIF5A-1 and deoxyhypusine synthase in mouse embryonic development (2012)
  • Author(s): Nishimura, K., Lee, S. B., Park, J. H., Park, M. H
  • Journal Title: Amino Acids Volume: 42 Pages: 703-710
  • Peer Reviewed
• [Journal Article] Brain infarction correlates more closely with acrolein than with reactive oxygen species (2011)
  • Author(s): Saiki, R., Park, H., Ishii, I., Yoshida, M., Nishimura, K., Toida, T., Tatsukawa, H., Kojima, S., Ikeguchi, Y., Pegg, A. E., Kashiwagi, K., Igarashi, K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 404 Pages: 1044-1049
  • Peer Reviewed
• [Journal Article] Brain infarction correlates more closely with acrolein than with reactive oxygen species (2011)
  • Author(s): Saiki, R., et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 404 Issue: 4 Pages: 1044-1049
  • DOI: 10.1016/j.bbrc.2010.12.107
  • Peer Reviewed
• [Journal Article] Identification and functions amino acid residues in PotB and PotC involved in spermidine uptake activity (2010)
  • Author(s): Higashi, K., Sakamaki, Y., Herai, E., Demizu, R., Uemura, T., Saroj, S. D., Zenda, R., Terui, Y., Nishimura, K., Toida, T., Kashiwagi, K., Igarashi, K
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 39061-39069
  • Peer Reviewed
• [Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction. Biochem (2010)
  • Author(s): Yoshida, M., Higashi, K., Jin, L., Machi, Y., Suzuki, T., Masuda, A., Dohmae, N., Suganami, A., Tamura, Y., Nishimura, K., Toida, T., Tomitori, H., Kashiwagi, K., Igarashi, K
  • Journal Title: Biophys. Res. Commun Volume: 391 Pages: 1234-1239
  • Peer Reviewed
• [Journal Article] Functional significance of eIF5A and its hypusine modification in eukaryotes (2010)
  • Author(s): Park, M. H., Nishimura, K., Zanelli, C. F., Valentini, S. R
  • Journal Title: Amino Acids Volume: 38 Pages: 491-500
  • Peer Reviewed
• [Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction. (2010)
  • Author(s): Yoshida, M., et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 391 Pages: 1234-1239
  • Peer Reviewed
• [Journal Article] Identification and functions amino acid residues in PotB and PotC involved in spermidine uptake activity. (2010)
  • Author(s): Higashi, K., et al
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 39061-39069
  • Peer Reviewed
• [Journal Article] Functional significance of eIF5A and its hypusine modification in eukaryotes. (2010)
  • Author(s): Park, M.H., et al
  • Journal Title: Amino Acids Volume: 38 Pages: 491-500
  • Peer Reviewed
• [Presentation] 天然物由来ウロン酸の分析 (2012)
  • Author(s): 松永成永, 東恭平, 西村和洋, 戸井田敏彦
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-30
• [Presentation] 脳梗塞における新規マーカーとしてのアクロレインによるインターロイキン6及びC反応性タンパク質の産生誘導機構 (2012)
  • Author(s): 幾尾由紀子, 西村和洋, 林大輔, 三村花華, 小林カオル, 千葉寛, 柏木敬子, 東恭平, 戸井田敏彦, 五十嵐一衛
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] 脳梗塞モデルマウス及び培養細胞におけるカルシウム毒性とアクロレインの関連 (2012)
  • Author(s): 斎木遼太, 廣瀬直雄, 朴恵林, 西村和洋, 戸井田敏彦, 山嵜健一, 池口文彦, 白幡晶, 柏木敬子, 五十嵐一衛
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] 脳梗塞患者における尿中アクロレイン-グルタチオン代謝物3-HPMA(3-ヒドロキシプロピルメルカプツール酸)の低下 (2012)
  • Author(s): 吉田円, 三上貴弘, 東恭平, 斎木遼太郎, 溝井睦美, 福田和正, 中村孝雄, 石井伊都子, 西村和洋, 戸井田敏彦, 富取秀行, 柏木敬子, 五十嵐一衛
  • Organizer: 日本ポリアミン学会第3回年会
  • Place of Presentation: 大宮
  • Year and Date: 2012-01-26
• [Presentation] Essential Role of Polyamines and Hypusine in Mouse Embryonic Development (2011)
  • Author(s): Nishimura, K
  • Organizer: 2011 Joint symposium College of Pharmacy, Seoul National University & Graduate School of Pharmaceutical Sciences, ChibaUniversity
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-10-14
• [Presentation] Essential Role of Polyamines and Hypusine in Mouse Embryonic Development (2011)
  • Author(s): Nishimura Kazuhiro
  • Organizer: 2011 Joint symposium College of Pharmacy, Seoul National University & Graduate School of Pharmaceutical Sciences, Chiba University
  • Place of Presentation: ソウル大学(韓国)(招待講演)
  • Year and Date: 2011-10-14
• [Presentation] 細胞培養系及び脳梗塞モデルマウスにおけるCa2+並びにアクロレイン量と細胞毒性の相関 (2011)
  • Author(s): 朴恵林, 斎木遼太郎, 西村和洋, 戸井田敏彦, 山嵜健一, 池口文彦, 白幡晶, 柏木敬子, 五十嵐一衛
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] 脳におけるNMDA受容体の機能-知的能力形成とポリアミン輸送- (2011)
  • Author(s): 吉沢祐基, 東恭平, 富取秀行, Hamdan, F. F., 西村和洋, 戸井田敏彦, 柏木敬子, 五十嵐一衛
  • Organizer: 「ポリアミンと核酸の共進化」第10回合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-09-10
• [Presentation] Effect of acrolein on IL-6 and C-reactive protein production in cultured cells and mouse stroke model (2011)
  • Author(s): Nishimura, K., Ikuo, Y., Hayashi, D., Saiki, R., Park, H., Ishii, I., Mimura, H., Kobayashi, K., Chiba, K., Kashiwagi, K., Toida, T., Igarashi, K
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2011-06-19
• [Presentation] Correlation between calcium ion entry and acrolein production (2011)
  • Author(s): Saiki, R., Park, H., Nishimura, K., Toida, T., Yamazaki, K., Ikeguchi, Y., Shirahata, A., Kashiwagi, K., Igarashi, K
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2011-06-19
• [Presentation] 脳機能改善薬としての機能を目指したメマンチン誘導体の合成とNMDA受容体活性阻害(その2) (2011)
  • Author(s): 熊本卓哉, 中嶋真里恵, 片川和明, 吉沢佑基, 西村和洋, 戸井田敏彦, 五十嵐一衛
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-31
• [Presentation] NMDA受容体調節領域NR1RとNR2BRの構造と機能 (2011)
  • Author(s): 吉沢佑基, 水野聡美, 富取秀行, 韓霞, 益子崇, 菅波晃子, 田村裕, 東恭平, 斎木遼太郎, 西村和洋, 戸井田敏彦, 柏木敬子, 五十嵐一衛
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] スペルミジン輸送活性に関与するPotB及びPotCのアミノ酸残基の同定とその機能 (2011)
  • Author(s): 柏木敬子, 酒巻善春, 東恭平, 戸来江美子, 出水理砂, 植村武史, Sunil D. SAROJ, 照井祐介, 西村和洋, 戸井田敏彦, 五十嵐一衛
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 脳梗塞患者の血漿中に見いだされるアクロレイン抱合蛋白質の分析と同定 (2010)
  • Author(s): 西村和洋, 吉田円, 東恭平, 金麗花, 町佳樹, 鈴木健裕, 益田晶子, 堂前直, 菅波晃子, 田村裕, 戸井田敏彦, 富取秀行, 柏木敬子, 五十嵐一衛
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] アクロレインおよび活性酸素の脳梗塞に果たす役割 (2010)
  • Author(s): 斎木遼太郎, 朴恵林, 石井伊都子, 西村和洋, 戸井田敏彦, 柏木敬子, 五十嵐一衛:
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 天然物由来多糖類に含まれるウロン酸類のHPLCによる分離定量 (2010)
  • Author(s): 松本成永, 東恭平, 西村和洋, 戸井田敏彦
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-17
• [Presentation] 尿中のアクロレイン及びアクロレイン抱合体の定量。「ポリアミンと核酸の共進化」 (2010)
  • Author(s): 三上貴弘, 東恭平, 西村和洋, 吉田円, 戸井田敏彦, 五十嵐一衛
  • Organizer: 第9回合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-09-11
• [Presentation] The story of hypusine and eIF5A : an essential posttranslational modification involving a polyamine (2010)
  • Author(s): Park, M. H., Nishimura, K
  • Organizer: 2010 International Polyamine Conference
  • Place of Presentation: Gotemba, Shizuoka, Japan
  • Year and Date: 2010-06-16
• [Presentation] Generation of eukaryotic translation initiation factor 5A and its post-translational modification enzyme disrupted mice (2010)
  • Author(s): Nishimura, K., Park, M. H
  • Organizer: 2010 International Polyamine Conference
  • Place of Presentation: Gotemba, Shizuoka, Japan
  • Year and Date: 2010-06-15
• [Presentation] More intensive correlation between brain infarction and acrolein than reactive oxygen species (2010)
  • Author(s): Saiki, R., Park, H., Ishii, I., Yoshida, M., Nishimura, K., Toida, T., Tatsukawa, H., Kojima, S., Yamazaki, K., Ikeguchi, Y., Pegg, A. E., Kashiwagi, K., Igarashi, K
  • Organizer: 2010 International Polyamine Conference
  • Place of Presentation: Gotemba, Shizuoka, Japan
  • Year and Date: 2010-06-15
• [Presentation] Generation of eukaryotic translation initiation factor 5A and itspost-translational modification enzyme disrupted mice. (2010)
  • Author(s): Nishimura, K., Park, M.H.
  • Organizer: 2010 International Polyamine Conference
  • Place of Presentation: 御殿場
  • Year and Date: 2010-06-15
• [Remarks]
  • URL: http://www.p.chiba-u.ac.jp/lab/bunseki/