DNA methylation and endothelial cell-specific gene expression
Project/Area Number |
22790066
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Biological pharmacy
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Research Institution | Osaka University |
Principal Investigator |
OKADA Yoshiaki 大阪大学, 大学院・薬学研究科, 助教 (50444500)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | エピジェネティクス / DNAメチル化 / 遺伝子発現制御 / 血管内皮細胞 / Robo4遺伝子 / Robo4 / エビジェネティクス |
Research Abstract |
The CpG sites in the Robo4 proximal promoter is specifically non-methylated in endothelial cells(ECs) but not non-ECs. In this study we performed assays using DNA methyltransferase inhibitor, methylated reporter plasmids, and ES cell lines containing mutant promoters, and demonstrated that cell-type-specific DNA methylation of the 11 CpG sites in the proximal promoter regulates EC-specific Robo4 gene expression.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Embryonic stem cell differentiation system for evaluating gene functions involved in physiological megakaryocytic differentiation2012
Author(s)
Okada Y, Yonekura M, Watanabe M, Nakai T, Wakimura A, Shimizu M, Kamikawa Y, Kitayama M, Kitajima K, Aird WC, Doi T
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Journal Title
Biochem. Biophys. Res. Commun
Volume: Vol.419
Pages: 477-481
Related Report
Peer Reviewed
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[Journal Article] Multiple ETS Family Proteins Regulate PF4 Gene Expression by Binding to the Same ETS Binding Site2011
Author(s)
Okada Y, Nobori H, Shimizu M, Watanabe M, Yonekura M, Nakai T, Kamikawa Y, Wakimura A, Funahashi N, Naruse H, Watanabe A, Yamasaki D, Fukada S, Yasui K, Matsumoto K, Sato T, Kitajima K, Nakano T, Aird WC, Doi T
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] New Protein Purification System Using Gold-Magnetic Beads and a Novel Peptide Tag," the Methionine Tag"2011
Author(s)
Okada Y, Takano TY, Kobayashi N, Hayashi A, Yonekura M, Nishiyama Y, Abe T, Yoshida T, Yamamoto TA, Seino S, Doi T
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Journal Title
Bioconjugate Chemistry
Volume: Vol.22
Pages: 887-893
Related Report
Peer Reviewed
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[Journal Article] Vascular bed specific regulation of the von Willebrand factor promoter in the heart and skeletal muscle2011
Author(s)
Liu J, Yuan L, Molema G, Regan E, Janes L, Beeler D, Spokes KC, Okada Y, Minami T, Oettgen P, Aird WC
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Journal Title
Blood
Volume: Vol.117
Pages: 342-361
Related Report
Peer Reviewed
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