| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Neurotransmitter is stored in synaptic vesicles, is released upon depolarization-evoked stimulation, and binds to specific receptor, which in turn leads to neurotransmission. We identified vesicular excitatory amino acid transporter(VEAT) and vesicular nucleotide transporter(VNUT), which are responsible for vesicular storage of neurotransmitter. In this study, we investigated the physiological significance of aspartergic and purinergic neurotransmission using VEAT and VNUT as molecular probes. We found that(1) VEAT mutants causing Salla disease are devoid of aspartate transport activity,(2) VNUT transports divalent cation complexed with ATP, physiologically,(3) ketone bodies inhibit aspartate, glutamate and ATP release by competing with Cl-,(4) aspartergic neurotransmission is associated with oxidative stress.
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