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The physiological significance of aspartergic and purinergic neurotransmission.

Research Project

Project/Area Number 22790067
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionOkayama University

Principal Investigator

MIYAJI Takaaki  岡山大学, 自然生命科学研究支援センター, 助教 (40550314)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords小胞型ヌクレオチドトランスポーター / 小胞型興奮性アミノ酸トランスポーター / 化学伝達 / サラ病 / 塩素イオン / ケトン体
Research Abstract

Neurotransmitter is stored in synaptic vesicles, is released upon depolarization-evoked stimulation, and binds to specific receptor, which in turn leads to neurotransmission. We identified vesicular excitatory amino acid transporter(VEAT) and vesicular nucleotide transporter(VNUT), which are responsible for vesicular storage of neurotransmitter. In this study, we investigated the physiological significance of aspartergic and purinergic neurotransmission using VEAT and VNUT as molecular probes. We found that(1) VEAT mutants causing Salla disease are devoid of aspartate transport activity,(2) VNUT transports divalent cation complexed with ATP, physiologically,(3) ketone bodies inhibit aspartate, glutamate and ATP release by competing with Cl-,(4) aspartergic neurotransmission is associated with oxidative stress.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010

All Journal Article (22 results) (of which Peer Reviewed: 22 results) Presentation (6 results)

  • [Journal Article] A Na+/phosphate co-transporter homologue(SLC17A4 protein) is an intestinal organic anion exporter2012

    • Author(s)
      Togawa N, Miyaji T, Izawa S, Omote H, Moriyama Y
    • Journal Title

      Am. J. Physiol. Cell Physiol

      Volume: 302巻 Pages: 1652-1650

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Na^+/phosphate co-transporter homologue (SLC17A4 protein) is an intestinal organic anion exporter2012

    • Author(s)
      Togawa N., et al
    • Journal Title

      Am.J.Physiol.Cell Physiol.

      Volume: 302(掲載確定) Pages: 1652-1660

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Divalent cation transport by vesicular nucleotide transporter2011

    • Author(s)
      Miyaji T, Sawada K, Omote H, Moriyama Y
    • Journal Title

      J. Biol. Chem

      Volume: 286巻 Pages: 42881-42887

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin2011

    • Author(s)
      Miyaji T, Omote H, Moriyama Y
    • Journal Title

      J. Neurochem

      Volume: 119巻 Pages: 1-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the human MATE2 proton coupled polyspecific organic cation exporter2011

    • Author(s)
      Komatsu T, Hiasa M, Miyaji T, Kanamoto T, Matsumoto T., Otsuka M, Omote H, Moriyama Y
    • Journal Title

      Int. J. Biochem. Cell Biol

      Volume: 43巻 Pages: 913-918

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vesicular neurotransmitter transporter : Bioenergetics and regulation of glutamate transport2011

    • Author(s)
      Omote H, Miyaji T, Juge N, Moriyama Y
    • Journal Title

      Biochemistry

      Volume: 50巻 Pages: 5558-5565

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 任意のトランスポーターの大量発現法2011

    • Author(s)
      宮地孝明, 森山芳則
    • Journal Title

      生物物理

      Volume: 51巻 Pages: 2-3

    • NAID

      10029482494

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Divalent cation transport by vesicular nucleotide transporter2011

    • Author(s)
      Miyaji T., et al
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 42881-87

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin2011

    • Author(s)
      Miyaji T, Omote H, Moriyama Y
    • Journal Title

      J Neurochem

      Volume: 119 Issue: 1 Pages: 1-5

    • DOI

      10.1111/j.1471-4159.2011.07388.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the human MATE2 proton coupled polyspecific organic cation exporter2011

    • Author(s)
      Komatsu T., et al
    • Journal Title

      Int.J.Biochem.Cell Biol.

      Volume: 43 Pages: 913-918

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vesicular neurotransmitter transporter : bioenergetics and regulation of glutamate transport2011

    • Author(s)
      Omote H., et al
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 5558-5565

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 任意のトランスポーターの大量発現法2011

    • Author(s)
      宮地孝明, et al
    • Journal Title

      生物物理

      Volume: 51 Pages: 2-3

    • NAID

      10029482494

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新たに同定された小胞型神経伝達物質トランスポーター2011

    • Author(s)
      宮地孝明, et al
    • Journal Title

      脳21

      Volume: 14 Pages: 305-310

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 小胞型グルタミン酸トランスポーターの機能と制御2011

    • Author(s)
      表弘志, et al
    • Journal Title

      脳21

      Volume: 14 Pages: 311-314

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic control of vesicular glutamate transport and release2010

    • Author(s)
      Juge N, Gray JA, Omote H, Miyaji T, Inoue T, Hara C, Uneyama H, Edwards RH, Nicoll RA, Moriyama Y
    • Journal Title

      Neuron

      Volume: 68巻 Pages: 99-112

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Type 1 sodium-dependent phosphate transporter(SLC17A1 Protein) is a Cl-dependent urate exporter2010

    • Author(s)
      Iharada M, Miyaji T, Fujimoto T, Hiasa M, Anzai N, Omote H, Moriyama Y
    • Journal Title

      J. Biol. Chem

      Volume: 285巻 Pages: 26107-26113

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The ABC transporter AtABCG25 is involved in abscisic acid transport and responses2010

    • Author(s)
      Kuromori T, Miyaji T, Yabuuchi H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107巻 Pages: 2361-2366

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A vesicular transporter that mediates aspartate and glutamate neurotransmission2010

    • Author(s)
      Miyaji T, Omote H, Moriyama Y
    • Journal Title

      Biol. Pharm. Bull

      Volume: 33巻 Pages: 1783-1785

    • NAID

      130000402295

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic control of vesicular glutamate transport and release.2010

    • Author(s)
      Juge N, et al.
    • Journal Title

      Neuron

      Volume: 68 Pages: 99-112

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Type 1 sodium-dependent phosphate transporter (SLC17A1 Protein) is a Cl- dependent urate exporter.2010

    • Author(s)
      Iharada M, et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 26107-26113

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ABC transporter AtABCG25 is involved in abscisic acid transport and responses.2010

    • Author(s)
      Kuromori T, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 2361-2366

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A vesicular transporter that mediates aspartate and glutamate neurotransmission.2010

    • Author(s)
      Miyaji T, et al.
    • Journal Title

      Biol.Pharm.Rull.

      Volume: 33 Pages: 1783-1785

    • NAID

      130000402295

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] プリン性化学伝達における小胞型ヌクレオチドトランスポーターの意義2011

    • Author(s)
      宮地孝明
    • Organizer
      第38回岡山脳研究セミナー
    • Place of Presentation
      岡山(招待講演)
    • Year and Date
      2011-06-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] プリン性化学伝達における小胞型ヌクレオチドトランスポーターの意義2011

    • Author(s)
      宮地孝明
    • Organizer
      第38回岡山脳研究セミナー
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] ショウジョウバエ小胞型興奮性アミノ酸トランスポーターの機能解析2010

    • Author(s)
      宮地孝明, et al.
    • Organizer
      Neuro2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] ショウジョウバエ小胞型興奮性アミノ酸トランスポーターの機能解析2010

    • Author(s)
      宮地孝明, et al
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターの機能解析2010

    • Author(s)
      宮地孝明
    • Organizer
      第5回トランスポーター研究会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ショウジョウバエ小胞型興奮性アミノ酸トランスポーターの機能解析2010

    • Author(s)
      宮地孝明, et al
    • Organizer
      第32回生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      富山
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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