Molecular mechanisms of permeability transition revealed using yeast mitochondria

• Project/Area Number: 22790070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: YAMAMOTO Takenori 徳島大学, 疾患ゲノム研究センター, 助教 (80457324)
• Research Collaborator: SHINOHARA Yasuo 徳島大学, 疾患ゲノム研究センター, 教授 (60226157)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞生物学 / 透過性遷移 / ミトコンドリア / 酵母 / アポトーシス / 遺伝子破壊 / 遺伝子壊変

Research Abstract

Mitochondrial permeability transition triggers subsequent steps of apoptosis. To understand the molecular mechanisms of permeability transition, we in this research accessed to identify the regulatory genes for permeability transition using yeast mitochondria. As a result, we revealed that possible regulatory genes involved in permeability transition in previous reports did not mainly regulate permeability transition.

Research Products

• [Journal Article] Comparison of two expression systems using COS7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1 (CPT1b) (2012)
  • Author(s): Hada T, Kato Y, Obana E, Yamamoto A, Yamazaki N, Hashimoto M, Yamamoto T, Shinohara Y
  • Journal Title: Protein Expr. Purif Volume: vol.82 Issue: 1 Pages: 192-196
  • DOI: 10.1016/j.pep.2012.01.006
  • Peer Reviewed
• [Journal Article] Pseudogenes of rat VDAC1:16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues (2012)
  • Author(s): Yamamoto, et al
  • Journal Title: Mamm Genome Volume: 23 Issue: 3-4 Pages: 286-293
  • DOI: 10.1007/s00335-011-9375-x
  • Peer Reviewed
• [Journal Article] Pseudogenes of rat VDAC1 : 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues (2011)
  • Author(s): Ido Y, Yamamoto T, Yoshitomi T, Yamamoto A, Obana E, Ohkura K, Shinohara Y
  • Journal Title: Mamm Genome Volume: (in press)
  • Peer Reviewed
• [Journal Article] Properties of signal intensities observed with individual probes of GeneChip Rat Gene 1. 0 ST Array, an affymetric microarray system (2011)
  • Author(s): Obana E, Hada T, Yamamoto T, Kakuhata R, Saze T, Miyoshi H, Hori T, Shinohara Y
  • Journal Title: Biotechnol Lett Volume: 4 Issue: 2 Pages: 213-219
  • DOI: 10.1007/s10529-011-0776-4
  • Peer Reviewed
• [Journal Article] S-15176 and its methylated derivative suppress the CsA-insensitive mitochondrial permeability transition and subsequent cytochrome crelease induced by silver ion, and show weak protonophoric activity (2011)
  • Author(s): Kawashima S, Yamamoto T, Horiuchi Y, Fujiwara K, Gouda S, Yoshimura Y, Yamamoto A, Inotani Y, Yamashita K, Kitamura S, Terada H, Kanematsu M, Shishido K, Shinohara Y
  • Journal Title: Mol Cell Biochem Volume: 358(1-2) Issue: 1-2 Pages: 45-51
  • DOI: 10.1007/s11010-011-0919-x
  • Peer Reviewed
• [Journal Article] Differential effects of cold exposure on gene expression profiles in white versus brown adipose tissue (2011)
  • Author(s): Watanabe M, Yamamoto T, Yamamoto A, Obana E, Niiyama K, Hada T, Ooie T, Kataoka M, Hori T, Houchi H, Shinohara Y
  • Journal Title: Appl Biochem Biotechnol Volume: 165(2) Issue: 2 Pages: 538-547
  • DOI: 10.1007/s12010-011-9273-4
  • Peer Reviewed
• [Journal Article] Identification of TMEM45B as a protein clearly showing thermal aggregation in SDS-PAGE gels and dissection of its amino acid sequence responsible for this aggregation (2011)
  • Author(s): Okada N, Yamamoto T, Watanabe M, Yoshimura Y, Obana E, Yamazaki N, Kawazoe K, Shinohara Y, Minakuchi K
  • Journal Title: Protein Expr Purif Volume: 77(1) Issue: 1 Pages: 118-123
  • DOI: 10.1016/j.pep.2011.01.011
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of the effects of cold exposure of rats on the expression levels of ten FABP isoforms in brown adipose tissue (2011)
  • Author(s): Yamamoto T, Yamamoto A, Watanabe M, Kataoka M, Terada H, Shinohara Y
  • Journal Title: Biotechnol Lett Volume: 33(2) Issue: 2 Pages: 237-242
  • DOI: 10.1007/s10529-010-0444-0
  • Peer Reviewed
• [Journal Article] Use of highly purified and mixed antibodies for simultaneous detection of multiple protein species released from mitochondria upon induction of the permeability transition (2011)
  • Author(s): Yamamoto T, Ohashi M, Mizutani S, Yoshimura Y, Obana E, Terada H, Shinohara Y
  • Journal Title: Appl Biochem Biotechnol Volume: 163(1) Issue: 1 Pages: 64-70
  • DOI: 10.1007/s12010-010-9016-y
  • Peer Reviewed
• [Journal Article] Use of highly purified and mixed antibodies for simultaneous detection of multiple protein species released from mitochondria upon induction of the permeability transition (2011)
  • Author(s): Yamamoto T., et al.
  • Journal Title: Appl Biochem Biotechnol Volume: 163 Pages: 64-70
  • Peer Reviewed
• [Journal Article] Specific formation of trypsin-resistant micelles on a hydrophobic peptide observed with Triton X-100 but not with octylglucoside (2010)
  • Author(s): Katsuda C, Niiyama K, Obana E, Yamamoto T, Katou Y, Kataoka M, Ohkura K, Shinohara Y
  • Journal Title: Biochim Biophys Acta Volume: 1798 Issue: 11 Pages: 2090-2093
  • DOI: 10.1016/j.bbamem.2010.07.013
  • Peer Reviewed
• [Journal Article] Analysis of DNA ligation by microchip electrophoresis (2010)
  • Author(s): Umemoto Y, Kataoka M, Yatsushiro S, Yamamura S, Ooie T, Kido J, Yamamoto T, Shinohara Y, Baba Y
  • Journal Title: J Pharm Biomed Anal Volume: 52 Issue: 2 Pages: 323-328
  • DOI: 10.1016/j.jpba.2009.12.023
  • Peer Reviewed
• [Journal Article] Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1alpha (2010)
  • Author(s): Bando M, Hiroshima Y, Kataoka M, Herzberg MC, Ross KF, Shinohara Y, Yamamoto T, Nagata T, Kido J
  • Journal Title: Immunol Cell Biol Volume: 88 Issue: 3 Pages: 328-333
  • DOI: 10.1038/icb.2009.104
  • Peer Reviewed
• [Journal Article] Replacement of C305 in heart/ muscle-type isozyme of human carnitine palmitoyltransferase I with aspartic acid and other amino acids (2010)
  • Author(s): Matsuo T, Yamamoto A, Yamamoto T, Otsuki K, Yamazaki N, Kataoka M, Terada H, Shinohara Y
  • Journal Title: Biochem Genet Volume: 48 Issue: 3-4 Pages: 193-201
  • DOI: 10.1007/s10528-009-9301-z
  • Peer Reviewed
• [Journal Article] プロテオミクスで探るミトコンドリアからのシトクロムc漏出機構 (2010)
  • Author(s): 山本武範, 山田安希子, 篠原康雄
  • Journal Title: 生物物理 Volume: 50 Pages: 124-125
  • NAID: 10026476927
  • Peer Reviewed
• [Presentation] プロテオミクスで解き明かすミトコンドリアからのシトクロムc放出機構 (2012)
  • Author(s): 山本武範
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] プロテオミクスで解き明かすミトコンドリアからのシトクロムc放出機構 (2012)
  • Author(s): 山本武範
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市(北海道大学)
  • Year and Date: 2012-03-29
• [Presentation] 質量分析法と抗原抗体反応を組み合わせたミトコンドリアタンパク質の分離分析 (2012)
  • Author(s): 山本武範
  • Organizer: 第16回徳島地区分析技術セミナー「分離分析の最新動向」
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2012-01-20
• [Presentation] 質量分析法と抗原抗体反応を組み合わせたミトコンドリアタンパク質の分離分析 (2012)
  • Author(s): 山本武範
  • Organizer: 第16回徳島地区分析技術セミナー「分離分析の最新動向」
  • Place of Presentation: 徳島市(徳島大学)
  • Year and Date: 2012-01-20
• [Presentation] ミトコンドリアからのシトクロムc放出機構解明に向けた多面的アプローチ (2011)
  • Author(s): 山本武範
  • Organizer: 若手研究者公開特別シンポジウム「ミトコンドリアとDDS1」
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-01-21
• [Presentation] ミトコンドリアからのシトクロムc放出機構解明に向けた多面的アプローチ (2011)
  • Author(s): 山本武範, 他
  • Organizer: ミトコンドリアとDDS1
  • Place of Presentation: 北海道大学薬学研究院(札幌市)
  • Year and Date: 2011-01-21
• [Presentation] 褐色脂肪組織におけるEABPアイソフォームの発現レベルに対して寒冷曝露がもたらす作用の定量的評価 (2010)
  • Author(s): 山本武範, 他
  • Organizer: 第32回 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山国際会議場(富山市)
  • Year and Date: 2010-11-29
• [Presentation] 褐色脂肪組織におけるFABPアイソフォームの発現レベルに対して寒冷曝露がもたらす作用の定量的評価 (2010)
  • Author(s): 山本武範、山本篤司、渡邊政博、篠原康雄
  • Organizer: 第32回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山国際会議場(富山市)
  • Year and Date: 2010-11-28
• [Presentation] Ca^<2+>によって酵母ミトコンドリアに誘起される透過性遷移の解析 (2010)
  • Author(s): 山本武範, 他
  • Organizer: 第2回 日本生物物理学会 中国四国支部例会
  • Place of Presentation: 松山大学薬学部(松山市)
  • Year and Date: 2010-05-08