Functional analyses of xylose-containing glycans in the nervous system

• Project/Area Number: 22790076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: YAGI Hirokazu 名古屋市立大学, 大学院・薬学研究科, 助教 (70565423)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糖鎖 / キシロース含有糖鎖 / キシロース転移酵素 / 神経系

Research Abstract

This study aims to elucidate the function of xylosyl-containing glycans. We found that the candidate gene of xylosetransferase(AGO61)-knockout mice displayed embryonic growth retardation, especially brain development at embryonic day 11.5.The present study indicated that the xylosyl-containing glycans play important roles in the development of the nervous system.

Research Products

• [Journal Article] Bovine milk whey for preparation of natural N-glycans : structural and quantitative analysis (2012)
  • Author(s): Sriwilaijaroen, N., Kondo, S., Yagi, H., Hiramatsu, H., Nakakita, S., Yamada, K., Ito, H., Hirabayashi, J., Narimatsu, H., Kato, K. and Suzuki, Y.
  • Journal Title: Open Glycoscience Volume: 5 Pages: 41-50
  • Peer Reviewed
• [Journal Article] Comparative analysis of N-glycosylation profiles of influenza A viruses grown in different host cells (2012)
  • Author(s): Yagi, H., Watanabe, S., Suzuki, T., Takahashi, T., Suzuki, Y. and Kato, K.
  • Journal Title: Open Glycoscience Volume: 5 Pages: 2-12
  • Peer Reviewed
• [Journal Article] Bovine milk whey for preparation of natural N-glycans : structural and quantitative analysis (2012)
  • Author(s): Sriwilaijaroen, N., Kondo, S., Yagi, H., Hiramatsu, H., Nakakita, S., Yamada, K., Ito, H., Hirabayashi, J., Narimatsu, H., Kato, K., Suzuki, Y.
  • Journal Title: Open Glycoscience Volume: 5 Issue: 1 Pages: 2-12
  • DOI: 10.2174/1875398101205010002
  • Peer Reviewed
• [Journal Article] Development and application of multidimensional HPLC mapping method for O-linked oligosaccharides (2011)
  • Author(s): Yagi, H., Ohno, E., Kondo, S., Yoshida, A. and K. Kato
  • Journal Title: Biomolecules Volume: 1 Pages: 48-62
  • Peer Reviewed
• [Journal Article] N-Glycans from porcine trachea and lung : Predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor (2011)
  • Author(s): Sriwilaijaroen, N., Kondo, S., Yagi, H., Takemae, N., Saito, T., Hiramatsu, H., Kato, K. and Suzuki, Y.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Structural and molecular basis of carbohydrate-protein interaction systems as potential therapeutic targets (2011)
  • Author(s): Kamiya, Y., Yagi-Utsumi, M., Yagi, H., Kato, K.
  • Journal Title: Curr. Pharm. Des Volume: 17 Pages: 1672-1684
  • Peer Reviewed
• [Journal Article] N-glycans from porcine trachea and lung : Predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor (2011)
  • Author(s): N. Sriwilaijaroen, S. Kondo, H. Yagi, N. T. T. Saito, H. Hiramatsu, K. Kato, Y. Suzuki
  • Journal Title: PLoS ONE Volume: 6 Issue: 2 Pages: e16302-e16302
  • DOI: 10.1371/journal.pone.0016302
  • Peer Reviewed
• [Journal Article] Structural and molecular basis of carbohydrate?protein interaction systems as potential therapeutic targets. (2011)
  • Author(s): Y.Kamiya, M.Yagi-Utsumi, H.Yagi, and K.Kato
  • Journal Title: Curr. Pharm. Des. Volume: 17 Issue: 17 Pages: 1672-1684
  • DOI: 10.2174/138161211796355074
  • Peer Reviewed
• [Journal Article] HNK-1 epitope-carrying Tenascin-C spliced variant regulates the proliferation of mouse embryonic neural stem cells (2010)
  • Author(s): Yagi, H, Yanagisawa, M., Suzuki, Y., Nakatani, Y., Ariga, T., Kato, K., Yu, R. K.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 37293-37301
  • Peer Reviewed
• [Journal Article] HNK-1 epitope-carrying Tenascin-C spliced variant regulates the proliferation of mouse embryonic neural stem cells (2010)
  • Author(s): Hirokazu Yagi, Makoto Yanagisawa, Yusuke Suzuki, Yoshihiko Nakatani, Toshio Ariga, Koichi Kato, Robert K.Yu
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 37293-37301
  • Peer Reviewed
• [Presentation] 神経幹細胞に発現しているLewisX型糖鎖はNotchシグナル経路を介して幹細胞性の維持に関与している (2012)
  • Author(s): 矢木宏和, 齋藤拓也, 柳澤亮, Robert Yu, 加藤晃一
  • Organizer: 日本薬学会第132年会
  • Year and Date: 2012-03-29
• [Presentation] Identification of specific N-glycans expressed on the nervous systems by HPLC mapping method (2012)
  • Author(s): Hirokazu Yagi and Koichi Kato
  • Organizer: GCOE International Symposium
  • Year and Date: 2012-01-11
• [Presentation] Identification of specific N-glycans expressed on the nervous systems by HPLC mapping method (2012)
  • Author(s): Hirokazu Yagi, Koichi Kato
  • Organizer: GCOE International Symposium
  • Place of Presentation: 名古屋
  • Year and Date: 2012-01-11
• [Presentation] Lewis X containing glycans expressed on mouse embryonic neural stem cells regulates their proliferation via Notch signaling pathway (2011)
  • Author(s): Hirokazu Yagi, Takuya Saito, Makoto Yanagisawa, Robert K. Yu and Koichi Kato
  • Organizer: 第71回岡崎コンファレンス「糖鎖分子科学の新たな展望
  • Year and Date: 2011-10-13
• [Presentation] Lewis X containing glycans expressed on mouse embryonic neural stem cells regulates their proliferation via Notch signaling pathway (2011)
  • Author(s): Hirokazu Yagi, Takuya Saito, Makoto Yanagisawa, Robert K.Yu, Koichi Kato
  • Organizer: 第71回岡崎コンファレンス「糖鎖分子科学の新たな展望
  • Place of Presentation: 岡崎
  • Year and Date: 2011-10-13
• [Presentation] LewisX型糖鎖の神経細胞系における特異的な発現とその機能解析 (2011)
  • Author(s): 齋藤拓也, 矢木宏和, 柳澤亮, Robert K. Yu, 加藤晃一
  • Organizer: 第84回日本生化学会大会
  • Year and Date: 2011-09-22
• [Presentation] The functional significance of the N-glycans in the differentiation of neural stem cells (2011)
  • Author(s): 矢木宏和
  • Organizer: 第34回日本神経科学大会
  • Year and Date: 2011-09-17
• [Presentation] Development of multi-dimensional HPLC mapping for N-glycans and its application for functional glycomics (2011)
  • Author(s): Hirokazu Yagi, Noriko Takahashi, and Koichi Kato
  • Organizer: 98^<th> Session of the Indian Science Congress
  • Year and Date: 2011-01-04
• [Presentation] Development of multi-dimensional HPLC mapping for N-glycans and its application for functional glycomics (2011)
  • Author(s): Hirokazu Yagi, Noriko Takahashi, Koichi Kato
  • Organizer: 98^<th> Session of the Indian Science Congress
  • Place of Presentation: SRM University(Chennai, India)
  • Year and Date: 2011-01-04
• [Presentation] LewisX型糖鎖の神経細胞系における特異的な発現とその機能解析 (2011)
  • Author(s): 齋藤拓也, 矢木宏和, 柳澤亮, Robert K.Yu, 加藤晃一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都(口頭)(ポスター)
• [Presentation] 神経幹細胞における特異的糖鎖の発現と機能 (2010)
  • Author(s): 矢木宏和
  • Organizer: 2010年バイオインフォマティックス研究会
  • Year and Date: 2010-12-28
• [Presentation] HNK-1 glyco-epitopes carried by the largest Tenascin-C spliced variant regulate proliferation of mouse embryonic neural stem cell (2010)
  • Author(s): Hirokazu Yagi, Makoto Yanagisawa, Koichi Kato, Robert K. Yu
  • Organizer: 第28回内藤カンファレンス
  • Year and Date: 2010-07-28
• [Presentation] HNK-1 glyco-epitopes carried by the largest Tenascin-C spliced variant regulate proliferation of mouse embryonic neural stem cell (2010)
  • Author(s): Hirokazu Yagi, Makoto Yanagisawa, Koichi Kato, Robert K.Yu
  • Organizer: 第28回内藤カンファレンス
  • Place of Presentation: 湘南国際センター(神奈川)
  • Year and Date: 2010-07-28
• [Book] バイオ医薬品開発における糖鎖技術,(早川堯夫,掛樋一晃,平林淳監修) (2011)
  • Author(s): 矢木宏和, 加藤晃一
  • Publisher: シーエムシー出版
• [Book] バイオ医薬品開発における糖鎖技術(早川堯夫,掛樋一晃,平林淳監修) (2011)
  • Author(s): 矢木宏和, 加藤晃一
  • Total Pages: 10
  • Publisher: シーエムシー出版
• [Remarks] ホームページ等名古屋市立大学大学院薬学研究科生命分子構造学分野
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/sbk/