| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Research Abstract |
The evolutionarily conserved Wnt/β-catenin pathway is crucial for normal embryogenesis as well as abnormal activation observed in tumors such as colorectal cancers. We identified a low molecular weight compound IMU14 and its derivatives as novel Wnt/ β-catenin pathway inhibitors through an unconventionalvertebrate in vivo screening system, in which effects of test compounds on zebrafish embryogenesis was evaluated. In this study, we aimed to elucidate the action mechanism of the IMU compounds. An immobilized IMU compound co-precipitated a protein complex containing β-catenin and GSK-3β. Furthermore, chemical genetics showed functional interaction between a target molecule of the IMU compounds and GSK-3β.
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