| Project/Area Number |
22790083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
MATSUO Yuri 北里大学, 薬学部, 講師 (10306657)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 神経生物学 / 脳虚血 / プロスタグランジンE_2 / プロスタグランジンE合成酵素 / 好中球 / 炎症 / ノックアウトマウス / 神経細胞死 / 興奮毒性 / プロスタグランジンE2 / 血液脳関門 |
|---|
| Research Abstract |
Recently, we have found that microsomal PGE synthase(mPGES)-1 plays a crucial role in ischemic brain injury. mPGES-1 was expressed not only in neurons and microglia, but also in infiltrated neutrophils. Thus, we examined the role of neutrophlic mPGES-1 in exaggeration of brain ischemia. The number of infiltrated neutrophils observed in infarct regionof mPGES-1 KO mice was significantly less than that of WT mice. In thioglycollate-induced peritoneal neutrophils, the migrations induced by fMLP and IL-8 in mPGES-1 KO neutrophils were significantly lower than those in WT neutrophils. The neurotoxicity induced by glutamate was enhanced by co-culture with WT neutrophils, but not with mPGES-1 KO neutrophils. These results suggest that neutrophilic mPGES-1 contributes to ischemic brain injury by enhancing neutrophilic migration and neuronal excitotoxicity.
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