Regulatory mechanism of SIGIRR/TIR8 expressionn LPS-mediated signaling.

• Project/Area Number: 22790100
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Sojo University
• Principal Investigator: SHUTO Keiko 崇城大学, 薬学部, 助教 (70510692)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: SIGIRR/TIR8 / 炎症制御 / TLRシグナル制御 / p38MAPキナーゼ / 嚢胞性線維症 / LPS / Sp1

Research Abstract

SIGIRR/TIR8 iscrucial for negative regulationToLfRs andIL-1R- mediated inflammatory signaling.Here, we confirmed the expression ofSIGIRR/TIR8 during LPS stimulation in innate immune ce,llsincludingneutrophilic-differentiated and monocytic celAls.a resultsS,IGIRR/TIR8 gene andprotein expression were do-wrnegulated byLPS in both cell lines and primary ce.llsFurthermore, Our observations indicated that-TpL3R84signal is critical forSIGIRR/TIR8 down-regulation. Finally, we clarifiedthat Sp1 is a key factor thatdirectly binds to the proximal promoter of/TSIGRI8RRgene and, consequently,regulates basal SIGIR/RTIR8 expression, which is negatively regulated byLPS-dependent TLR4-p38 pathway.

Research Products

• [Presentation] LPS刺激下の単球・好中球におけるIgおよびTIRドメイン含有受容体SIGIRRの発現および機能解析 (2012)
  • Author(s): 首藤恵子,首藤剛,佐藤美希,佐藤圭創,MaryAnnSuico,内田友二,徳冨直史,甲斐広文
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡(福岡)
  • Year and Date: 2012-12-16
• [Presentation] SIGIRR expression by suppressing Sp1 via -pT3L8R4MAP kinase pathway in monocytes and neutrophils (2012)
  • Author(s): Keiko Uen-oShuto, Tsuyoshi Shuto, Miki Sato, Keizo Sato, Mary Ann Suico, Yuji Uchida, Naofumi Tokutomi, and Hirofumi Kai Lipopolysaccharide decreases
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡(福岡)
  • Year and Date: 2012-12-12
• [Presentation] 変異CFTR発現気道上皮細胞におけるTLR2/4依存的IL-8発現誘導作用に対するIL-17Aの作用 (2010)
  • Author(s): 首藤恵子
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: ハウステンボスユトレヒト会議室
  • Year and Date: 2010-09-10
• [Presentation] Lipopolysaccharide decreases SIGIRR expression by suppressing Sp1 via TLR4-p38 MAP kinase pathway in monocytes and neutrophils
  • Author(s): 首藤恵子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡 (福岡県)
• [Presentation] LPS刺激下の単球・好中球におけるIgおよびTIRドメイン含有受容体SIGIRRの発現および機能解析
  • Author(s): 首藤恵子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡 (福岡県)