Role of 14-3-3 family in regulation of cell cycle progression

• Project/Area Number: 22790103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: KASAHARA Kousuke 愛知県がんセンター(研究所), 発がん制御研究部, 研究員 (90455535)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 分子生物学 / 細胞周期 / DNA障害チェックポイント / 細胞分裂期 / リン酸化シグナル / 14-3-3 / Chk1 / Plk1 / タンパク質リン酸化 / プロテインキナーゼ / Polo-like kinase / 分裂期

Research Abstract

To identify a novel phosphorylation signal in cell cycle progression, we searched for proteins which bound to 14-3-3 family proteins in a cell cycle-dependent manner. As a result, we found 14-3-3 participated in DNA damage checkpoint and mitotic progression through regulation of Chk1 kinase and Plk1 kinase, respectively.

Research Products

• [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition (2013)
  • Author(s): Kousuke Kasahara
  • Journal Title: Nature Communications Volume: -
  • Peer Reviewed
• [Journal Article] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation (2012)
  • Author(s): Li, P., Goto, H., Kasahara, K., Matsuyama, M., Wang, Z., Yatabe, Y., Kiyono, T., Inagaki, M.
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 8 Pages: 1582-1592
  • DOI: 10.1091/mbc.e11-10-0883
  • Peer Reviewed
• [Journal Article] Nuclear Chk1 prevents premature mitotic entry. (2011)
  • Author(s): P. Li, H. Goto, K. Kasahara, M. Matsuyama, Z. Wang, Y. Yatabe, T. Kiyono, M. Inagaki
  • Journal Title: J. Cell Sci. Volume: 124 Pages: 2113-2119
  • Peer Reviewed
• [Journal Article] Nuclear Chk1 prevents premature mitotic entry. (2011)
  • Author(s): Kousuke Kasahara, et al.
  • Journal Title: The Journal of Cell Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] 14-3-3 gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage. (2010)
  • Author(s): K. Kasahara, H. Goto, M. Enomoto, Y. Tomono, T. Kiyono, M. Inagaki
  • Journal Title: EMBO J. Volume: 29 Pages: 2802-2812
  • Peer Reviewed
• [Journal Article] Withaferin A targets intermediate filaments GFAP and vimentin in A model of retinal gliosis. (2010)
  • Author(s): P. Bargagna-Mohan, R.R. Paranthan, A. Hamza, N. Dimova, B. Trucchi, C. Srinivasan, G.I. Elliott, C.G. Zhan, D.L. Lau, H. Zhu, K. Kasahara, M. Inagaki, F. Cambi, R. Mohan
  • Journal Title: J Biol Chem. Volume: 285 Pages: 7657-7669
  • Peer Reviewed
• [Journal Article] 14-3-3 gamma mediates Cdc25A proteolysis to block premature mitoticentry after DNA damage. (2010)
  • Author(s): Kousuke Kasahara, et al.
  • Journal Title: The EMBO Journal Volume: 29 Pages: 2802-2812
  • Peer Reviewed
• [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3gamma and is required fo r metaphase-anaphase transition.
  • Author(s): K. Kasahara, H. Goto, T. Kiyono, N. Watanabe, S. Elowe, E.A. Nigg, M. Inagaki
  • Journal Title: Nat. Commn. Volume: (in press)
  • Peer Reviewed
• [Presentation] Novel mitotic signalling crosstalk between PI3K-Akt pathway and Plk1. (2012)
  • Author(s): Kasahara, K.
  • Organizer: Global CEO the 4th International Symposium "Global COE Symposium on Nuro-Tumor Biology and Medicine"
  • Place of Presentation: Westin Nagoya Castle, Nagoya
  • Year and Date: 2012-11-15
• [Presentation] Novel mitotic signalling crosstalk between PI3K -Akt pathway and Plk1. (2012)
  • Author(s): Goto H, Kasahara K, Izawa I, Kiyono T, Watanabe N, Elowe S, Nigg EA, Inagaki M.
  • Organizer: The 52nd Annual Meeting of the American Society for Cell Biology.
  • Place of Presentation: Moscone Center, San Francisco
  • Year and Date: 2012-10-17
• [Presentation] Complex formation between Plk1 and 14-3-3 gamma is essential for metaphase-anaphase transition. (2012)
  • Author(s): Kousuke Kasahara、Masaki Inagaki
  • Organizer: Mini-symposium "STRESS SIGNALS & RESPONSES"
  • Place of Presentation: Abo University, Turku, Finland
  • Year and Date: 2012-09-27
• [Presentation] pathway controls mitotic progression through Plk1. (2012)
  • Author(s): 笠原広介、後藤英仁、井澤一郎、渡邉信元、清野透、稲垣昌樹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌ホテル・札幌
  • Year and Date: 2012-09-19
• [Presentation] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation. (2012)
  • Author(s): 後藤英仁、李萍、笠原広介、松山真、王中華・谷田部恭、清野透、稲垣昌樹
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会・ワークショップ
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2012-05-30
• [Presentation] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation. (2012)
  • Author(s): 後藤英仁、李萍、笠原広介、松山真、王中華・谷田部恭、清野透、稲垣昌樹
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2012-05-29
• [Presentation] PI3K-Akt pathway controls Polo -like kinase 1 (Plk1). (2012)
  • Author(s): 笠原広介、後藤英仁、井澤一郎、渡邉信元、清野透、稲垣昌樹
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会・ポスターフラッシュトーク
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2012-05-28
• [Presentation] PI3K-Akt pathway controls P olo-like kinase 1 (Plk1). (2012)
  • Author(s): 笠原広介、後藤英仁、井澤一郎、渡邉信元、清野透、稲垣昌樹
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会・ポスターフラッシュトーク
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2012-05-28
• [Presentation] Emerging role of the ubiqutiin-proteasome system in primary cilia assembly. (2012)
  • Author(s): 笠原広介
  • Organizer: GCOE第五回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ・大府
  • Year and Date: 2012-02-01
• [Presentation] The PI3K-Akt pathway controls mitotic progression through Plk1 (2012)
  • Author(s): 笠原広介
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌（札幌）
• [Presentation] Novel mitotic signaling pathway from Akt/PKB to Polo-like kinase 1 (Plk1) (2012)
  • Author(s): 笠原広介
  • Organizer: 第4回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ(愛知・大府)
• [Presentation] p90 RSK (Ribosomal S6 Kinase) によるChk1-Ser280のリン酸化修飾. (2011)
  • Author(s): 李萍、後藤英仁、松山真、笠原広介、村上善子、谷田部恭、清野透、稲垣昌樹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜・横浜
  • Year and Date: 2011-12-16
• [Presentation] 新規リン酸化反応による分裂キナーゼPlk1の活性制御メカニズム. (2011)
  • Author(s): 笠原広介、後藤英仁、井澤一郎、渡邉信元、清野透、稲垣昌樹
  • Organizer: 第34回日本分子生物学会年会・ワークショップ
  • Place of Presentation: パシフィコ横浜・横浜
  • Year and Date: 2011-12-15
• [Presentation] Chk1 phosphorylation by p90 Ribosomal S6 kinase (p90 RSK). (2011)
  • Author(s): Goto, H., Li, P., Kiyono, T., Matsuyama, M., Kasahara, K., Murakami, Y., Yatabe, Y. and Inagaki, M.
  • Organizer: The 51th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Colorado Convention Center, Denver
  • Year and Date: 2011-12-04
• [Presentation] 14-3-3 gamma activates Plk1 and controls mitotic progression. (2011)
  • Author(s): Kasahara, K., Goto, H., Izawa and Inagaki, M.
  • Organizer: The 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya Congress Center, Nagoya
  • Year and Date: 2011-10-03
• [Presentation] Chk1 phosphorylation at Ser280 by p90 ribosomal kinase (p90 RSK). (2011)
  • Author(s): Goto, H., Li, P., Yatabe, Y., Kasahara, K., Matsuyama, M., Kiyono, T. and Inagaki, M.
  • Organizer: The 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya Congress Center, Nagoya
  • Year and Date: 2011-10-03
• [Presentation] 14-3-3 gamma activates Plk1 and controls mitotic progression (2011)
  • Author(s): 笠原広介
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
• [Presentation] 増殖刺激応答におけるChk1-Ser280のリン酸化反応. (2010)
  • Author(s): 李萍、後藤英仁、笠原広介、松山誠、清野透、稲垣昌樹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2010-12-08
• [Presentation] 中心体ではなく核に局在するChk1が細胞周期において重要な役割を果たす. (2010)
  • Author(s): 松山誠、後藤英仁、笠原広介、川上和孝、井澤一郎、榎本将人、小堀恭子、中西真、清野透、五島直樹、稲垣昌樹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 国際会議場・神戸
  • Year and Date: 2010-12-08
• [Presentation] PP2A antagonizes Chk1 phosphorylation by ATR through 14-3-3 proteins in DNA damage checkpoint. (2010)
  • Author(s): 笠原広介、後藤英仁、清野透、稲垣昌樹
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・大阪
  • Year and Date: 2010-09-22
• [Presentation] 増殖刺激応答におけるChk1-Ser280のリン酸化反応. (2010)
  • Author(s): 李萍、後藤英仁、笠原広介、清野透、稲垣昌樹
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・大阪
  • Year and Date: 2010-09-22
• [Presentation] PP2A antagonizes Chk1 phosphorylation by ATR through 14-3-3 proteins in DNA damage checkpoint. (2010)
  • Author(s): 笠原広介
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] Complex formation between Plk1 and 14-3-3 gamma is essential for metapahse to anaphase transition
  • Author(s): 笠原広介
  • Organizer: Mini-Symposium on “Stress Signals & Responses”, Abo Akademi University Center of Excellence “Cell stress and Molecular Aging”
  • Place of Presentation: オーボ大学（フィンランド・トゥルク）
  • Invited
• [Presentation] Novel mitotic signalling crosstalk between PI3K-Akt pathway and Plk1
  • Author(s): 笠原広介
  • Organizer: Global COE the 4th International Symposium
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] PI3K-Akt pathway controls Polo-like kinase 1 (Plk1)
  • Author(s): 笠原広介
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場（神戸）
• [Book] 細胞周期フロンティア『抗リン酸化抗体と細胞周期研究』 (2010)
  • Author(s): 笠原広介
  • Publisher: 共立出版株式会社