Biomarker discovery in non-alcoholic steatohepatitis model mice
Project/Area Number |
22790104
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Biological pharmacy
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Research Institution | 独立行政法人医薬基盤研究所 |
Principal Investigator |
TSUNODA Shin-ichi 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (90357533)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 分子生物学 / プロテオミクス |
Research Abstract |
Non-alcoholic steatohepatitis(NASH) is recently recognized as a metabolic syndrome-related disease and proved to be an important risk factor for liver cirrhosis and cancer. Number of patient with NASH is rapidly increasing in economically advanced countries. Since molecular pathogenesis of NASH has not been cleared, precise diagnosis and effective therapy for NASH has not been established yet. To overcome such a situation, we searched disease-related proteins by using NASH model mice, CDAA-fed mice or TSOD mice. Proteome of liver tissues of NASH model mice and normal mice were compared by 2-dimentional differential gel electrophoresis(2D-DIGE). Proteins of differential expression were recovered from the gel and then identified by mass spectrometer. Further analysis of these proteins would lead to elucidation of molecular mechanism of development of NASH and development of promising diagnosis and therapeutic methods.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung2011
Author(s)
Yoshida Y, Yamashita T, Nagano K, Imai S, Nabeshi H, Yoshikawa T, Yoshioka Y, Abe Y, Kamada H, Tsutsumi Y, Tsunoda S
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Journal Title
Biochem Biophys Res Commun
Volume: 405
Issue: 4
Pages: 610-614
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of a novel antibody proteomics system using a phage antibody library for efficient screening of tumor-related biomarker proteins2011
Author(s)
Imai S, Nagano K, Yoshida Y, Okamura T, Yamashita T, Abe Y, Yoshikawa T, Yoshioka Y, Kamada H, Mukai Y, Nakagawa S, Tsutsumi Y, Tsunoda S
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Journal Title
Biomaterials
Volume: 32
Issue: 1
Pages: 162-169
DOI
Related Report
Peer Reviewed
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[Journal Article] Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung.2011
Author(s)
Yoshida Y., Yamashita T., Nagano K., Imai S., Nabeshi H., Yoshikawa T., Yoshioka Y., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
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Journal Title
Biochem.Biophys.Res.Comnun.
Volume: 405(4)
Pages: 610-614
Related Report
Peer Reviewed
-
[Journal Article] Development of a novel antibody proteomics system using a phage antibody library for efficient screening of tumor-related biomarker proteins.2011
Author(s)
Imai S., Nagano K., Yoshida Y., Okamura T., Yamashita T., Abe Y., Yoshikawa T., Yoshioka Y., Kamada H., Mukai Y., Nakagawa S., Tsutsumi Y., Tsunoda S.
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Journal Title
Biomaterials
Volume: 32(1)
Pages: 162-169
Related Report
Peer Reviewed
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[Presentation] Proteomics-based analysis of non-alcoholic fatty liver disease(NAFLD) in mice2010
Author(s)
Watanabe T., Yamashita T., Nagano K., Kanasaki S., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S
Organizer
HUPO2010 World Congres
Place of Presentation
Sydney
Year and Date
2010-09-20
Related Report
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[Presentation] Proteomics-based analysis of non-alcoholic fatty liver disease (NAFLD) in mice2010
Author(s)
Watanabe T., Yamashita T., Nagano K., Kanasaki S., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
Organizer
HUPO2010 World Congress
Place of Presentation
Sydney, Australia
Year and Date
2010-09-20
Related Report