Development of specific and highly sensitive method to detect nitroxyl by mass spectrometry

• Project/Area Number: 22790112
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: Kyoto University
• Principal Investigator: TSUMOTO Hiroki 京都大学, 大学院・薬学研究科, 特定助教 (00409385)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生物活性物質 / ニトロキシル / 質量分析

Research Abstract

This study aimed to develop specific and highly sensitive method to detect nitroxyl(HNO) by mass spectrometry(MS). A water-soluble thiol compound containing basic moiety to enhance MS signal intensity was synthesized. Sulfinamide produced by the reaction of thiol compound with HNO was detected by multiple reaction monitoring(MRM). Our results suggested that sulfinamide is a useful marker for detection of HNO.