Development of evaluation methods for comparability of mesenchymal stem cells based on glycans

• Project/Area Number: 22790125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: National Institute of Health Sciences
• Principal Investigator: HASHII Noritaka 国立医薬品食品衛生研究所, 生物薬品部, 第一室長 (20425672)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 医薬品化学 / 再生医学 / 再生医療 / 分析化学 / 糖鎖生物学

Research Abstract

For full-scale practical use of cell therapeutic products from MSCs, development of a method for distinguishing MSCs from differentiated cells is needed. We demonstrated the glycan profile alterations during early-stage of MSC differentiations. Glycan profiles can be used as a molecular index for discrimination of MSCs from differentiated cells.

Research Products

• [Journal Article] Glycomic analysis by liquid chromatography/mass spectrometry (2012)
  • Author(s): N. Hashii, S. Nakazawa, N. Kawasaki
  • Journal Title: YAKUGAKU ZASSHI Volume: 132 Pages: 489-497
  • Peer Reviewed
• [Journal Article] α1-3/4 fucosylation at Asn 241 ofβ-haptoglobin is a novel marker for colon cancer : A combinatorial approach for development of glycan biomarkers (2012)
  • Author(s): S. Y. Park, S. H. Lee, N. Kawasaki, S. Itoh, K. Kang, Ryu S. Hee, N. Hashii, J. M. Kim, J. Y. Kim, Kim J. Hoe, Kim
  • Journal Title: Int. J. Cancer Volume: 130 Pages: 2366-2376
  • Peer Reviewed
• [Journal Article] α1-3/4 fucosylation at Asn 241 of β-haptoglobin is a novel marker for colon cancer : A combinatorial approach for development of glycan biomarkers (2012)
  • Author(s): Park, S.Y., Lee, S.H., Kawasaki, N., Itoh, S., Kang, K., Hee, Ryu, S., Hashii, N., Kim, J.M., Kim, J.Y., Hoe, Kim, J.
  • Journal Title: Int.J.Cancer Volume: 130 Pages: 2366-2376
  • Peer Reviewed
• [Presentation] 糖鎖プロファイルによる間葉系幹細胞の分化初期における細胞の識別 (2011)
  • Author(s): 橋井則貴,黄笑宇,栗林亮佑,川崎ナナ
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-22
• [Presentation] 液体クロマトグラフィー/質量分析及び主成分分析による糖鎖プロファイルのデータマイニング法の評価 (2011)
  • Author(s): 橋井則貴,原園景,栗林亮佑,中澤志織,川崎ナナ
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府)
  • Year and Date: 2011-09-15
• [Presentation] Quantitative glycan analysis during neural differentiation of human mesenchymal stem cells by liquid chromatography/mass spectrometry (2010)
  • Author(s): N. Hashii, X. Huang, N. Kawasaki, T. Yamaguchi
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convention Center(Honolulu)
  • Year and Date: 2010-12-17
• [Presentation] Quantitative glycan analysis during neural differentiation of human mesenchymal stem cells by liquid chromatography/mass spectrometry. (2010)
  • Author(s): Hashii, Noritaka
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convention Center(Honolulu)
  • Year and Date: 2010-12-17
• [Presentation] Identification of cell therapeutic products from stem cells based on glycans (2010)
  • Author(s): N. Hashii, X. Huang, N. Kawasaki
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] Identification of cell therapeutic products from stem cells based on glycans. (2010)
  • Author(s): Hashii, Noritaka
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] Differential glycan analysis during neural differentiation of human mesenchymal stem cells by quantitative glycan profiling using liquid chromatography/mass spectrometry (2010)
  • Author(s): N. Hashii, X. Huang, N. Kawasaki, T. Yamaguchi
  • Organizer: The 25^<th> International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2010-08-05
• [Presentation] Differential glycan analysis during neural differentiation of human mesenchymal stem cells by quantitative glycan profiling using liquid chromatography/mass spectrometry. (2010)
  • Author(s): Hashii, Noritaka
  • Organizer: The 25^<th> International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2010-08-05