Analysis of role of transcription factor Nrf1 in protective response to heavy metals
| Project/Area Number |
22790128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 重金属 / カドミウム / メチル水銀 / Nrf1 / 血管内皮細胞 / 細胞応答システム / 抗酸化剤応答配列 / 生体防御システム |
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| Research Abstract |
Heavy metals such as cadmium and methylmercury are environmental pollutants that can target the vascular endothelium. In this study, we investigated the participation of transcription factor Nrf1 in cellular protective response to heavy metals. siRNA-mediated knockdown of Nrf1 resulted in an increase in sensitivity to cadmium and methylmercury in bovine aortic endothelial cells. Furthermore, exposure of cells to cadmium caused activation of Nrf1, leading to up-regulation of downstream genes such as NAD(P)H : quinone oxidereductase 1 and peroxiredoxin 1. Taken together, these results suggests that Nrf1 acts as a defense system against heavy metals. induced toxicity.
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Report
(3 results)
Research Products
(49 results)
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[Journal Article] Initial response and cellular protection through the Keap1/Nrf2 system during exposure of primary mouse hepatocytes to 1,2-naphthoquinone2011
Author(s)
Miura T, Shinkai Y, Jiang HY, IwamotoN, Sumi D, Taguchi K, Yamamoto M, Jinno H, Tanaka-Kagawa T, Cho AK, Kumagai Y
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Journal Title
Chemical Research in Toxicology
Volume: 24
Pages: 559-567
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