Diagnostic method of Helicobacter heilmannii infection based on genetic analysis

• Project/Area Number: 22790136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: MIKI Ikuya 神戸薬科大学, 薬学部, 講師 (30420467)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ヘリコバクターハイルマニ / ウレアーゼ

Research Abstract

Helicobacter heilmannii infection is possibly associated with gastric MALT lymphoma in mice model and human, and however, its diagnostic method has not been established yet. In this study, we succeed in the diagnosis of its infection with gastric mucosa by analyzing pathogenic genes of H. pylori and H. heilmannii. In the next step, we tried to establish the serodiagnosis using monoclonal antibody, but our antibody showed cross-reactivity. Therefore, we are going on analyzing the other virulent genes.

Research Products

• [Journal Article] Neonatal Fc receptor for IgG(FcRn) expressed in the gastric epithelium regulates bacterial infection in mice (2012)
  • Author(s): Ben Suleiman Y, Yoshida M, Nishiumi S, Tanaka H, Mimura T, Nobutani K, Yamamoto K, Takenaka M, Aoganghua A, Miki I, Ota H, Takahashi S, Matsui H, Nakamura M, Blumberg RS, Azuma T
  • Journal Title: Mucosal Immunol Volume: 5 Pages: 87-98
• [Journal Article] IFN-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection (2011)
  • Author(s): Mimura T, Yoshida M, Nishiumi S, Tanaka H, Nobutani K, Takenaka M, Suleiman YB, Yamamoto K, Ota H, Takahashi S, Matsui H, Nakamura M, Miki I, Azuma T
  • Journal Title: FEMS Immunol Med Microbiol Volume: 63 Pages: 25-34
• [Journal Article] Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells (2011)
  • Author(s): Yamamoto K, Tanaka H, Nishitani Y, Nishiumi S, Miki I, Takenaka M, Nobutani K, Mimura T, Ben Suleiman Y, Mizuno S, Kawai M, Uchiyama I, Yoshida M, Azuma T
  • Journal Title: Microbes Infect Volume: 13 Pages: 697-708