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Investigation of manganese transport system in neuronal cells

Research Project

Project/Area Number 22790138
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Environmental pharmacy
Research InstitutionTokushima Bunri University

Principal Investigator

FUJISHIRO Hitomi  徳島文理大学, 薬学部, 助教 (10389182)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords中毒学 / マンガン / 輸送 / パーキンソニズム / 亜鉛 / 神経 / ZIP8 / ZIP14 / 金属 / 耐性
Research Abstract

Manganese(Mn) is an essential element, but exposure to excess amount of Mn causes symptoms similar to Parkinson's disease. Although several transporters may be involved in Mn uptake, only the role of DMT1 and transferrin receptor have been examined regarding Mn transport in nervous systems. To confirm the contribution of DMT1, we first examined the effects of DMT1 siRNA. As a result, the uptake of Mn in human neuroblastoma SH-SY5Y cells was suppressed by introduction of siRNA of DMT1.Next, we focused on zinc transporters, ZIP8 and ZIP14, which can transport Mn as well as Zn and Cd. The uptakes of Mn^<2+> and Cd^<2+> were suppressed by the introduction of siRNA of ZIP14, but not by ZIP8, in both SH-SY5Y and mouse hippocampus HT22 cells. These data suggest that ZIP14 in addition to DMT1 might play an important role in Mn transport in neuronal cells.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (41 results)

All 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (30 results)

  • [Journal Article] Roles of ZIP8, ZIP14, and DMT1 in transport of cadmium and manganese in mouse kidney proximal tubule cells2012

    • Author(s)
      Fujishiro, H., Yano, Yu., Takada, Y., Tanihara, M., Himeno, S.
    • Journal Title

      Metallomics

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Multiple Metal Transporters in Cadmium Transport2011

    • Author(s)
      Fujishiro, H., Himeno, S.
    • Journal Title

      Biomed. Res. Trace Elements

      Volume: 22(1) Pages: 1-6

    • NAID

      10028117932

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cross-resistance of cadmium-resistant cells to manganese is associated with reduced accumulation of both cadmium and manganese2011

    • Author(s)
      Fujishiro, H., Kubota, K., Inoue, D., Inoue, A., Yanagiya, T., Enomoto, S., Himeno, S.
    • Journal Title

      Toxicology

      Volume: 280(3) Pages: 118-125

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells2011

    • Author(s)
      Shimizu, Y., Fujishiro, H., Matsumoto, K., Sumi, D., Satoh, M. and Himeno, S.
    • Journal Title

      J. Toxicol. Sci

      Volume: 36(1) Pages: 135-139

    • NAID

      10027420605

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] High sensitivity of RBL-2H3 cells to cadmium and manganese : an implication of the role of ZIP82011

    • Author(s)
      Fujishiro, H., Doi, M., Enomoto, S., Himeno, S.
    • Journal Title

      Metallomics

      Volume: 3(7) Pages: 710-718

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Multiple Metal Transporters in Cadmium Transport2011

    • Author(s)
      Fujishiro, H., Himeno, S.
    • Journal Title

      Biomed.Res.Trace Elements.

      Volume: 22(1) Pages: 1-6

    • NAID

      10028117932

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-resistance of cadmium-resistant cells to manganese is associated with reduced accumulation of both cadmium and manganese.2011

    • Author(s)
      Fujishiro, H., Kubota, K., Inoue, D., Inoue, A., Yanagiya, T., Enomoto, S., Himeno, S.
    • Journal Title

      Toxicology

      Volume: 280(3) Pages: 118-125

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells.2011

    • Author(s)
      Shimizu Y., Fujishiro, H., Matsumoto, K., Sumi, D., Satoh, M., Himeno, S.
    • Journal Title

      J.Toxicol.Sci.

      Volume: 36(1) Pages: 135-139

    • NAID

      10027420605

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA microarray analysis of the liver of mice treated with cobalt chloride2010

    • Author(s)
      Matsumoto, K., Fujishiro, H., Satoh, M., Himeno, S.
    • Journal Title

      J. Toxicol. Sci

      Volume: 35(6) Pages: 935-939

    • NAID

      10026963300

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA microarray analysis of the liver of mice treated with cobalt chloride.2010

    • Author(s)
      Matsumoto, K., Fujishiro, H., Satoh M., Himeno, S.
    • Journal Title

      J.Toxicol.Sci.

      Volume: 36(6) Pages: 935-939

    • NAID

      10026963300

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Multiple Metal Transporters in Cadmium Transport.

    • Author(s)
      Fujishiro, H., Himeno, S.
    • Journal Title

      Biomed.Res.Trace Elements.

      Volume: (in press)

    • NAID

      10028117932

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 腎臓近位尿細管細胞におけるカドミウム輸送および金属輸送体の発現2012

    • Author(s)
      矢野悠, 高田侑那, 藤代瞳, 姫野誠一郎
    • Organizer
      日本薬学会132年会
    • Place of Presentation
      北海道
    • Year and Date
      2012-03-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 神経芽細胞腫SH-SY5Y細胞における亜鉛輸送体ZIP14の役割2012

    • Author(s)
      吉田真梨、中野佑香、藤代瞳、姫野誠一郎
    • Organizer
      日本薬学会132年会
    • Place of Presentation
      北海道
    • Year and Date
      2012-03-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 腎臓近位尿細管細胞におけるカドミウム輸送および金属輸送体の発現2012

    • Author(s)
      矢野悠, 高田侑那, 藤代瞳, 姫野誠一郎
    • Organizer
      日本薬学会132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] 神経芽細胞腫SH-SY5Y細胞における亜鉛輸送体ZIP14の役割2012

    • Author(s)
      吉田真梨、中野佑香、藤代瞳、姫野誠一郎
    • Organizer
      日本薬学会132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] マンガンおよびカドミウム耐性RBL-2H3細胞の性状解析2011

    • Author(s)
      藤代瞳、大橋俊直、詫間美紀、姫野誠一郎
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2011
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-08
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 神経細胞におけるマンガン取り込みに関与する輸送体の探索2011

    • Author(s)
      藤代瞳、中野佑香、吉田真梨、角大悟、姫野誠一郎
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2011
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-08
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 腎臓近位尿細管細胞へのカドミウム輸送におけるZIP8, ZIP14, DMT1の役割2011

    • Author(s)
      藤代瞳、矢野悠、高田侑那、姫野誠一郎
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2011
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 腎臓近位尿細管細胞へのカドミウム輸送におけるZIP8,ZIP14,DMT1の役割2011

    • Author(s)
      藤代瞳、矢野悠、高田侑那、姫野誠一郎
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2011
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腎臓近位尿細管細胞へのカドミウム輸送における亜鉛輸送体の役割2011

    • Author(s)
      藤代瞳, 矢野悠, 姫野誠一郎
    • Organizer
      フォーラム2011:衛生薬学・環境トキシコロジー
    • Place of Presentation
      金沢
    • Year and Date
      2011-10-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 神経細胞へのマンガン輸送における亜鉛輸送体の役割2011

    • Author(s)
      藤代瞳, 中野佑香, 姫野誠一郎
    • Organizer
      フォーラム2011:衛生薬学・環境トキシコロジー
    • Place of Presentation
      金沢
    • Year and Date
      2011-10-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] RBL-2H3細胞由来カドミウム耐性細胞、マンガン耐性細胞の性状解析2011

    • Author(s)
      藤代瞳、大橋俊直、角大悟、姫野誠一郎
    • Organizer
      第38回日本トキシコロジー学会学術年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-07-11
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] RBL-2H3細胞由来マンガン耐性細胞の性状解析2011

    • Author(s)
      大橋俊直、藤代瞳、姫野誠一郎
    • Organizer
      第22回微量元素学会
    • Place of Presentation
      京都
    • Year and Date
      2011-07-01
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 神経芽細胞腫SH-SY5Y細胞におけるマンガン輸送機構の検討2011

    • Author(s)
      中野佑香、藤代瞳、姫野誠一郎
    • Organizer
      第22回微量元素学会
    • Place of Presentation
      京都
    • Year and Date
      2011-07-01
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Cadmium transport in proximal tubule cells derived from mouse kidney2011

    • Author(s)
      Yu YANO, Hitomi FUJISHIRO, and Seiichiro HIMEN
    • Organizer
      第21回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      千葉
    • Year and Date
      2011-05-30
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Screening study of low-molecular-weight compounds for the modulation of cellular cadmium transport2011

    • Author(s)
      Hitomi FUJISHIRO, Yukari ATSUMI, Atsuhito INOUE, and Seiichiro HIMENO
    • Organizer
      第21回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      千葉
    • Year and Date
      2011-05-30
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] カップ培養系を用いた腎臓近位尿細管細胞におけるカドミウム輸送機構の検討2011

    • Author(s)
      矢野悠、藤代瞳、姫野誠一郎
    • Organizer
      日本薬学会131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Interferon-betaの産生抑制を介した亜ヒ酸によるNO産生阻害機構2011

    • Author(s)
      津山博匡、角大悟、藤代瞳、姫野誠一郎
    • Organizer
      第16回ヒ素シンポジウム
    • Place of Presentation
      旭川
    • Year and Date
      2011-02-05
    • Related Report
      2011 Final Research Report
  • [Presentation] カドミウムの排泄に関与する輸送体の探索2010

    • Author(s)
      井上敦仁、藤代瞳、姫野誠一郎
    • Organizer
      第2回メタロミクス研究フォーラム
    • Place of Presentation
      京都
    • Year and Date
      2010-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] カドミウムの排泄に関与する輸送体の探索2010

    • Author(s)
      井上敦仁、藤代瞳、姫野誠一郎
    • Organizer
      第二回メタロミクス研究フォーラム
    • Place of Presentation
      京都
    • Year and Date
      2010-11-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] The role of ZIP8 in bicarbonate-dependent transport of cadmium and manganese in RBL-2H3 cells2010

    • Author(s)
      Hitomi FUJISHIRO and Seiichiro HIMENO
    • Organizer
      第60回藤原セミナー
    • Place of Presentation
      大阪
    • Year and Date
      2010-10-29
    • Related Report
      2011 Final Research Report
  • [Presentation] The role of ZIP8 in bicarbonate-dependent transport of cadmium and manganese in RBL-2H3 cells.2010

    • Author(s)
      Hitomi FUJISHIRO, Seiichiro HIMENO.
    • Organizer
      第60回藤原セミナー
    • Place of Presentation
      大阪
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 腎臓近位尿細管由来の不死化細胞(PT3)におけるカドミウム輸送2010

    • Author(s)
      藤代瞳, 矢野悠, 三好亜依, 姫野誠一郎
    • Organizer
      フォーラム2010:衛生薬学・環境トキシコロジー
    • Place of Presentation
      東京
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] RBL-2H3細胞におけるカドミウム、マンガン輸送機構2010

    • Author(s)
      藤代瞳、土肥美和子、姫野誠一郎
    • Organizer
      第5回トランスポーター研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-10
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Interaction of cadmium and manganese in cellular uptake and toxicity2010

    • Author(s)
      姫野誠一郎, 土肥美和子, 藤代瞳
    • Organizer
      VII International Congress of Toxicology
    • Place of Presentation
      バルセロナ
    • Year and Date
      2010-07-10
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] RBL-2H3細胞におけるカドミウム、マンガン感受性と輸送機構2010

    • Author(s)
      藤代瞳, 土肥美和子, 姫野誠一郎
    • Organizer
      第21回微量元素学会ミニシンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2010-07-04
    • Related Report
      2011 Final Research Report
  • [Presentation] RBL-2H3細胞におけるカドミウム、マンガン感受性と輸送機構2010

    • Author(s)
      藤代瞳, 土肥美和子, 姫野誠一郎
    • Organizer
      第21回微量元素学会
    • Place of Presentation
      京都
    • Year and Date
      2010-07-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Establishment and characterization of manganese-and cadmium-resistant RBL-2H3 cells2010

    • Author(s)
      Hitomi FUJISHIRO, Miwako Doi, Seiichiro HIMENO
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-06-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of bicarbonate on cellular uptake of cadmium and manganese2010

    • Author(s)
      Hitomi FUJISHIRO, Miwako Doi, Ai Miyoshi, Seiichiro HIMENO
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-06-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Establishment and characterization of manganese- and cadmium-resistant RBL-2H3 cells.2010

    • Author(s)
      Hitomi FUJISHIRO, Miwako Doi, Seiichiro HIMENO.
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of bicarbonate on cellular uptake of cadmium and manganese.2010

    • Author(s)
      Hitomi FUJISHIRO, Miwako Doi, Ai Miyoshi, Seiichiro HIMENO.
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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