Investigation of manganese transport system in neuronal cells
| Project/Area Number |
22790138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 中毒学 / マンガン / 輸送 / パーキンソニズム / 亜鉛 / 神経 / ZIP8 / ZIP14 / 金属 / 耐性 |
|---|
| Research Abstract |
Manganese(Mn) is an essential element, but exposure to excess amount of Mn causes symptoms similar to Parkinson's disease. Although several transporters may be involved in Mn uptake, only the role of DMT1 and transferrin receptor have been examined regarding Mn transport in nervous systems. To confirm the contribution of DMT1, we first examined the effects of DMT1 siRNA. As a result, the uptake of Mn in human neuroblastoma SH-SY5Y cells was suppressed by introduction of siRNA of DMT1.Next, we focused on zinc transporters, ZIP8 and ZIP14, which can transport Mn as well as Zn and Cd. The uptakes of Mn^<2+> and Cd^<2+> were suppressed by the introduction of siRNA of ZIP14, but not by ZIP8, in both SH-SY5Y and mouse hippocampus HT22 cells. These data suggest that ZIP14 in addition to DMT1 might play an important role in Mn transport in neuronal cells.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] Roles of ZIP8, ZIP14, and DMT1 in transport of cadmium and manganese in mouse kidney proximal tubule cells2012
Author(s)
Fujishiro, H., Yano, Yu., Takada, Y., Tanihara, M., Himeno, S.
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Journal Title
Metallomics
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Cross-resistance of cadmium-resistant cells to manganese is associated with reduced accumulation of both cadmium and manganese2011
Author(s)
Fujishiro, H., Kubota, K., Inoue, D., Inoue, A., Yanagiya, T., Enomoto, S., Himeno, S.
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Journal Title
Toxicology
Volume: 280(3)
Pages: 118-125
Related Report
Peer Reviewed
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[Journal Article] Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells2011
Author(s)
Shimizu, Y., Fujishiro, H., Matsumoto, K., Sumi, D., Satoh, M. and Himeno, S.
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Journal Title
J. Toxicol. Sci
Volume: 36(1)
Pages: 135-139
NAID
Related Report
Peer Reviewed
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[Journal Article] Cross-resistance of cadmium-resistant cells to manganese is associated with reduced accumulation of both cadmium and manganese.2011
Author(s)
Fujishiro, H., Kubota, K., Inoue, D., Inoue, A., Yanagiya, T., Enomoto, S., Himeno, S.
-
Journal Title
Toxicology
Volume: 280(3)
Pages: 118-125
Related Report
Peer Reviewed
-
[Journal Article] Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells.2011
Author(s)
Shimizu Y., Fujishiro, H., Matsumoto, K., Sumi, D., Satoh, M., Himeno, S.
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Journal Title
J.Toxicol.Sci.
Volume: 36(1)
Pages: 135-139
NAID
Related Report
Peer Reviewed
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