Construction of Evaluation Model for Adipocyte Hypertrophy in vitro and Study on Elucidation of Mechanism of Adipocyte Enlargement

• Project/Area Number: 22790139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Tokushima Bunri University
• Principal Investigator: KADOTA Yoshito 徳島文理大学, 薬学部, 助教 (60461365)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞・組織 / 脂質 / 肥満

Research Abstract

We constructed 3D cell culture system of evaluation model for adipocyte hypertrophy in vitro. As a result, enlarged adipocytes with a unilocular lipid droplet were observed. We also analyzed expression patterns of adipocyte enlargement maker gene, MEST. MEST mRNA was upregulated by increasing intracellular cAMP during adipocyte differentiation. We also found an enhancer region sensitive to the cAMP elevation, which is located 5'-upstream of the MEST promoter.

Research Products

• [Journal Article] Gene expression of mesoderm-specific transcript is upregulated as preadipocytes differentiate to adipocytes in vitro (2012)
  • Author(s): Kadota Y, Yanagawa M, Nakaya T, Kawakami T, Sato M, Suzuki S.
  • Journal Title: J Physiol Sci Volume: (in press)
  • NAID: 10031059780
  • Peer Reviewed
• [Journal Article] Differential effects of cobalt and mercury on lipid metabolism in the white adipose tissue of high-fat diet-induced obesity mice (2012)
  • Author(s): Kawakami T, Hanao N, Nishiyama K, Kadota Y, Inoue M, Sato M, Suzuki S.
  • Journal Title: Toxicol Appl Pharmacol Volume: 258 Pages: 32-42
  • Peer Reviewed
• [Journal Article] Differential effects of cobalt and mercury on lipid metabolism in the white adipose tissue of high-fat diet-induced obesity mice (2012)
  • Author(s): Kawakami T, Hanao N, Nishiyama K, Kadota Y, Inoue M, Sato M, Suzuki S
  • Journal Title: Toxicol Appl Pharmacol Volume: 258(1) Issue: 1 Pages: 32-42
  • DOI: 10.1016/j.taap.2011.10.004
  • Peer Reviewed
• [Journal Article] Early changes induced by environmental stresses in insulin sensitivity-related genes (2011)
  • Author(s): Sato M, Ishibashi S, Higashimoto M, Kadota Y, Kawakami T, Suzuki S.
  • Journal Title: Eur J Pharmacol Volume: 668 Pages: 472-6
  • Peer Reviewed
• [Journal Article] Early changes induced by environmental stresses in insulin sensitivity-related genes (2011)
  • Author(s): M.Sato, S.Ishibashi, M.Higashimoto, Y.Kadota, T.Kawakami, S.Suzuki
  • Journal Title: European Journal of Pharmacology Volume: 668 Issue: 3 Pages: 472-476
  • DOI: 10.1016/j.ejphar.2011.07.034
  • Peer Reviewed
• [Journal Article] Development of high-fat-diet-induced obesity in female metallothionein-null mice (2010)
  • Author(s): Sato M, Kawakami T, Kondoh M, Takiguchi M, Kadota Y, Himeno S, Suzuki S.
  • Journal Title: FASEB J Volume: 24 Pages: 2375-84
  • Peer Reviewed
• [Journal Article] Development of high-fat-diet-induced obesity in female metallothionein-null mice. (2010)
  • Author(s): 佐藤政男
  • Journal Title: The FASEB Journal Volume: 24 Pages: 2375-2384
  • Peer Reviewed
• [Presentation] 脂肪細胞関連遺伝子Mestは脂肪細胞分化時に発現が増大する (2012)
  • Author(s): 門田佳人、柳川真澄、中矢誠子、細野佑衣、川上隆茂、佐藤政男、鈴木真也
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌・北海道大学
  • Year and Date: 2012-03-31
• [Presentation] 脂肪細胞肥大化促進遺伝子Mestのマウス培養脂肪細胞分化誘導時における発現変動に関する研究 (2011)
  • Author(s): 門田佳人、柳川真澄、中矢誠子、川上隆茂、佐藤政男、鈴木真也
  • Organizer: フォーラム2011衛生薬学・環境トキシコロジー
  • Place of Presentation: 金沢・エクセルホテル東急
  • Year and Date: 2011-10-28
• [Presentation] 脂肪細胞肥大化促進遺伝子MESTの脂肪細胞分化時における発現機構の解析 (2011)
  • Author(s): 門田佳人、柳川真澄、中矢誠子、川上隆茂、佐藤政男、鈴木真也
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡・ツインメッセ静岡
  • Year and Date: 2011-03-30
• [Remarks]
  • URL: http://ph.bunri-u.ac.jp/lab11/
• [Remarks]
  • URL: http://p.bunri-u.ac.jp/lab11/
• [Remarks]
  • URL: http://p.bunri-u.ac.jp/lab11/