Attempts to identify functional receptor for caliciviruses

• Project/Area Number: 22790142
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: OKA Tomoichiro 国立感染症研究所, ウイルス第二部, 主任研究官 (50356242)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 微生物 / 感染症学 / ウイルス / 感染症

Research Abstract

To identify calicivirus functional receptors, cellular factors binding to human Norovirus or Sapovirus-like particles have been screened using expression screening of human small intestine cDNA library. A novel plasmid-based reverse genetics system for feline calicivirus has been established. This system could be used for the production of infectious human calicivirus.

Research Products

• [Journal Article] Bioluminescence technologies to detect calicivirus protease activity in cell-free system and in infected cells (2011)
  • Author(s): Oka T, Takagi H, Tohya Y, Murakami K, Takeda N, Wakita T, Katayama K.
  • Journal Title: Antiviral Research Volume: 90(1) Pages: 9-16
  • URL: http://dx.doi.org/10.1016/j.antiviral.2011.02.002
• [Journal Article] Comparative site-directed mutagenesis in the catalytic amino acid triad in calicivirus protease (2011)
  • Author(s): Oka T, Murakami K, Wakita T, Katayama K.
  • Journal Title: Microbiology and Immunology Volume: 55(2) Issue: 2 Pages: 108-14
  • DOI: 10.1111/j.1348-0421.2010.00295.x
  • NAID: 10031121448
• [Journal Article] Bioluminescence technologies to detect calicivirus protease activity in cell-free system and in infected cells (2011)
  • Author(s): Oka T, Takagi H, Tohya Y, Murakami K, Takeda N, Wakita T、Katayama K
  • Journal Title: Antiviral Research Volume: 90 Pages: 9-16
  • Peer Reviewed
• [Journal Article] Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine noroviruses (2010)
  • Author(s): Kitajima M, Oka T, Takagi H, Tohya Y, Katayama H, Takeda N, Katayama K.
  • Journal Title: Journal of Virological Methods Volume: 169(2) Pages: 269-273
  • URL: http://dx.doi.org/10.1016/j.jviromet.2010.07.018
• [Journal Article] Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine noroviruses. (2010)
  • Author(s): Kitajima M, Oka T, Takagi H, Tohya Y, Katayama H, Takeda N, Katayama K.
  • Journal Title: Journal of Virological Methods. Volume: 169(2) Pages: 269-273
  • Peer Reviewed
• [Presentation] Surveillance of pathogens in outpatients with gastroenterlitis and genetic analysis of sapovirus strains between 2002 and 2009 in Kumamoto Prefecture (2011)
  • Author(s): Harada S, Nishimura K, Kiyota N, Matsumoto K, Yahiro S, Okada M, Katayama K, Oka T.
  • Organizer: Japan 16th Federation of Asian Veterinary Associations Congress 2011
  • Place of Presentation: フィリピン
• [Presentation] ノロウイルスのヒト腸管由来培養細胞への結合様式 (2010)
  • Author(s): 村上耕介、岡智一郎、脇田隆字、松田幹、片山和彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] カリシウイルスプロテアーゼcatalytic triad形成残基のポリプロテイン切断活性への重要性 (2010)
  • Author(s): 岡智一郎、横山勝、高木弘隆、本村和嗣、村上耕介、佐藤裕徳、脇田隆字、片山和彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] マウスノロウイルス(MNV)のマウス由来培養細胞での増殖性についての検討 (2010)
  • Author(s): 高木弘隆、北島正章、遠矢幸伸、岡智一郎、片山浩之、片山和彦、杉山和良
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] サポウイルスに対する単クローン抗体の解析 (2010)
  • Author(s): 北元憲利、岡智一郎、片山和彦、Hansman GS、三好龍也、田中智之
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] ノロウイルスのヒト腸管由来細胞への結合様式の解析 (2010)
  • Author(s): 村上耕介、岡智一郎、脇田隆字、松田幹、片山和彦
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] ネコカリシウイルスの新規リバースジェネティクス系の構築 (2010)
  • Author(s): 岡智一郎、高木弘隆、遠矢幸伸、村上耕介、脇田隆字、片山和彦
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] カリシウイルスの新知見 (2010)
  • Author(s): 岡智一郎
  • Organizer: ウイルス性下痢症研究会第22回学術集会
  • Place of Presentation: 徳島
• [Presentation] Antiviral Development (2010)
  • Author(s): Oka T, Yokoyama M, Takagi H, Tohya Y, Motomura K, Murakami K, Wakita T, Sato H, Katayama K.
  • Organizer: Fourth International Conference on Caliciviruses
  • Place of Presentation: チリ
• [Presentation] Antiviral Development (2010)
  • Author(s): Oka T., et al.
  • Organizer: Fourth International Conference on Caliciviruses
  • Place of Presentation: チリ(サンタクルス・プラザホテル)
• [Presentation] マウスノロウイルス(MNV)のマウス由来培養細胞での増殖性についての検討 (2010)
  • Author(s): 高木弘隆、北島正章、遠矢幸伸、岡豊一郎、片山浩之、片山和彦、杉山和良
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県(あわぎんホール)
• [Remarks]
  • URL: http://dx.doi.org/10.1016/j.antiviral.2011.02.002