Characterization of "Ribavirin transportsome" in the small intestine

• Project/Area Number: 22790145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Chiba University
• Principal Investigator: FURIHATA Tomomi 千葉大学, 大学院・薬学研究院, 助教 (80401008)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 薬物代謝 / 吸収 / リバビリン / C型肝炎 / プリンヌクレオシドホスホリラーゼ / 小腸 / 代謝 / purine nucleoside phosphorylase / 遺伝子多型

Research Abstract

The purpose of the present study was to identify a role of purine nucleoside phosphorylase (PNP) in ribavirin absorption. We showed that PNP expressed in human small intestine exclusively metabolized ribavirin to inactive nucleobase. We also showed that the level of PNP activity/expression was one of the determinant factors for the level of ribavirin absorption. Therefore, it can be concluded that PNP plays an important role in determining ribavirin plasma concentration through its metabolizing ability in human small intestine.

Research Products

• [Presentation] C型肝炎治療薬リバビリン薬効発現における核酸トランスポーターENT1の重要性 (2011)
  • Author(s): 降幡知巳, 福地由希菜, 飯倉南, 橋詰美里, 水口美紗, 長井美樹, 千葉寛
  • Organizer: 日光シンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2011-12-18
• [Presentation] C型肝炎治療薬リバビリン薬効発現における核酸トランスポーターENT1の重要性 (2011)
  • Author(s): 降幡知巳、福地由希菜、飯倉南、橋詰美里、水口美紗、長井美樹、千葉寛
  • Organizer: 日光シンポジウム
  • Place of Presentation: 日光(招待講演)
  • Year and Date: 2011-12-18
• [Presentation] Ribavirin metabolism by purine nucleoside phosphorylase : potential implications for ribavirin first-pass metabolism in the human small intestine (2011)
  • Author(s): Satoshi Kishida, Tomomi Furihata, Hanae Sugiura, Atsuko Kamiichi, Kan Chiba
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島
  • Year and Date: 2011-11-18
• [Presentation] Ribavirin metabolism by purine nucleoside phosphorylase : potential implications for ribavirin first-pass metabolism in the human small intestine (2011)
  • Author(s): Satoshi Kishida, Tomomi Furihata, Hanae Sugiura, Atsuko Kamiichi, Kan Chiba
  • Organizer: 4th Asia-pacific regional ISSX meeting
  • Place of Presentation: Tainan,Taiwan
  • Year and Date: 2011-04-23
• [Presentation] Ribavirin metabolism by purine nucleoside phosphorylase : potential implications for ribavirin first-pass metabolism in the human small intestine (2011)
  • Author(s): Satoshi Kishida, Tomomi Furihata, Hanae Sugiura, Atsuko Kamiichi, Kan Chiba
  • Organizer: 4th Asia-pacific regional ISSX meeting
  • Place of Presentation: 台南(台湾)
  • Year and Date: 2011-04-23
• [Presentation] RIBAVIRIN UPTAKE SYSTEMS ARE REQUIGED FOR THE ANITI-HEPATITIS C VIRUS ACTIVITY OF RIBAVIRIN (2010)
  • Author(s): Minami Iikura, Tomomi Furihata, Masanori Ikeda, Nobuyuki Kato, Kan Chiba
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Ribavirin uptake systems are required for the anti.hepatitis C virus activity of ribavirin (2010)
  • Author(s): Minami Iikura, Tomomi Furihata, Masanori Ikeda, Nobuyuki Kato, Kan Chiba.
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] C型肝炎治療薬リバビリン薬効発現における細胞内リバビリン取込み機構の重要性 (2010)
  • Author(s): 飯倉南, 降幡知巳, 池田正徳, 加藤宣之, 千葉寛
  • Organizer: 日本薬物動態学会第25回年会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-10-09
• [Presentation] RIBAVIRIN UPTAKE SYSTEMS ARE REQUIGED FOR THE ANITI-HEPATITIS C VIRUS ACTIVITY OF RIBAVIRIN (2010)
  • Author(s): Minami Iikura, Tomomi Furihata, Masanori Ikeda, Nobuyuki Kato, Kan Chiba
  • Organizer: 9th International ISSX Meeting
  • Place of Presentation: イスタンブール(トルコ)
  • Year and Date: 2010-09-06
• [Presentation] Ribavirin uptake systems are required for the anti.hepatitis C virus activity of ribavirin (2010)
  • Author(s): Minami Iikura, Tomomi Furihata, Masanori Ikeda, Nobuyuki Kato, Kan Chiba
  • Organizer: 9th International ISSX Meeting
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2010-09-05
• [Presentation] C型肝炎治療薬リバビリン薬効発現における細胞内リバビリン取込み機構の重要性 (2010)
  • Author(s): 飯倉南, 降幡知巳, 池田正徳, 加藤宣之, 千葉寛
  • Organizer: 第5回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-10
• [Presentation] マウスおよびラット肝における核酸トランスポーターCNT2の著しい種差 (2010)
  • Author(s): 岸田聡, 降幡知巳, 飯倉南, 福地由希菜, 橋詰美里, 長井美樹, 宮嶋篤志, 千葉寛
  • Organizer: 第5回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-10
• [Remarks]
  • URL: http://www.p.chiba-u.ac.jp/lab/yakubutu/framepage4.html
• [Remarks]
  • URL: http://www.p.chiba-u.ac.jp/lab/yakubutu/index.html
• [Remarks]
  • URL: http://www.p.chiba-u.ac.jp/lab/yakubutu/index.html