Pathophysiological role of riboflavin transporter RFT based on the clinical data

• Project/Area Number: 22790154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: YONEZAWA Atsushi 京都大学, 医学研究科, 助教 (90452341)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 薬学 / 薬物動態学 / ビタミンB2 / 先天性疾患 / 膜タンパク質 / 薬物生態学

Research Abstract

Riboflavin transporters had been identified. The purpose of this study is to clarify the physiological role of RFT1 and its homologues. RFT3, which is expressed in the brain, was identified. The functional characteristics of RFTs were elucidated. Heterozygous deletion in the RFT1 gene was found. In addition, RFT genes were registered in the HGNC database. In conclusion, we obtained novel findings in the physiological role of RFTs.

Research Products

• [Journal Article] Importance of the Multidrug and Toxin Extrus ion MATE/SLC47A Family to Pharmacokinetics, Pharmacodynamics/Toxicodynamics and Pharmacogenomics (2011)
  • Author(s): Yonezawa A, Inui K.
  • Journal Title: Br J Pharmacol Volume: 164(7) Issue: 7 Pages: 1817-1825
  • DOI: 10.1111/j.1476-5381.2011.01394.x
  • Peer Reviewed
• [Journal Article] Maternal riboflavin deficiency, resulting in transient neonatal-onset glutaric aciduria Type 2, is caused by a microdeletion in the riboflavin transporter gene GPR172B (2011)
  • Author(s): Ho G, Yonezawa A, Masuda S, Inui K, Sim KG, Carpenter K, Olsen RK, Mitchell JJ, Rhead WJ, Peters G, Christodoulou J.
  • Journal Title: Hum Mutat. Volume: 32(1) Issue: 1 Pages: 1976-1984
  • DOI: 10.1002/humu.21399
  • Peer Reviewed
• [Journal Article] Maternal riboflavin deficiency, resulting in transient neonatal-onset glutaric aciduria type 2, is caused by a microdeletion in the riboflavin transporter gene GPR172B. (2011)
  • Author(s): Ho, G., et al.
  • Journal Title: Human Mutation Volume: 32
  • Peer Reviewed
• [Journal Article] Identification and comparative functional characterization of a new human riboflavin transporter hRFT3 expressed in the brain (2010)
  • Author(s): Yao Y, Yonezawa A, Yoshimatsu H, Masuda S, Katsura T, Inui K.
  • Journal Title: J Nutr Volume: 40(7) Issue: 7 Pages: 1220-1226
  • DOI: 10.3945/jn.110.122911
  • Peer Reviewed
• [Journal Article] Identification and comparative functional characterization of a new human riboflavin transporter hRFT3 expressed in the brain. (2010)
  • Author(s): Yao, Y., et al.
  • Journal Title: Journal of Nutrition Volume: 40 Pages: 1220-1226
  • Peer Reviewed
• [Presentation] 第7回若手が拓く新しい薬剤学H+/有機カチオンアンチポータMATE1の機能低下による副作用発現の増強 (2012)
  • Author(s): 米澤淳、増田智先、桂敏也
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-31
• [Presentation] H+/有機カチオンアンチポークMATE1の機能低下による副作用発現の増強 (2012)
  • Author(s): 米澤淳, 他
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学、札幌市(招待講演)
  • Year and Date: 2012-03-31
• [Presentation] INVOLVEMENT OF HUMAN RIBOFLAVIN TRANSPORTERS(hRFTs) IN THE REGULATION OF INTRACELLULAR CONCENTRATION OF RIBOFLAVIN AND ITS ACTIVE FORMS (2011)
  • Author(s): Yoshiaki Yao, Atsushi Yonezawa, Hiroki Yoshimatsu, and Toshiya Katsura
  • Organizer: 26th JSSX Annual Meeting
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2011-11-16
• [Presentation] INVOLVEMENT OF HUMAN RIBOFLAVIN TRANSPORTERS (hRFTs) IN THE REGULATION OF INTRACELLULAR CONCENTRATION OF RIBOFLAVIN AND ITS ACTIVE FORMS (2011)
  • Author(s): Yao Y, et al
  • Organizer: 日本薬物動態学会第26回年会
  • Place of Presentation: 広島国際会議場、広島市
  • Year and Date: 2011-11-16
• [Presentation] Identification and fun ctional characterization of novel riboflavin transporters RFTs (2011)
  • Author(s): Atsushi Yonezawa, Yoshiaki Yao, Hiroki Yoshimatsu, Satohiro Masuda, Toshiya Katsura and Ken-ichi Inui
  • Organizer: Biomedical Transporters 2011
  • Place of Presentation: Grindelwald, Switzerland
  • Year and Date: 2011-08-10
• [Presentation] Identification and functional characterization of novel riboflavin transporters RFTs (2011)
  • Author(s): Yonezawa A, et al
  • Organizer: BioMedical Transporters 2011
  • Place of Presentation: グリンデルワルト、スイス
  • Year and Date: 2011-08-10
• [Presentation] ヒトリボフラビントランスポータhRFTの機能特性および病態生理学的役割の解明 (2011)
  • Author(s): 米澤淳、八尾祉顕、吉松宏樹、John Christodoulou、乾賢一、桂敏也
  • Organizer: 第6回トランスポータ研究会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-06-11
• [Presentation] ヒトリボフラビントランスポータhRFTの機能特性および病態生理学的役割の解明 (2011)
  • Author(s): 米澤淳, 他
  • Organizer: 第6回トランスポーター研究会年会
  • Place of Presentation: 東北大学、仙台市
  • Year and Date: 2011-06-11
• [Presentation] 腸由来T84細胞におけるヒトリボフラビントランスポータhRFTの役割 (2011)
  • Author(s): 吉松宏樹、米澤淳、八尾祉顕、乾賢一、桂敏也
  • Organizer: 日本薬剤学会第26年会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-29
• [Presentation] 腸由来T84細胞におけるヒトリボフラビントランスポータhRFTの役割 (2011)
  • Author(s): 吉松宏樹, 他
  • Organizer: 日本薬剤学会第26年会
  • Place of Presentation: タワーホール船堀、東京都
  • Year and Date: 2011-05-29
• [Presentation] Renal Organic Cation Transporter, OCT & MATE : From Drug Discovery to Clinical Applications. International symposium (2010)
  • Author(s): Atsushi Yonezawa, and Ken-ichi Inui
  • Organizer: An Interated Approach to the Physiology of Organic Cation Transporters
  • Place of Presentation: Gottingen, Deutschland
  • Year and Date: 2010-10-15
• [Presentation] Renal Organic Cation Transporter, OCT & MATE : From Drug Discovery to Clinical Applications. (2010)
  • Author(s): Yonezawa, A., et al.
  • Organizer: International symposium "An Interated Approach to the Physiology of Organic Cation Transporters
  • Place of Presentation: ゲッティンゲン、ドイツ(招待講演)
  • Year and Date: 2010-10-15
• [Presentation] INVOLVEMENT OF NOVEL HUMAN RIBOFLAVIN TRANSPORTERS hRFTs IN RIB OFLAVIN UPTAKE BY INTESTINALT84 CELLS (2010)
  • Author(s): Hiroki Yoshimatsu, Atsushi Yonezawa, Yoshiaki Yao, Satohiro Masuda, Toshiya Katsura and Ken-ichi Inu
  • Organizer: 25th JSSX Annual Meeting
  • Place of Presentation: Omiya, Japan
  • Year and Date: 2010-10-07
• [Presentation] INVOLVEMENT OF NOVEL HUMAN RIBOFLAVIN TRANSPORTERS hRFTs IN RIBOFLAVIN UPTAKE BY INTESTINAL T84 CELLS. (2010)
  • Author(s): Yoshimatsu, H., et al.
  • Organizer: 日本薬物動態学会第25年会
  • Place of Presentation: 大宮ソニックシティ、大宮市
  • Year and Date: 2010-10-07
• [Presentation] MOLECULAR CLONING AND COMPARATIVE FUNCTIONALCHARACTERIZATION OF HUMAN RIBOFLAVIN TRANSPORTERS RFTs. (2010)
  • Author(s): Atsushi Yonezawa, Yoshiaki Yao, Hiroki Yoshimatsu, Satohiro Masuda, Toshiya Katsura and Ken-ichi Inui
  • Organizer: 9th International Meeting of International Society for the Study of Xenobiotics
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2010-09-05
• [Presentation] MOLECULAR CLONING AND COMPARATIVE FUNCTIONAL CHARACTERIZATION OF HUMAN RIBOFLAVIN TRANSPORTERS RFTs (2010)
  • Author(s): Yonezawa, A., et al.
  • Organizer: 9th International Meeting of International Society for the Study of Xenobiotics
  • Place of Presentation: イスタンブール、トルコ
  • Year and Date: 2010-09-05
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/.yakuzai/practice.html