Development of measurement system of drug efflux transporter activity

• Project/Area Number: 22790156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Osaka University
• Principal Investigator: TSUGANE Mamiko 大阪大学, 大学院・薬学研究科, 助教 (00469991)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: マイクロチャンバー / トランスポーター / 抗がん剤 / 臨床薬学

Research Abstract

In this research, a system to measure the transportation efficiency of anti-cancer agents via ATP-binding cassette(ABC) transporter, which is involved in drug resistance, was developed. This system utilizes fluorescent imaging from a microchamber array fabricated using semiconductor microfabrication technology. Although a preliminary study of the system conditions was conducted using cultured cells, this system may possibly become the basic assay system for determining the drug transportation efficiency in cancer cells harvested from patients.