Development of screening method for albumin biomarker for personalized medicine

• Project/Area Number: 22790160
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: OHYAMA Kaname 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50437860)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 臨床化学 / HSA / 蛍光プローブ / 電気クロマトグラフィー / プロテオーム解析 / アルブミン

Research Abstract

Albumin with minor modification(i. e. Mercapto-albumin) are thought to be a target in fishing biomarker for several diseases and personalized medicine. A novel fluorescence probe specifically binding to human serum albumin and a polymer-monolithic capillary column for separation of the modified albumin were developed.

Research Products

• [Journal Article] Monolithic poly(butyl methacrylate-ethylene dimethacrylate-methacrylic acid) column for capillary electrochromatography (2011)
  • Author(s): K. Ohyama, D. Horiguchi, N. Kishikawa, N. Kuroda
  • Journal Title: Journal of Separation Science Volume: 34 Pages: 2279-2283
• [Journal Article] Immune complexome analysis of serum and its application in screening for immune complex antigens in rheumatoid arthritis (2011)
  • Author(s): K. Ohyama, Y. Ueki, A. Kawakami, N. Kishikawa, M. Tamai, M. Osaki, S. Kamihira, K. Nakashima, N. Kuroda*
  • Journal Title: Clinical Chemistry Volume: 57 Pages: 905-909
• [Journal Article] Monolithic poly(butyl methacrylate-ethylene dimethacrylate-methacrylicacid) column for capillary electrochromatography (2011)
  • Author(s): Kaname Ohyama, Daisuke Horiguchi, 他
  • Journal Title: Journal of Separation Science Volume: 34 Issue: 16-17 Pages: 2279-2283
  • DOI: 10.1002/jssc.201100198
  • Peer Reviewed
• [Journal Article] Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector (2010)
  • Author(s): N. Kishikawa, K. Ohyama, J. Yao, A. Miyamoto, T. Imazato, Y. Ueki, K. Nakashima, E. Maehata, N. Kuroda*
  • Journal Title: Clinica Chimica Acta Volume: 411 Pages: 1111-1115
  • NAID: 120006984533
• [Journal Article] A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity (2010)
  • Author(s): K. Ohyama, M. Tomonari, T. Ichibangase, H. To, N. Kishikawa, K. Nakashima, K. Imai, N. Kuroda
  • Journal Title: Biochemical Pharmacology Volume: 80 Pages: 540-547
  • NAID: 120006984488
• [Journal Article] Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector. (2010)
  • Author(s): Naoya Kishikawa, Kaname Ohyama, et al
  • Journal Title: Clinica Chimica Acta Volume: 411 Pages: 1111-1115
  • NAID: 120006984533
  • Peer Reviewed
• [Journal Article] A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity. (2010)
  • Author(s): Kaname Ohyama, et al
  • Journal Title: Biochemical Pharmacology Volume: 80 Pages: 540-547
  • NAID: 120006984488
  • Peer Reviewed
• [Presentation] 早期関節リウマチ患者のイムノコンプレキソーム解析 (2012)
  • Author(s): 大山要、馬場雅子、川上純, 他
  • Organizer: 第23回日本臨床化学会九州支部総会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-02-11
• [Presentation] 早期関節リウマチ患者のイムノコンプレキソーム解析 (2012)
  • Author(s): 大山要, 馬場雅子, 川上純, 玉井慎美, 岸川直哉, 植木幸孝, 上平憲, 中島憲一郎, 黒田直敬
  • Organizer: 第23回日本臨床化学会九州支部総会
  • Place of Presentation: 福岡
• [Presentation] 慢性関節リウマチ患者血清のLC-MS/MSによるイムノコンプレキソーム解析 (2011)
  • Author(s): 大山要、植木幸孝、川上純, 他
  • Organizer: 第18回クロマトグラフィーシンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 2011-06-02
• [Presentation] 慢性関節リウマチ患者血清のLC-MS/ MSによるイムノコンプレキソーム解析 (2011)
  • Author(s): 大山要, 植木幸孝, 川上純, 岸川直哉, 上平憲, 中島憲一郎, 黒田直敬
  • Organizer: 第18回クロマトグラフィーシンポジウム
  • Place of Presentation: 福岡
• [Presentation] Characterization of peptide chiral selectors prepared by solid-phase synthesis in HPLC enantioseparation. (2010)
  • Author(s): Kaname Ohyama, et al
  • Organizer: 22^<th> International Symposium on Chirality
  • Place of Presentation: 札幌
  • Year and Date: 2010-07-14
• [Presentation] メタクリル酸をイオン性モノマーとするCEC用モノリスカラムの評価 (2010)
  • Author(s): 大山要, 堀口大輔, 岸川直哉, 中島憲一郎, 黒田直敬
  • Organizer: 第21回クロマトグラフィー科学会議
  • Place of Presentation: 西宮
• [Presentation] Characterization of peptide chiral selectors prepared by solid-phase synthesis in HPLC enantioseparation (2010)
  • Author(s): K. Ohyama, K. Oyamada, N. Kishikawa, Y. Ohba, M. Wada, K. Nakashima, N. Kuroda
  • Organizer: 22^〈th〉International Symposium on Chirality
  • Place of Presentation: Sapporo, Japan
• [Remarks]
  • URL: http://www.ph.nagasaki-u.ac.jp/lab/analysis/index-j.html
• [Patent(Industrial Property Rights)] 免疫複合体の網羅的解析方法および新規関節リウマチバイオマーカー (2011)
  • Inventor(s): 黒田直敬,大山要,岸川直哉
  • Industrial Property Rights Holder: 国立大学法人長崎大学
  • Industrial Property Number: 2011-215402
  • Filing Date: 2011-09-29
• [Patent(Industrial Property Rights)] 免疫複合体の網羅的解析方法および新規関節リウマチバイオマーカー (2011)
  • Inventor(s): 黒田直敬, 大山要, 岸川直哉
  • Industrial Property Rights Holder: 国立大学法人長崎大学
  • Industrial Property Number: 2011-215402
  • Filing Date: 2011-09-29
• [Patent(Industrial Property Rights)] 免疫複合体の網羅的解析方法および新規関節リウマチバイオマーカー (2010)
  • Inventor(s): 黒田直敬, 大山要, 岸川直哉
  • Industrial Property Rights Holder: 国立大学法人長崎大学
  • Industrial Property Number: 2010-231935
  • Filing Date: 2010-10-14