| Budget Amount *help |
¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Primary human hepatocytes are used extensively to study drug-metabolizing enzymes such as the cytochrome P450 enzymes. However, the activities of these enzymes decrease rapidly during culture. Thus, a more functional culture system is required to obtain the usually high levels of activity and regulation ability of drug metabolizing systems. In the present study, using a thermo-responsive culture dish, layered co-culture was achieved by placing a pulmonary artery endothelial cell sheet onto the human small hepatocyte. The expression levels of drug-metabolizing enzymes were significantly increased, compared with the monolayer cultured small hepatocytes.
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