Neurogenesis in the adult spinal cord by cell fate control

• Project/Area Number: 22790181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tohoku University
• Principal Investigator: KITADA Masaaki 東北大学, 大学院・医学系研究科, 准教授 (80324614)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脊髄損傷 / グリア前駆細胞 / 上衣細胞 / オリゴデンドロサイト前駆細胞 / 神経細胞新生

Research Abstract

We have studied the property of the glial progenitor cells in the adult spinal cord. The ependymal cells in the intact spinal cord expressed the factor A, which was up-regulated after spinal cord injury. We created an adenovirus vector encoding microRNA targeted to the factor A and injected into the ventricle. We found the ependymal cell-derived neurons. The ependymal cells in the adult spinal cord are demonstrated to produce neurons by this cell fate control method.

Research Products

• [Journal Article] Transplantation of bone marrow stromal cells-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection (2013)
  • Author(s): Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera J J, Kawashima R, Tominaga T, Dezawa M.
  • Journal Title: Cell Transplant Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques (2013)
  • Author(s): Hayashi T, Wakao S, Kitada M, Ose T, Watabe H, Kuroda Y, Mitsunaga K, Matsuse D, Shigemoto T, Ito A, Ikeda H, Fukuyama H, Onoe H, Tabata Y, Dezawa M
  • Journal Title: J Clin Invest Volume: 123(1) Issue: 1 Pages: 272-284
  • DOI: 10.1172/jci62516
  • Peer Reviewed
• [Journal Article] The elite and stochastic model for iPS cell generation: multilineage-differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells (2013)
  • Author(s): Wakao S, Kitada M, Dezawa M.
  • Journal Title: Cytometry A Volume: 83 Issue: 1 Pages: 18-26
  • DOI: 10.1002/cyto.a.22069
  • Peer Reviewed
• [Journal Article] Morphologic and gene expression criteria for identifying human induced pluripotent stem cells (2012)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Ogura F, Murakami T, Niwa A, Dezawa M.
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: e48677-e48677
  • DOI: 10.1371/journal.pone.0048677
  • Peer Reviewed
• [Journal Article] Muse cells and induced pluripotent stem cell: implication of the elite model (2012)
  • Author(s): Kitada M, Wakao S, Dezawa M.
  • Journal Title: Cell MolLife Sci Volume: 69 Issue: 22 Pages: 3739-3750
  • DOI: 10.1007/s00018-012-0994-5
  • Peer Reviewed
• [Journal Article] Parkinson's Disease and Mesenchymal Stem Cells: Potential for Cell-Based Therapy (2012)
  • Author(s): Kitada M, Dezawa M.
  • Journal Title: Parkinson's Disease Volume: - Pages: 873706-873706
  • DOI: 10.1155/2012/873706
  • Peer Reviewed
• [Journal Article] Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages (2012)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Dezawa M
  • Journal Title: Liver stem Cells : Methods and Protocols, Methods in Molecular Biology Volume: 826(2) Pages: 89-102
  • DOI: 10.1007/978-1-61779-468-1_8
  • ISBN: 9781617794674, 9781617794681
  • Peer Reviewed
• [Journal Article] Transplantation of bone marrow stromal cells-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection (2012)
  • Author(s): Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera J J, Kawashima R, Tominaga T, Dezawa M
  • Journal Title: Cell Transplant Volume: 掲載確定
  • Peer Reviewed
• [Journal Article] 視神経の再生 (2012)
  • Author(s): 北田容章、出澤真理
  • Journal Title: 長寿科学研究業績週2011 Volume: 2011(in press)
• [Journal Article] Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and basic fibroblast growth factor releasing microspheres enhances recovery after cerebral ischemia in rats (2011)
  • Author(s): Matsuse D, Kitada M, Ogura F, Wakao S, Kohama M, Kira J, Tabata Y, Dezawa M.
  • Journal Title: Tissue Eng Part Volume: A 17 Issue: 15-16 Pages: 1993-2004
  • DOI: 10.1089/ten.tea.2010.0585
  • Peer Reviewed
• [Journal Article] Multiliineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts (2011)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Shigemoto T, Matsuse D, Akashi H, Tanimura Y, Tsuchiyama K, Kikuchi T, Goda M, Nakahata T, Fijiyoshi Y, Dezawa M
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. Volume: 108(24) Issue: 24 Pages: 9875-9880
  • DOI: 10.1073/pnas.1100816108
  • Peer Reviewed
• [Journal Article] Lectins as a tool for detecting neural stem/progenitor cells in the adult mouse brain (2011)
  • Author(s): Kitada M, Kuroda Y, Dezawa M
  • Journal Title: Anat.Rec.(Hoboken) Volume: 294(2) Issue: 2 Pages: 305-321
  • DOI: 10.1002/ar.21311
  • Peer Reviewed
• [Journal Article] Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration (2011)
  • Author(s): Wakao S, Hayashi T, Kitada M, Kohama M, Matsuse D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, Iida H, Takamoto T, Tabata Y, Dezawa M.
  • Journal Title: Exp Neurol Volume: 223 Issue: 2 Pages: 537-547
  • DOI: 10.1016/j.expneurol.2010.01.022
  • Peer Reviewed
• [Journal Article] Bone Marrow Mesenchymal Cells : How Do They Contribute to Tissue Repair and Are They Really Stem Cells? (2011)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Dezawa M
  • Journal Title: Arch.Immunol.Ther.Exp.(Warsz) Volume: 59(5) Issue: 5 Pages: 369-378
  • DOI: 10.1007/s00005-011-0139-9
  • Peer Reviewed
• [Journal Article] Mesenchymal cell populations: development of the induction systems for Schwann cells and neuronal cells and finding the unique stem cell population (2011)
  • Author(s): Kitada M.
  • Journal Title: Anat Sci Int Volume: 87 Issue: 1 Pages: 24-44
  • DOI: 10.1007/s12565-011-0128-4
  • NAID: 10031162255
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells and umbilical cord as sources for Schwann cell differentiation: their potential in peripheral nerve repair (2011)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Dezawa, M.
  • Journal Title: The Open Tissue Engineering and Regenerative Medicine Journal Volume: 4 Issue: 1 Pages: 54-63
  • DOI: 10.2174/1875043501104010054
  • Peer Reviewed
• [Journal Article] Lectins as a tool fro detecting neural stem/progenitor cells in the adult mouse brain (2011)
  • Author(s): Kitada M, Kuroda Y, Dezawa M
  • Journal Title: Anat Rec Volume: 294(2) Pages: 305-321
  • Peer Reviewed
• [Journal Article] Unique multipotent cells in adult human mesenchymal cell populations (2010)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, Makinoshima H, Goda M, Akashi H, Inutsuka A, Niwa A, Shigemoto T, Nabeshima Y, Nakahata T, Nebeshima Y, Fujiyoshi Y, Dezawa M.
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Issue: 19 Pages: 8639-8643
  • DOI: 10.1073/pnas.0911647107
  • Peer Reviewed
• [Journal Article] Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration (2010)
  • Author(s): Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S, Fujiyoshi Y, Heike T, Nakahata T, Akutsu H, Umezawa A, Harigae H, Kira J, Dezawa M.
  • Journal Title: J Neuropathol Exp Neurol Volume: 69 Issue: 9 Pages: 973-985
  • DOI: 10.1097/nen.0b013e3181eff6dc
  • NAID: 10030408600
  • Peer Reviewed
• [Journal Article] Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration (2010)
  • Author(s): Wakao S, Hayashi T, Kitada M, Kohama M, Matsuse D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, Iida H, Takamoto T, Tabata Y, Dezawa M
  • Journal Title: Exp Neurol Volume: 223(2) Pages: 537-547
  • Peer Reviewed
• [Journal Article] Unique multipotent cells in adult human mesenchymal cell populations (2010)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, Makinoshima H, Goda M, Akashi H, Inutsuka A, Niwa A, Shigemoto T, Nabeshima Y, Nakahata T, Nebeshima Y, Fujiyoshi Y, Dezawa M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(19) Pages: 8639-8643
  • Peer Reviewed
• [Journal Article] Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration (2010)
  • Author(s): Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S, Fujiyoshi Y, Heike T, Nakahata T, Akutsu H, Umezawa A, Harigae H, Kira J, Dezawa M
  • Journal Title: J Neuropathol Exp Neurol Volume: 69(9) Pages: 973-985
  • NAID: 10030408600
  • Peer Reviewed
• [Presentation] 成体ラット脊髄上衣細胞の生体内細胞運命調節の試み (2013)
  • Author(s): 北田容章
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-29
• [Presentation] 内在性幹・前駆細胞の賦活化による新規脊髄損傷治療法の開発 (2013)
  • Author(s): 北田容章
  • Organizer: 第1回In vivo再生研究会
  • Place of Presentation: 東京都
  • Year and Date: 2013-03-24
• [Presentation] Cell fate intervention of the progenitor cells in the adult rodent spinal cord (2013)
  • Author(s): Kitada M
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: 名古屋市
• [Presentation] 成体ラット脊髄上衣細胞の生体内細胞運命調節の試み (2013)
  • Author(s): 北田容章
  • Organizer: 第118回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松市
• [Presentation] 哺乳類における脊髄再生への挑戦-「発生学的に正しい」再生への道- (2012)
  • Author(s): 北田容章
  • Organizer: 第83回大会2012大阪(招待講演)
  • Place of Presentation: 大阪市
  • Year and Date: 2012-09-14
• [Presentation] 成体ラット脊髄上衣細胞の分裂・移動様式の解明 (2012)
  • Author(s): 北田容章
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-13
• [Presentation] 遺伝学的手法を用いない生体内細胞系譜追跡実験法 (2012)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば市
  • Year and Date: 2012-05-14
• [Presentation] 成体ラット脊髄上衣細胞の正常時・損傷時における動態 (2012)
  • Author(s): 北田容章
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] 新たな多能性幹細胞であるMuse細胞の可能性 (2012)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会バイオメディカルニューマイクロスコープ分科会平成23年度シンポジウム講演会
  • Place of Presentation: 板橋区
  • Year and Date: 2012-03-06
• [Presentation] 遺伝学的手法を用いない生体内細胞系譜追跡実験法 (2012)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会 第68回学術講演会
  • Place of Presentation: つくば市
• [Presentation] 成体ラット脊髄上衣細胞の分裂・移動様式の解明 (2012)
  • Author(s): 北田容章
  • Organizer: 第11回 日本再生医療学会総会
  • Place of Presentation: 横浜市
• [Presentation] 哺乳類における脊髄再生への挑戦 -「発生学的に正しい」再生への道 - (2012)
  • Author(s): 北田容章
  • Organizer: 日本動物学会 第83回大会 2012大阪
  • Place of Presentation: 大阪市
  • Invited
• [Presentation] Stem/progenitor cell property of the ependymal cells in the adult rodent spinal cord (2011)
  • Author(s): Kitada M
  • Organizer: The 21st CDB Regeneration biology Study Group Meeting #1
  • Place of Presentation: Kobe
  • Year and Date: 2011-11-24
• [Presentation] 成体ラット脊髄DM-20発現細胞のオリゴデンドロサイト系譜前駆細胞としての可能性 (2011)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-05-16
• [Presentation] Muse細胞ー新たな多能性幹細胞の発見ー (2010)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会第54回シンポジウム
  • Place of Presentation: 金沢市
  • Year and Date: 2010-11-12
• [Presentation] Muse細胞-新たな多能性幹細胞の発見- (2010)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会第54回シンポジウム
  • Place of Presentation: 金沢市
  • Year and Date: 2010-11-12
• [Presentation] 成体脊髄に存在する新たなオリゴデンドロサイト系譜前駆細胞の同定 (2010)
  • Author(s): 北田容章
  • Organizer: 日本解剖学会第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川市
  • Year and Date: 2010-09-25
• [Presentation] 成体脊髄におけるグリア前駆細胞の動態解析 (2010)
  • Author(s): 北田容章
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-05-24
• [Presentation] 内在性幹・前駆細胞の賦活化による新規脊髄損傷治療法の開発
  • Author(s): 北田容章
  • Organizer: 第１回 In vivo再生研究会
  • Place of Presentation: 東京都
• [Book] 第11章間葉系幹細胞・Muse細胞を用いた再生医療/再生医療叢書第7巻神経系 (2013)
  • Author(s): 北田容章・出澤真理
  • Publisher: 朝倉書店
• [Book] 第１１章 間葉系幹細胞・Muse細胞を用いた再生医療／再生医療叢書 第７巻 神経系 (2013)
  • Author(s): 北田容章
  • Publisher: 朝倉書店
• [Book] 視覚障害者の再生医療視神経の再生/高齢者の視覚障害とそのケアAdvances in Aging and Health Research 2011 (2012)
  • Author(s): 北田容章・出澤真理
  • Publisher: 公益財団法人長寿科学振興財団
• [Book] 視覚障害者の再生医療 視神経の再生／高齢者の視覚障害とそのケア Advances in Aging and Health Research 2011 (2012)
  • Author(s): 北田容章
  • Publisher: 公益財団法人 長寿科学振興財団