Transcytosis of cathepsin D in nervous system.

• Project/Area Number: 22790183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: SHIBATA Masahiro 新潟大学, 医歯学系, 准教授 (10343253)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リソソーム / カテプシン / ライソゾーム病 / セロイドリポフスチノーシス / カテプシンD / トランスポゾン

Research Abstract

A chick model to monitor the transcytosis of lysosomal enzymes in vivo was developed. I have previously shown that the stable integration of transgenes into chick genome using Tol2 transposition allows to trace astrocytes from the early embryo to late developmental stage near hatching. In this study, a conditional gene knockdown technique driven by U6 promoter using a combination of Cre-loxP system and Tol2 transposition was developed.

Research Products

• [Journal Article] Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles (2011)
  • Author(s): Yamazaki Y, Shibata M, Ushiki T, Isokawa K, Sato N
  • Journal Title: J Oral Sci Volume: 53(4) Pages: 523-7
  • NAID: 130001381392
  • Peer Reviewed
• [Journal Article] Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgical protocols (2011)
  • Author(s): Matsuo T, Kawasaki K, Osada T, Sawahata H, Suzuki T, Shibata M, Miyakawa N, Nakahara K, Iijima A, Sato N, Kawai K, Saito N, Hasegawa I
  • Journal Title: Front Syst Neurosci Volume: 5 Pages: 34-34
  • Peer Reviewed
• [Journal Article] Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice (2011)
  • Author(s): Shiozaki M, Hayakawa N, Shibata M, Koike M, Uchiyama Y, Gotow T
  • Journal Title: Histochem Cell Biol1 Volume: 136(4) Pages: 475-89
  • Peer Reviewed
• [Journal Article] Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice (2011)
  • Author(s): Shiozaki M., Hayakawa N., Shibata M., Koike M., Uchiyama Y., Gotow T.
  • Journal Title: Histochem. Cell Biol Volume: 136 Issue: 4 Pages: 475-489
  • DOI: 10.1007/s00418-011-0847-6
  • Peer Reviewed
• [Journal Article] Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgicalprotocols (2011)
  • Author(s): Matsuo T, Kawasaki K, Osada T, SawahataH, Suzuki T, Shibata M, Miyakawa N, Nakahara K, Iijima A, Sato N, Kawai K, Saito N, Hasegawa I
  • Journal Title: Front Syst Neurosci Volume: 5 Pages: 34-34
  • DOI: 10.3389/fnsys.2011.00034
  • Peer Reviewed
• [Journal Article] Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles (2011)
  • Author(s): Yamazaki, Y.
  • Journal Title: Journal of Oral Science Volume: 53 Issue: 4 Pages: 523-527
  • DOI: 10.2334/josnusd.53.523
  • NAID: 130001381392
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed
• [Journal Article] LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation (2010)
  • Author(s): Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y
  • Journal Title: Biochem Biophys Res Commun Volume: 393(2) Pages: 274-9
  • Peer Reviewed
• [Journal Article] Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains (2010)
  • Author(s): Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y
  • Journal Title: J Histochem Cytochem Volume: 58(5) Pages: 443-53
  • Peer Reviewed
• [Journal Article] LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation. (2010)
  • Author(s): Shibata M, et al
  • Journal Title: Biochem Biophys Res Commun. Volume: 393 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains. (2010)
  • Author(s): Tamura H, et al
  • Journal Title: J Histochem Cytochem Volume: 58 Pages: 443-453
  • Peer Reviewed
• [Journal Article] The MAP1-LC3 conjugation system is involved in lipid droplet formation (2009)
  • Author(s): Shibata M, Yoshimura K, Furuya N, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y
  • Journal Title: Biochem Biophys Res Commun Volume: 382(2) Pages: 419-23
  • Peer Reviewed
• [Presentation] 細胞の脱分化過程を観察する試み (2012)
  • Author(s): 平林茂樹、伊藤健二朗、柴田昌宏、佐藤昇
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学(甲府、山梨県)
  • Year and Date: 2012-03-26
• [Presentation] Gene transfer into the chick by using Tol2 transposition for studying the nervous system development. (2011)
  • Author(s): 柴田昌宏、佐藤昇
  • Organizer: 第8回国際脳研究機構会議
  • Place of Presentation: フィレンツェ、イタリア
  • Year and Date: 2011-07-16
• [Presentation] Gene transfer into the chick by using Tol2 transposition for studying the nervous system development (2011)
  • Author(s): Shibata, M., Sato, N.
  • Organizer: IBRO
  • Place of Presentation: フィレンツェ(イタリア)
  • Year and Date: 2011-07-16
• [Presentation] In vivo imaging of the chick using tol2 transposition (2011)
  • Author(s): 柴田昌宏、伊藤健二朗、佐藤昇
  • Organizer: 第116回日本解剖学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-03-30
• [Presentation] In vivo imaging of the chick using tol2 transposition. (2011)
  • Author(s): 柴田昌宏、伊藤健二朗、佐藤昇
  • Organizer: 日本解剖学会
  • Place of Presentation: パシフィコ横浜(震災のため紙面開催)
• [Presentation] Conditional RNA interference using a combination of Cre-loxP system and Tol2 transposition is a useful tool for the developmental studies in the chick (2010)
  • Author(s): 柴田昌宏、伊藤健二朗、佐藤昇
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸コンベンションセンター