| Project/Area Number |
22790195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
MIYOSHI Kan 兵庫医科大学, 医学部, 助教 (30454755)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Caspase-1 / IL-18 / microglia / neuropathic pain / spinal cord / astrocytes / glial interaction / caspase-1 / astrocyte / TLR4 / IL-1β |
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| Research Abstract |
SNL induced an increase in the expression of IL-18 and caspase-1 in microglia, in the spinal dorsal horn. Intrathecal injection of the TLR4 agonist, LPS, produced upregulation of Iba1, caspase-1, and IL-18 expression, which was prevented by a p38 MAPK inhibitor. Both anti-IL-18 antibody and caspase-1 inhibitors alleviated SNL-induced tactile allodynia. Conversely, intrathecal injection of IL-18 produced both tactile allodynia. IL-18-deficient mice or caspase-1-deficient mice did not develop tactile allodynia after SNL. Thus, blocking IL-18 signaling in glial cells might provide a fruitful strategy for treating neuropathic pain.
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