Analysis of unconventional orientation of MaxiK proteins in pancreatic acinar ER membrane

• Project/Area Number: 22790198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: MURATA Yoshimichi 東北大学, 大学院・医学系研究科, 助教 (60455780)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生体膜 / イオンチャネル / 小胞体膜

Research Abstract

We prepared nuclear envelopes (NEs)from mouse pancreatic acinar cells and observed MaxiK currents in the membrane subjected to the patch-clamp technique. The results indicate that the MaxiK channels are oriented in the ER membrane as their usual extracellular side faces to cytosol. These observations are inconsistent with the conventional understandings of the protein transport process.

Research Products

• [Journal Article] Voltage-dependent biphasic effects of chloroquine on delayed rectifier K^+-channel currents in murine thymocytes (2012)
  • Author(s): 風間逸郎、丸山芳夫、村田喜理
  • Journal Title: Journal of Physiological Science Volume: (印刷中) Issue: 3 Pages: 267-274
  • DOI: 10.1007/s12576-012-0195-x
  • Peer Reviewed
• [Presentation] Orientation of K^+ channel proteins in the peri-nuclear ER membrane of HEK293 cells Part2 (2012)
  • Author(s): 村田喜理、風間逸郎、丸山芳夫
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本市
  • Year and Date: 2012-03-29
• [Presentation] Orientation of K+ channel proteins in the peri-nuclear ER membrane of HEK293 cells Part2 (2012)
  • Author(s): 村田喜理
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本市総合体育館
  • Year and Date: 2012-03-29
• [Presentation] Orientation of K^+ channel proteins in the peri-nuclear ER membrane of HEK293 cells (2011)
  • Author(s): 村田喜理、風間逸郎、丸山芳夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-09-17
• [Presentation] Orientation of K+ channel proteins in the peri-nuclear ER membrane of HEK293 cells (2011)
  • Author(s): 村田喜理
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Effects of membrane amphipath on delayed rectifier K^+-channel currents in megakaryocytes (2011)
  • Author(s): 風間逸郎、村田喜理、丸山芳夫
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: Journal of Physiological science誌上(東日本大震災による)
  • Year and Date: 2011-03-29
• [Presentation] Effects of membrane amphipath on delayed rectifier K+-channel currents in megakaryocytes (2011)
  • Author(s): 風間逸郎
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: Journal of Physiological science誌上(東日本大震災による)
  • Year and Date: 2011-03-29
• [Presentation] Orientation of MaxiK channels in the peri-nuclear ER membrane : comparison of HEK293 cells and pancreatic acinar cells (2011)
  • Author(s): 村田喜理、風間逸郎、丸山芳夫
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: Journal of Physiological science誌上(東日本大震災による)
  • Year and Date: 2011-03-28
• [Presentation] Orientation of MaxiK channels in the peri-nuclear ER membrane : comparison of HEK293 cells and pancreatic acinar cells (2011)
  • Author(s): 村田喜理
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: Journal of Physiological science誌上(東日本大震災による)
  • Year and Date: 2011-03-28